Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits

Foods, Journal Year: 2023, Volume and Issue: 12(7), P. 1505 - 1505

Published: April 3, 2023

A comprehensive characterization of the phytochemicals present in a blackberry fruit extract by HPLC-TOF-MS has been carried out. The main compounds were ursane-type terpenoids which, along with phenolic compounds, may be responsible for bioactivity extract. In vitro antioxidant capacity was assessed through Folin-Ciocalteu (31.05 ± 4.9 mg GAE/g d.w.), FRAP (637.8 3.2 μmol Fe2+/g DPPH (IC50 97.1 2.4 μg d.w./mL) and TEAC (576.6 8.3 TE/g d.w.) assays. Furthermore, exerted remarkable effects on cellular activity HUVEC cells at concentration 5 mg/mL. Antimicrobial also tested. Most sensible microorganisms Gram-positive bacteria, such as E. faecalis, B. cereus Gram-negative coli (MBC 12.5 mg/mL). IC50 values against colon tumoral HT-29 (4.9 0.2 mg/mL), T-84 (5.9 0.3 mg/mL) SW-837 obtained. demonstrated anti-inflammatory inhibiting secretion pro-inflammatory IL-8 cytokines two models (HT-29 T-84) concentration-dependent manner. These results support that fruits are an interesting source bioactive useful prevention treatment different diseases, mainly related to oxidative stress.

Language: Английский

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Quality Standards and Pharmacological Interventions of Natural Oils: Current Scenario and Future Perspectives

ACS Omega, Journal Year: 2023, Volume and Issue: 8(43), P. 39945 - 39963

Published: Oct. 17, 2023

Medicinal plants are rich sources of natural oils such as essential and fixed used traditionally for nutritive well medicinal purposes. Most the traditional formulations or phytopharmaceutical contain oil main ingredient due to their own therapeutic applications thus mitigating several pathogeneses fungal/bacterial/viral infection, gout, psoriasis, analgesic, antioxidant, skin etc. Due lack quality standards progressive adulteration in oils, efficacy is continuously deteriorated. To develop validate scientific aspects on chromatographic spectroscopic techniques HPTLC, HPLC, NMR, LC–MS, GC–MS have been termed choices better exploration metabolites, hence sustaining authenticity oils. In this review, chemical profiling control explored from previously reported literature reputed journals. Methods profiling, possible identified metabolites described. The outcome review reveals that GC–MS/MS, LC-MS/MS, NMR-based most liable, economic, precise, accurate determining spuriousness based qualitative quantitative studies. This occupies extensive information about obtained regulatory via providing detailed methods chemoprofiling techniques. Hence, helps researchers further quality-based standardization purpose.

Language: Английский

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COVID-19 pandemic sheds a new research spotlight on antiviral potential of essential oils – A bibliometric study

Heliyon, Journal Year: 2023, Volume and Issue: 9(7), P. e17703 - e17703

Published: June 30, 2023

Essential oils are thought as potential therapies in managing coronavirus disease 2019 (COVID-19). Many researchers have put their efforts to tackle the pandemic by exploring antiviral candidates which consequently changes research landscape. Herein, we aimed assess effect of COVID-19 toward landscape essential oil research.

Language: Английский

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Randomised, Placebo-Controlled, Double-Blind Trial to Assess Efficacy and Safety of ELOM-080 in Outpatients with COVID-19

Advances in Therapy, Journal Year: 2025, Volume and Issue: 42(2), P. 1237 - 1250

Published: Jan. 15, 2025

Incidences of infections with Severe Acute Respiratory Syndrome Coronavirus type 2 (SARS-CoV-2) are still high and treatment guidelines lack specific recommendations for outpatients Coronavirus-induced disease 2019 (COVID-19). Phytomedicine ELOM-080, an enhancer mucociliary clearance (MCC), showed benefits as add-on therapy in hospitalised COVID-19 patients. This randomised, double-blind, placebo-controlled proof-of-concept study investigated whether mild to moderate acute symptomatic would benefit from a 14-day ELOM-080 regard potential early effects on cough further typical symptoms. Outpatients documented symptom severity count coughs daily basis. Investigators safety during the visits. missed its primary objective, which was reduction coughing fits comparison placebo treatment. In analysis, no relevant differences were observed between arms. Data all randomised patients broad heterogeneity in, e.g., time courses fits, affected both magnitude timing changes baseline. However, post hoc analyses population suspected dysfunctional MCC revealed that significantly benefitted terms (p = 0.0070), difficulty breathing exertion 0.0252), earlier remission symptoms by 1-3 days. We have shown ELOM-080. These results might be clinical importance, up now has obtained market approval COVID-19. EudraCT number: 2022-003478-22.

Language: Английский

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Exploring the fumigant potential of Artemisia subg. Seriphidium essential oils and their dominant constituents against the red imported fire ants Solenopsis invicta

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120603 - 120603

Published: Feb. 4, 2025

Language: Английский

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GC/MS Analysis, Cytotoxicity, and Antimicrobial Properties of Six Moroccan Essential Oils Traditionally Used for COVID-19 Prevention

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

The COVID-19 pandemic has reignited interest in traditional medicinal plants as potential therapeutic agents. This study examines the chemical composition, cytotoxicity, and antimicrobial activity of essential oils from six Moroccan plants: Eucalyptus globulus, Artemisia absinthium, Syzygium aromaticum, Thymus vulgaris, alba, Santolina chamaecyparissus, commonly used by population for prevention. composition each oil was determined using gas chromatography-mass spectrometry (GC-MS) to identify key compounds. Cytotoxicity evaluated on Vero E6 cell line, frequently SARS-CoV-2 research, neutral red assay, with concentrations ranging 25 100 µg/mL. Antimicrobial tested against multidrug-resistant strains such Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, Bacillus subtilis disc diffusion method. GC-MS analysis revealed significant components spathulenol (15%) caryophyllene oxide (7.67%) Eucalyptus eugenol (54.96%) Syzygium aromaticum. assays indicated that higher significantly reduced viability, Thymus vulgaris showing highest IC₅₀ (8.324 µM) Artemisia absinthium lowest (18.49 µM). In terms activity, globulus exhibited strongest effect a 20 ± 0.00 mm inhibition zone subtilis, while both aromaticum herba-alba showed 12.25 0.1 same strain. These findings suggest these have potential, particularly combating resistance cytotoxic effects viral lines. Further research is necessary explore their mechanisms action ensure safety use.

Language: Английский

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Investigating Eucalyptus essential oil: classification and prediction of volatile compounds using GC-FID and FT-MIR spectroscopy combined with chemometric techniques

Vegetos, Journal Year: 2024, Volume and Issue: 37(2), P. 683 - 694

Published: Jan. 23, 2024

Language: Английский

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Natural Product-Based Screening for Lead Compounds Targeting SARS CoV-2 Mpro

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(5), P. 767 - 767

Published: May 19, 2023

Drugs that cure COVID-19 have been marketed; however, this disease continues to ravage the world without becoming extinct, and thus, drug discoveries are still relevant. Since M

Language: Английский

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Rosmarinus officinalis L. Essential Oils Impact on the Microbiological and Oxidative Stability of Sarshir (Kaymak)

Molecules, Journal Year: 2023, Volume and Issue: 28(10), P. 4206 - 4206

Published: May 20, 2023

This study investigated the effect of Rosmarinus officinalis L. essential oil, REO (one, two and three percent) on microbiological oxidative stability Sarshir during 20 days refrigerated storage (4 °C). Initially, chemical composition (gas chromatography/mass spectrometry, GC/MS), antimicrobial (paper disc diffusion) antioxidant (DPPH) properties were evaluated. Then, microbial safety, (peroxide anisidine values) overall acceptability product after addition to subsequent period determined. According GC/MS analysis, major components α-pinene (24.6%), 1,8-cineole (14.1%), camphor (13.5%), camphene (8.1%) limonene (6.1%), respectively. Moreover, it was also found that Limosilactobacillus fermentum (inhibition zone (IZ) 23.5 mm) Salmonella Typhi (IZ 16.4 most sensitive resistant spoilage pathogenic bacteria against REO, In addition, half-maximal inhibitory concentration (IC50) measured at 24.8 mg/mL, while IC50 value butylated hydroxytoluene (BHT) 16.6 mg/mL. The highest lowest bacterial populations detected in control sample containing 3% had extent lipid oxidation, peroxide values REO.

Language: Английский

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Lignin Nanoparticles with Entrapped Thymus spp. Essential Oils for the Control of Wood-Rot Fungi

Polymers, Journal Year: 2023, Volume and Issue: 15(12), P. 2713 - 2713

Published: June 17, 2023

After decades of utilization fossil-based and environmentally hazardous compounds for wood preservation against fungal attack, there is a strong need to substitute those with bio-based bioactive solutions, such as essential oils. In this work, lignin nanoparticles containing four oils from thyme species (Thymus capitatus, Coridothymus T. vulgaris, vulgaris Demeter) were applied biocides in vitro experiments test their anti-fungal effect two white-rot fungi (Trametes versicolor Pleurotus ostreatus) brown-rot (Poria monticola Gloeophyllum trabeum). Entrapment provided delayed release over time frame 7 days the carrier matrix resulted lower minimum inhibitory concentrations (0.30-0.60 mg/mL), while fungi, identical determined compared free (0.05-0.30 mg/mL). Fourier Transform infrared (FTIR) spectroscopy was used assess cell wall changes presence growth medium. The results regarding present promising approach more effective sustainable class wood-rot fungi. case nanoparticles, delivery vehicles, still optimization efficacy.

Language: Английский

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