A Metal–Organic Hybrid Composed of Dual Quenching Cofactors as a Nanoquencher for the Fluorescent Determination of Protease Caspase-3 DOI Creative Commons

Fengli Gao,

Lin Liu, Cheng-Ru He

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(6), P. 354 - 354

Published: June 4, 2025

Nanoquenchers with a single quenching cofactor exhibit limited fluorescence efficiency. In this work, metal–organic hybrid dual cofactors (Cu2+ and pyrroloquinoline quinone or PQQ) was prepared by metal-coordinated assembly used as nanoquencher for protease assay enhanced The peptide substrate an oligohistidine (His6) tag labeled fluorophore. Caspase-3 determined example. attached onto the surface of Cu-PQQ metal coordination interaction between unsaturated Cu2+ on nanoparticle His6 in peptide. cleavage enzymatic hydrolysis led to release fluorophore-conjugated segment from surface, thus turning fluorescence. nanoprobe determine caspase-3 linear range 0.01–5 ng/mL detection limit 7 pg/mL. Furthermore, method evaluate inhibition efficiency monitor drug-induced cell apoptosis. contrast other means immobilization, such physical adsorption covalent coupling, strategy based is simple powerful, thereby achieving assays activity lysates satisfactory result. work should be valuable design nanoquenchers multiple development novel biosensors.

Language: Английский

Portable pH meter-based competitive immunoassay of E-selectin using urease-encapsulated metal-organic frameworks DOI
Yiyan Xie,

Xianhuan He,

Rujie Yu

et al.

Talanta, Journal Year: 2025, Volume and Issue: 287, P. 127613 - 127613

Published: Jan. 18, 2025

Language: Английский

Citations

0
Novel GC MS/MS method for bioanalysis of pyrroloquinoline quinone, a potential cognitive enhancer in mice brains DOI

Mai Mostafa,

Reham M. Abdel‐Kader, Rasha S. Hanafi

et al.

Journal of Chromatography B, Journal Year: 2025, Volume and Issue: unknown, P. 124559 - 124559

Published: March 1, 2025

Language: Английский

Citations

0
A Review of Recent Trends in the Development of Analytical Methods for Useful Compounds and Contaminations in Fruits Juices DOI
Ebraheem Abdu Musad Saleh, Hiba Muwafaq Saleem, Mohammed Asiri

et al.

Critical Reviews in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 20

Published: March 26, 2025

In modern lifestyles, the consumption of fruit juice is increasing among different societies. Many people consider juices to be safe beverages with numerous health benefits for human bodies. Although contain several useful compounds that can promote health, there a probability both microbiological and non-microbiological contaminations in these juices. It important have reliable detection methods analyze harmful present industry, various analytical been used analyzing parameters, contaminations, However, some limitations restrict their application this field. Biosensors, as newly emerging sensing approaches, developed The sensitivity, selectivity, portability, accuracy are key considerations developing biosensors antioxidants. This was achieved by bioreceptors, nanomaterials, novel designed technologies. review, role techniques, bioreceptors reviewed development new terms addition, future prospects challenges also examined.

Language: Английский

Citations

0
Nonlocal optical field effects in metal nanoplasmonics influenced by dielectric environments DOI Creative Commons
Xiaoming Li,

Huan Ren,

Zhi Min Cui

et al.

AIP Advances, Journal Year: 2025, Volume and Issue: 15(4)

Published: April 1, 2025

Metal nanoplasmonics has emerged as a transformative platform for sub-diffraction-limited light manipulation, owing to its unique capability confine and enhance optical fields at the nanoscale. While significant progress been made in understanding plasmon-enhanced phenomena over past two decades, theoretical investigations into how nanoscale dielectric environments influence properties of metallic plasmonic nanostructures remain limited. In this work, we employ quantum hydrodynamic model develop unified approach analyzing nonlocal field effects metal–dielectric nanowire systems. Our findings demonstrate that permittivity exerts long-range on resonance frequency nanostructures, with higher constants inducing redshift plasmon frequency. Furthermore, inter-wire spacing between nanowires critically affects distribution enhancement. Notably, decreasing dielectric–metal proximity does not universally light-field amplification. A primary factor contributing observation is interfacial screening alters mode’s effective wavelength. These results promise establish foundational design principles advanced nanophotonic devices.

Language: Английский

Citations

0
Sulfide regulation and catabolism in health and disease DOI Creative Commons
Yuanyuan Hou,

Boyang Lv,

Junbao Du

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)

Published: May 30, 2025

Abstract The metabolic pathway of sulfur-containing amino acids in organisms begins with methionine, which is metabolized to produce important biomolecules such as adenosylmethionine, adenosylhomocysteine, homocysteine, cystine, and hydrogen sulfide (H 2 S). These play a wide range physiological roles the body, including anti-inflammation, antioxidant stress, DNA methylation, protein synthesis, etc., are essential for maintaining cellular function overall health. In contrast, dysregulation leads abnormal levels biomolecules, pathological consequences multiple systems neurodegenerative diseases, cardiovascular cancer. This review traces milestones development these from their initial discovery clinical applications describes detail structure, physiochemical properties, metabolism, signaling pathway, physiopathological functions, assays biomolecules. addition, paper also explores regulatory role mechanism on liver neurological diseases tumors. focus placed donors biological macromolecule metabolites, small-molecule drug screening targeting H S-producing enzymes, latest advancements preclinical research related sulfide, trials FDA-approved drugs. Additionally, an overview future directions this field provided. aim enhance understanding complex offer insights into developing effective therapeutic strategies associated dysregulated acid metabolism.

Language: Английский

Citations

0
A Metal–Organic Hybrid Composed of Dual Quenching Cofactors as a Nanoquencher for the Fluorescent Determination of Protease Caspase-3 DOI Creative Commons

Fengli Gao,

Lin Liu, Cheng-Ru He

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(6), P. 354 - 354

Published: June 4, 2025

Nanoquenchers with a single quenching cofactor exhibit limited fluorescence efficiency. In this work, metal–organic hybrid dual cofactors (Cu2+ and pyrroloquinoline quinone or PQQ) was prepared by metal-coordinated assembly used as nanoquencher for protease assay enhanced The peptide substrate an oligohistidine (His6) tag labeled fluorophore. Caspase-3 determined example. attached onto the surface of Cu-PQQ metal coordination interaction between unsaturated Cu2+ on nanoparticle His6 in peptide. cleavage enzymatic hydrolysis led to release fluorophore-conjugated segment from surface, thus turning fluorescence. nanoprobe determine caspase-3 linear range 0.01–5 ng/mL detection limit 7 pg/mL. Furthermore, method evaluate inhibition efficiency monitor drug-induced cell apoptosis. contrast other means immobilization, such physical adsorption covalent coupling, strategy based is simple powerful, thereby achieving assays activity lysates satisfactory result. work should be valuable design nanoquenchers multiple development novel biosensors.

Language: Английский

Citations

0