Xanthomonas rydalmerenesissp. nov., a novel plant bacteria isolated fromFragaria x ananassa DOI Creative Commons
Daniel J. E. McKnight, Johanna Wong‐Bajracharya, Efenaide B. Okoh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 13, 2023

ABSTRACT We describe five bacterial isolates that were isolated from Fragaria x ananassa in 1976 Rydalmere, Australia, during routine biosecurity surveillance. Initially, biochemical characterisation identified these as members of the Xanthomonas genus. To determine their species, we conducted further analysis using both phenotypic and genotypic approaches. Phenotypic involved MALDI-TOF MS BIOLOG GEN III microplates, which confirmed belonged to genus but could not classify species. Genome relatedness indices extensive phylogenetic represented a new Based on absence virulence factors typically found spp. genomes, suggest are non-pathogenic. This conclusion was supported by pathogenicity assay. findings, propose name rydalmerenesis , with DAR34855 = ICMP24941 type strain.

Language: Английский

Notification of changes in taxonomic opinion previously published outside the IJSEM. List of Changes in Taxonomic Opinion no. 41 DOI
Aharon Oren, Markus Göker

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2025, Volume and Issue: 75(1)

Published: Jan. 31, 2025

Language: Английский

Citations

0
Pangenome insights into the diversification and disease specificity of worldwide Xanthomonas outbreaks DOI Creative Commons

Viplav Agarwal,

Rachel Stubits,

Zain Nassrullah

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: July 5, 2023

The bacterial genus Xanthomonas is responsible for disease outbreaks in several hundred plant species, many of them economically important crops. In the era next-generation sequencing, thousands strains from this have now been sequenced as part isolated studies that focus on outbreak characterization, host range, diversity, and virulence factor identification. However, these data not synthesized we lack a comprehensive phylogeny genus, with some species designations public databases still relying phenotypic similarities representative sequence typing. extent genetic cohesiveness among strains, distribution factors across impact evolutionary history range are also poorly understood. study, present pangenome analysis 1,910 diverse genomes, highlighting their relationships, virulence-associated genes rates horizontal gene transfer. We find number broadly conserved classes considerable diversity Type 3 Secretion Systems (T3SSs) Secreted Effector (T3SE) repertoires different species. use to re-assign incorrectly classified phylogenetically informed evidence both monophyletic specificity convergent evolution distant same host. Finally, explore role recombination maintaining cohesion within result ancestral recent events. Understanding relationship key host-specificity provides valuable insight into mechanisms through which shift between hosts will enable us develop more robust resistance strategies against highly virulent pathogens.

Language: Английский

Citations

6
Xanthomonas rydalmerensis sp. nov., a non-pathogenic member of Group 1 Xanthomonas DOI
Daniel J. E. McKnight, Johanna Wong‐Bajracharya, Efenaide B. Okoh

et al.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2024, Volume and Issue: 74(3)

Published: March 27, 2024

Five bacterial isolates were isolated from Fragaria × ananassa in 1976 Rydalmere, Australia, during routine biosecurity surveillance. Initially, the results of biochemical characterisation indicated that these represented members genus Xanthomonas . To determine their species, further analysis was conducted using both phenotypic and genotypic approaches. Phenotypic involved MALDI-TOF MS BIOLOG GEN III microplates, which confirmed but did not allow them to be classified with respect species. Genome relatedness indices extensive phylogenetic a novel On basis minimal presence virulence-associated factors typically found genomes , we suggest are non-pathogenic. This conclusion supported by pathogenicity assay. findings, propose name rydalmerensis DAR 34855 T = ICMP 24941 as type strain.

Language: Английский

Citations

2
Celebrating the 20th anniversary of the first Xanthomonas genome sequences – how genomics revolutionized taxonomy, provided insight into the emergence of pathogenic bacteria, enabled new fundamental discoveries and helped developing novel control measures – a perspective from the French network on Xanthomonads DOI Creative Commons
Ralf Koebnik, Sophie Cesbron, Nicolas W. G. Chen

et al.

Peer Community Journal, Journal Year: 2024, Volume and Issue: 4

Published: Feb. 27, 2024

In this Opinion paper, members of the French Network on Xanthomonads give their personal view what they consider to be some groundbreaking discoveries in field molecular plant pathology over past 20 years. By celebrating 20th anniversary first Xanthomonas genome sequences, explain how genomics revolutionized taxonomy, provided insight into emergence pathogenic bacteria, enabled new fundamental and contributed development novel control measures. Collectively, such new, genomics-enabled perspective will help ensure sustainable agriculture conservation our environment future.

Language: Английский

Citations

1
Taxonogenomic analysis of the Xanthomonas translucens complex leads to the descriptions of Xanthomonas cerealis sp. nov. and Xanthomonas graminis sp. nov. DOI
James T. Tambong,

Renlin Xu,

María Constanza Fleitas

et al.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2024, Volume and Issue: 74(9)

Published: Sept. 19, 2024

The pathovar-based taxonomy of the Xanthomonas translucens group is very confusing due to an overlap plant host ranges and level specificity. Here, whole-genome sequence-based parameters (digital DNA–DNA hybridization blast -based average nucleotide identity), phylogenomic, biochemical phenotypical data were used taxonomically analyse 11 known pathovars X. complex. This polyphasic approach assigned complex into three distinct species, two which are new: , cerealis sp. nov. graminis consists pathovars: pv. (=pv. hordei ), pistaciae strain A ICMP 16316 PT undulosa secalis ). encompasses LMG 679 B 16317 with genome similarity 92.7% (dDDH) 99.0% (ANIb) suggesting similar genotypes. other new nov., remaining five designated (pv. arrhenatheri, poae phleipratensis phlei ) highly variable dDDH ANIb values ranging from 74.5 93.0% 96.7 99.2%, respectively, indication a divergent taxonomic group. Only strains pvs. showed highest genomic similarities 99.2% (ANIb), synonymic as both infect same hosts. ANI corroborated by phylogenomics clustering. fatty acid contents but type exhibited 20% less C 15 : 0 iso 40% more 17 acids than species. Based on phenotypic, sequence data, we propose T (=NCPPB 1944 726 2700 respectively.

Language: Английский

Citations

1
Diseases of Pearl Millet DOI
Christopher R. Little,

Ramasamy Perumal,

T. C. Todd

et al.

Agronomy monograph/Agronomy, Journal Year: 2024, Volume and Issue: unknown, P. 299 - 391

Published: Sept. 18, 2024

Language: Английский

Citations

1
Xanthomonas rydalmerenesissp. nov., a novel plant bacteria isolated fromFragaria x ananassa DOI Creative Commons
Daniel J. E. McKnight, Johanna Wong‐Bajracharya, Efenaide B. Okoh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 13, 2023

ABSTRACT We describe five bacterial isolates that were isolated from Fragaria x ananassa in 1976 Rydalmere, Australia, during routine biosecurity surveillance. Initially, biochemical characterisation identified these as members of the Xanthomonas genus. To determine their species, we conducted further analysis using both phenotypic and genotypic approaches. Phenotypic involved MALDI-TOF MS BIOLOG GEN III microplates, which confirmed belonged to genus but could not classify species. Genome relatedness indices extensive phylogenetic represented a new Based on absence virulence factors typically found spp. genomes, suggest are non-pathogenic. This conclusion was supported by pathogenicity assay. findings, propose name rydalmerenesis , with DAR34855 = ICMP24941 type strain.

Language: Английский

Citations

0