Recent updates on stabilization of recombinant enzymes via nanotechnology-based approaches DOI Creative Commons
Shakeel Ahmed Ansari,

Jakleen Abujamai,

Asim Muhammad Alshanberi

et al.

Discover Food, Journal Year: 2024, Volume and Issue: 4(1)

Published: Nov. 23, 2024

The food and pharmaceutical industries dominate the global enzyme market, industrial demand for enzymes is continually rising. However, low yields, poor reproducibility, occasionally subpar performance of these biocatalysts in their native form continue to restrict use enzymes. These limitations can be effectively addressed by recombinant DNA technology protein engineering, which enables high-volume production newly redesigned with enhanced characteristics that satisfy needs. Such find extensive application food, dairy, bakery, brewing, wine emulsifier/sweetener industries. Nanoparticles further advanced due distinct optical, chemical, physical electrical characteristics. Nanotechnology based approaches promoted substantial immobilization yield as compared planar 2-D surfaces greater surface area volume ratio. This offered stable catalytic activity under a variety operating conditions, sensitivity, response scale reusability rates. review highlights state‐of‐the‐art properties industrially important

Language: Английский

In Vitro Culture, Genetic Transformation and the Production of Biopharmaceuticals in Microalgae DOI Open Access
Aneta Gerszberg, Ludmiła Kolek, Katarzyna Hnatuszko-Konka

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3890 - 3890

Published: April 20, 2025

Microalgae represent a promising platform for the synthesis of recombinant proteins, particularly in context biopharmaceutical applications. Their unique combination eukaryotic cellular machinery and prokaryotic-like simplicity offers several advantages, including ability to perform complex post-translational modifications, rapid growth rates, cost-effective culture conditions. Advances genome sequencing, genetic engineering tools, omics technologies have significantly enhanced feasibility efficiency using microalgae therapeutic protein production. These advancements, coupled with development well-established transformation methods optimized vectors, enabled successful expression various biopharmaceuticals, ranging from vaccines enzymes. Here, main stages current status production exogenic proteins dedicated human therapy are presented.

Language: Английский

Citations

0
Recent updates on stabilization of recombinant enzymes via nanotechnology-based approaches DOI Creative Commons
Shakeel Ahmed Ansari,

Jakleen Abujamai,

Asim Muhammad Alshanberi

et al.

Discover Food, Journal Year: 2024, Volume and Issue: 4(1)

Published: Nov. 23, 2024

The food and pharmaceutical industries dominate the global enzyme market, industrial demand for enzymes is continually rising. However, low yields, poor reproducibility, occasionally subpar performance of these biocatalysts in their native form continue to restrict use enzymes. These limitations can be effectively addressed by recombinant DNA technology protein engineering, which enables high-volume production newly redesigned with enhanced characteristics that satisfy needs. Such find extensive application food, dairy, bakery, brewing, wine emulsifier/sweetener industries. Nanoparticles further advanced due distinct optical, chemical, physical electrical characteristics. Nanotechnology based approaches promoted substantial immobilization yield as compared planar 2-D surfaces greater surface area volume ratio. This offered stable catalytic activity under a variety operating conditions, sensitivity, response scale reusability rates. review highlights state‐of‐the‐art properties industrially important

Language: Английский

Citations

1