Novel lytic bacteriophage AhFM11 as an effective therapy against hypervirulent Aeromonas hydrophila

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 23, 2024

Several farmed fish species, including carps, tilapia, salmon, and catfish, have experienced significant economic losses in aquaculture due to motile Aeromonas septicemia caused by hydrophila. In the present study, a novel lytic bacteriophage infecting hypervirulent hydrophila (vAh) was isolated characterized. This is first report of phage against vAh. Phage AhFM11 demonstrated activity both vAh strains A. reference strain ATCC 35654. The genome sequenced assembled, comprising 168,243 bp with an average G + C content 41.5%. did not harbor any antibiotic resistance genes. Genomic information along transmission electron microscopy revealed that belongs Straboviridae family. Therapeutic application monophage showed 100% survival injection, 95% immersion 93% oral feeding top-coated feed. Fish chicken meat spiked reduction These findings support can be used as biocontrol agent alternative antibiotics.

Language: Английский

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Dietary microalgal-fabricated selenium nanoparticles improve Nile tilapia biochemical indices, immune-related gene expression, and intestinal immunity

BMC Veterinary Research, Journal Year: 2024, Volume and Issue: 20(1)

Published: March 18, 2024

Abstract Background Feed supplements, including essential trace elements are believed to play an important role in augmenting fish immune response. In this context, selenium nanoparticles (SeNPs) diets via a green biosynthesis strategy have attracted considerable interest. investigation, (SeNPs, 79.26 nm) synthesized from the microalga Pediastrum boryanum were incorporated into Nile tilapia explore its beneficial effects on defense and intestinal integrity, comparison with control basal containing inorganic Se source. (No. 180, 54–57 g) fed three formulated at concentrations of 0, 0.75, 1.5 mg/kg SeNPs for 8 weeks. After trial completion, tissue bioaccumulation, biochemical indices, antioxidant pro-inflammatory cytokine-related genes, histological examination analyzed. Results Our finding revealed that dietary significantly decreased ( P < 0.05) serum alkaline phosphatase (ALP), lactate dehydrogenase (LDH), cholesterol, while increasing high-density lipoproteins (HDL). The concentration muscle tissues showed dose-dependent increase. dose upregulated interleukin IL-8 ) 1 beta (IL-1β) gene transcription compared diet. Glutathione reductase GSR glutathione synthetase GSS genes both SeNPs-supplemented groups control. No apoptotic changes or cell damages observed as indicated by proliferating nuclear antigen PCNA caspase -3 expression evidenced histopathologically. supplementation positively affects mucin-producing goblet cells (GCs), particularly mg/kg. Conclusion Therefore, these results suggest Green has promising enhancing immunity maintaining their health.

Language: Английский

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Dietary BergaPur® supplementation enhanced the hematological, immune, biochemical, and antioxidative reactions of Nile tilapia, Oreochromis niloticus

Aquaculture International, Journal Year: 2025, Volume and Issue: 33(2)

Published: Jan. 2, 2025

Abstract The existing trial investigated the effects of supplementing diet Nile tilapia with BergaPur ® on hematology, immune response, biochemical factors, and antioxidative capacity. Four groups fish an average weight 10.0 ± 0.5 g received , de-oiled phospholipid complex at a rate 0 (control), 1.0, 1.5, 2.0 g/kg for 9 weeks. Sampling included blood, serum, liver samples from four end each 3 weeks total experimental period. Hematologic readings, serum biochemical, oxidative parameters were investigated. Findings significant ( P < 0.05) hematology augmentation (corpuscular Hb, packed cell volume, leukocytic, monocytic, lymphocytic counts), remarked increased proteins (albumin, globulin, total) recorded in groups. Also, AST ALT levels decreased significantly 0.05), while glucose all sampling points. Immune (phagocytosis lysozyme activity) antioxidant (SOD, GPx, CAT) supplemented groups, compared to control, MDA level obviously incorporated over control. Our findings verified positive hematological, immune, O Oreochromis niloticus suggestive concentration 2 diet.

Language: Английский

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Integrating Fish Farming into Runoff Water Harvesting Ponds (RWHP) for Sustainable Agriculture and Food Security: Farmers’ Perceptions and Opportunities in Burkina Faso

Sustainability, Journal Year: 2025, Volume and Issue: 17(3), P. 880 - 880

Published: Jan. 22, 2025

Integrated aquaculture–agriculture systems are recognized as sustainable solutions to optimize resources, support livelihoods, and enhance food security in climate-sensitive Sahelian regions. In contexts like Burkina Faso, runoff water harvesting ponds (RWHPs) improve agricultural yields during the rainy season but remain underutilized for rest of year. This study assesses feasibility integrating fish farming into these ponds. Using Waso-2 tool, structured perception interviews were conducted with 51 farmers across 17 localities. Welch ANOVA Games–Howell tests revealed, on a scale 20, that insufficiency scored 16.01 among experienced without additional access key obstacle, while pond degradation 17.69 those access. For motivations, income generation 16.24 inexperienced farmers, whereas training opportunities 17.65 ones, highlighting varying priorities strata. Farmers preferred effluents over NPK vegetables, scoring 15.99. Some favored raw immediate use, 13.91, others decanted dried sludge gradual nutrient release, 12.39. demonstrates strong farmer interest integrated RWHP systems. Enhancing retention, supplementing groundwater, providing tailored aquaculture practices, maintenance, management recommended encourage adoption.

Language: Английский

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Diseño de una boya multiparamétrica autónoma con energía fotovoltaica y comunicación remota basada en IoT para entornos de acuicultura

Revista Cientifica de Sistemas e Informatica, Journal Year: 2025, Volume and Issue: 5(1), P. e866 - e866

Published: Jan. 20, 2025

Se diseñó un prototipo de boya multiparamétrica autónoma para abordar las limitaciones tecnológicas en el monitoreo la calidad del agua ambientes acuicultura. El objetivo fue desarrollar sistema modular y sustentable que integre energía fotovoltaica comunicación inalámbrica monitorear tiempo real parámetros críticos: pH, temperatura, oxígeno disuelto conductividad eléctrica. consta módulo emisor, receptor una plataforma transmisión datos a nube. Los materiales incluyeron PLA reforzado PETG, los componentes electrónicos fueron alimentados por panel solar 20 W conectado batería 12 V 7 Ah. Durante pruebas, demostró autonomía energética 48 horas LoRa confiable con alcance 500 m línea visión directa. diseño facilita integración sensores adaptación diversas condiciones, beneficiando pequeños productores. Sin embargo, persisten desafíos como resiliencia entornos hostiles optimización condiciones adversas, lo presenta oportunidades futuras mejoras robustez escalabilidad.

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Effect of the bipolar aluminum electrocoagulation model (BAEM) on heavy metal concentration, growth, immunological response, and parasitic activity in cultured Dicentrarchus labrax in underground seawater

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742554 - 742554

Published: April 1, 2025

Language: Английский

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A systematic analysis of recirculating aquaculture systems (RAS) and biofloc technology (BFT) for white leg shrimp (Litopenaeus vannamei) in the indoor farming system

Aquacultural Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 102544 - 102544

Published: April 1, 2025

Language: Английский

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Evaluating the response of Schizothorax prenanti to ammonia nitrogen stress using computer vision and transcriptomics

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 42, P. 102829 - 102829

Published: April 21, 2025

Language: Английский

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Bactericidal Effect and Mechanism of Polyhexamethylene Biguanide (PHMB) on Pathogenic Bacteria in Marine Aquaculture

Biology, Journal Year: 2025, Volume and Issue: 14(5), P. 470 - 470

Published: April 25, 2025

Guanidine disinfectants are cationic polymers recognized for their effective sterilization properties and ability to prevent bacterial resistance. As a result, they widely utilized in medical, healthcare, household, animal husbandry settings. However, the bactericidal effects mechanisms of guanidine marine aquaculture systems remain unclear due polymeric nature ions complexity environments. The inhibitory polyhexamethylene biguanide (PHMB) on key pathogens probiotics examined this study. It was shown that PHMB had Vibrio parahaemolyticus (VP), Photobacterium damselae subsp. (PDD), Bacillus subtilis (BS), Escherichia coli (EPEC), Staphylococcus aureus (SAU), with minimum concentrations (MICs) ranging from 3.91 125.0 µg/mL, (MBCs) 15.63 250.0 µg/mL. A stronger effect bacteria compared EPEC SAU exhibited. ion interference experiments addition calcium reduced effectiveness against VP PDD by 87.73% 53.35%, respectively. At concentration 62.50 minor changes cell surface potential energy (CSPE) were exhibited Gram-positive (SAU BS), while more significant alterations Gram-negative pathogens. revealed propidium iodide staining scanning electron microscopy (SEM) analysis membrane directly disrupted PHMB. DNA RNA release further following treatment, permeability pathogens, increase extracellular content as increased. No such observed bacteria. Additional evidence provided findings effectively inhibits mariculture systems, significantly than These results indicated could serve new antimicrobial agent mariculture.

Language: Английский

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Exploring the Multifaceted Potential of Endangered Sturgeon: Caviar, Meat and By-Product Benefits

Animals, Journal Year: 2024, Volume and Issue: 14(16), P. 2425 - 2425

Published: Aug. 21, 2024

Sturgeons are facing critical endangerment due to overfishing, habitat destruction, pollution and climate change. Their roe, highly prized as caviar, has driven the overexploitation, severely depleting wild populations. In recent years sturgeon aquaculture experienced significant growth, primarily aimed at providing high-quality caviar secondarily meat. This sector generates quantities of by-products, which mainly treated waste, being mostly discarded, impacting environment, even though they a source bioactive molecules potential applications in various sectors. article presents review proximate composition nutritional value meat, also exploring with an emphasis on processing these components, chemical functional properties. Although by-products valuable both nutritionally economically, adopting sustainable practices innovative approaches is crucial ensuring industry’s future growth maintaining ecological balance. Despite some limitations, like deficient standardization methods for extracting processing, have tremendous increase overall promote zero-waste approach, contributing achieving Sustainable Development Goals adopted by all United Nations Member States 2015.

Language: Английский

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