Aflatoxin M1 Determination in Whole Milk with Immersible Silicon Photonic Immunosensor DOI Creative Commons

Dimitra Kourti,

Μichailia Angelopoulou,

Eleni Makarona

et al.

Toxins, Journal Year: 2025, Volume and Issue: 17(4), P. 165 - 165

Published: March 26, 2025

Aflatoxin M1 (AFM1) appears in the milk of animals that have consumed feed contaminated with aflatoxin B1. AFM1 presence is regulated by European Commission, which has set maximum allowable limits for adult and infant consumption to 50 25 pg/mL, respectively. Here, a rapid sensitive method detecting based on an immersible silicon photonic chip presented. The features two U-shaped nitride waveguides formed as Mach–Zehnder interferometers. One interferometer functionalized AFM1–bovine serum albumin conjugate other BSA serve blank. connected broad-band white LED spectrophotometer bifurcated optical fiber assay performed immersing mixture anti-AFM1 antibody. Then, sequentially immersed biotinylated anti-rabbit IgG antibody streptavidin solutions signal enhancement. completed 20 min detection limit undiluted pg/mL. Given its analytical performance absence pumps fluidics lead compact instrument design, proposed immunosensor ideal on-site samples.

Language: Английский

Aflatoxin M1 Determination in Whole Milk with Immersible Silicon Photonic Immunosensor DOI Creative Commons

Dimitra Kourti,

Μichailia Angelopoulou,

Eleni Makarona

et al.

Toxins, Journal Year: 2025, Volume and Issue: 17(4), P. 165 - 165

Published: March 26, 2025

Aflatoxin M1 (AFM1) appears in the milk of animals that have consumed feed contaminated with aflatoxin B1. AFM1 presence is regulated by European Commission, which has set maximum allowable limits for adult and infant consumption to 50 25 pg/mL, respectively. Here, a rapid sensitive method detecting based on an immersible silicon photonic chip presented. The features two U-shaped nitride waveguides formed as Mach–Zehnder interferometers. One interferometer functionalized AFM1–bovine serum albumin conjugate other BSA serve blank. connected broad-band white LED spectrophotometer bifurcated optical fiber assay performed immersing mixture anti-AFM1 antibody. Then, sequentially immersed biotinylated anti-rabbit IgG antibody streptavidin solutions signal enhancement. completed 20 min detection limit undiluted pg/mL. Given its analytical performance absence pumps fluidics lead compact instrument design, proposed immunosensor ideal on-site samples.

Language: Английский

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