The human gut fungiome: Role in physiology and detoxification DOI Open Access

Seralini Gilles-Eric

Journal of Food Science and Nutrition Therapy, Journal Year: 2023, Volume and Issue: 9(1), P. 001 - 007

Published: May 2, 2023

The intestinal microbiota is an ecosystem where bacteria, archaea, viruses, and protists, are entangled, but not alone. We take as the bacterial community because, in many historical papers, probes to detect other organisms intestine were rarely used. But addition, unicellular fungi or yeasts do exist microbiota, their assembly called mycome fungiome, it can reach size number of our human cells. bacteria far more numerous. For a long time, whole was taken for nest pathogens, fact, adapted evolved symbiotic cells helping only digestion, also angiogenesis, tissue growth, vitamin synthesis, nervous, endocrine, immune systems even detoxification system. Much what we swallow will become fermented, like bread, cheese, wine. Since beginning agriculture, food eaten lightly by including first plants treated pesticides. Natural still unknown hundreds types species at very least, bringing particular during work fermentation multiplicity aromas that play role famous detoxification; usual diet provides few strains these yeasts. In all cells, ubiquitous cytochrome P450 detoxifying enzymes form vast family involved cell respiration, which stimulated some extent signals secreted. Yeasts could attract coordinate eliminate part chemical pollutants.

Language: Английский

Cinnamon nanoemulsion mitigates acetamiprid-induced hepatic and renal toxicity in rats: biochemical, histopathological, immunohistochemical, and molecular docking analysis DOI Creative Commons
Ahmed A. A. Aioub, Sameh A. Abdelnour, Ahmed S. Hashem

et al.

BMC Veterinary Research, Journal Year: 2024, Volume and Issue: 20(1)

Published: June 12, 2024

Acetamiprid (ACDP) is a widely used neonicotinoid insecticide that popular for its efficacy in controlling fleas domestic settings and pets. Our study aims to offer comprehensive examination of the toxicological impacts ACDP prophylactic effects cinnamon nanoemulsions (CMNEs) on pathological, immunohistochemical, hematological analyses induced by taking twice ‎week 28 days. Forty healthy rats were divided into four groups (n = 10) at random; first group served as control rats; second received CMNEs (2 mg/Kg body weight); third acetamiprid (ACDP group; 21.7 weight), fourth was given both oral gavage. Following period, tissue blood samples extracted prepared analysis. According GC-MS analysis, had several bioactive ingredients protected liver from oxidative stress upregulating antioxidant anti-inflammatory agents. findings demonstrated whereas treatment considerably boosted white cells (WBCs) lymphocytes, it significantly lowered weight gain (BWG), red (RBCs), hemoglobin (Hb), hematocrit (HCT), platelets (PLT). notably reduced enzyme activities: superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) elevated hydrogen peroxide malondialdehyde levels compared with other groups. remarkably raised alanine aminotransferase (ALT), aspartate amino transaminase (AST), alkaline phosphatase (ALP) levels.Moreover, histopathological immunohistochemistry assays discovered severe toxic effect kidney following delivery. Furthermore, cyclooxygenase 2 (COX-2) + immunoexpression enhanced after CMNEs. All parameters above returned nearly normal coadministration The molecular docking cinnamaldehyde COX-2 also confirmed protective potential against toxicity. highlighted along diminished toxicity cutting down enhancing capacity, demonstrating effectiveness lessening

Language: Английский

Citations

5
Molecular insights into the antioxidative and anti-inflammatory effects of P-coumaric acid against bisphenol A-induced testicular injury: In vivo and in silico studies DOI
Samet Tekin, Emin Şengül, Serkan Yıldırım

et al.

Reproductive Toxicology, Journal Year: 2024, Volume and Issue: 125, P. 108579 - 108579

Published: March 19, 2024

Language: Английский

Citations

4
Toxicity studies of condensed fuzheng extract in mice and rats DOI Creative Commons

J. T. Wang,

Li Wang, Junzhen Tan

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(3), P. e24780 - e24780

Published: Jan. 19, 2024

Nutritional supplements have been used to improve immune function. Condensed fuzheng extract (CFE) is a well-known traditional Chinese medicine (TCM) formula that predominantly made from sheep placenta, Astragalus mongholicus Bunge, and Polygonatum kingianum Collett & Hemsl. However, the toxicological profile of CFE has not determined. In this study, we investigated acute (14 days) sub-chronic (90 oral toxicities in mice rats phytochemical composition CFE.Materials methodsFor assessment toxicity, 80 ICR both sexes were randomly divided into four groups. Three groups treated with 4500, 2250 1125 mg/kg/d bw daily (n = 10/group per sex) for 14 days; separate group was as control. To test male female Sprague Dawley orally administered 8150, 4075 or 2037 mg/kg 90 control included. Hematological, biochemical, histopathological markers tested at end experiment. The chemical determined by UPLC-HRMS method.ResultsIn toxicity studies, no mortalities, indications abnormality, treatment-related adverse effects observed. LD50 higher than 4500 mg/kg. There significant changes hematological biochemical data treatment compared (p > 0.05). Histopathological analyses heart, liver, spleen, lungs, kidneys, thymus, testes (male rats) ovaries (female revealed anatomical each organ. Phytochemical analysis presence flavonoids (highest abundance), phenols alkaloids. conclusion, our results showed safe non-toxic formula. We also reported phytochemicals may possess important pharmacological effects.

Language: Английский

Citations

2
Structural gonadal lesions observed in Japanese quail (Coturnix coturnix japonica) following exposure during puberty to the neonicotinoid pesticide, imidacloprid DOI Creative Commons
Mohammed I.A. Ibrahim, Antoinette V. Lensink,

Rephima M. Phaswane

et al.

Tissue and Cell, Journal Year: 2024, Volume and Issue: 89, P. 102450 - 102450

Published: June 19, 2024

Exposure to the neonicotinoid insecticide, imidacloprid (IMI), causes reproductive toxicity in mammals and reptiles. However, reports on effects of IMI gonads birds are grossly lacking. Therefore, this study investigated pubertal exposure histology, ultrastructure, as well cytoskeletal proteins, desmin, smooth muscle actin vimentin, Japanese quail (Coturnix coturnix japonica). Quails were randomly divided into four groups at 5 weeks age. The control group was given only distilled water, whereas, other three experimental groups, administered by oral gavage 1.55, 3.1, 6.2 mg/kg, twice per week for 4 weeks. doses 3.1 mg/kg caused dose-dependent histopathological changes ovary testis. In ovary, accumulation lymphocytes, degenerative changes, necrosis with granulocyte infiltrations observed, while testis, distorted seminiferous tubules, germ cell sloughing, vacuolisations, apoptotic bodies, autophagosomes, mitochondrial damage detected. These accompanied a decreased number primary follicles (P ≤ 0.05) decrease epithelial height, luminal, tubular diameters tubules two higher dosages. addition, had negative effect immunostaining intensity actin, vimentin ovarian testicular tissue. conclusion, during puberty can lead range alterations quails, which may ultimately result infertility.

Language: Английский

Citations

1
Long Term Administration of <i>Lannea acida</i> Rich. (Anacardiaceae) Reverses the Imidacloprid-Induced Fertility Impairments in Adult Male Rat through Androgenic and Antioxidant Properties DOI Open Access
Aimé Césaire Momo Tetsatsi,

Gladis Komguep Djuidje,

Prechmy Carole Ngueyong Nsamou

et al.

Journal of Biosciences and Medicines, Journal Year: 2024, Volume and Issue: 12(03), P. 62 - 76

Published: Jan. 1, 2024

Aim: The harmful effects of pesticides have been largely documented in recent times. But effective therapeutic solutions to pesticide related male infertility are yet be established. This study investigated the curative Lannea acida on imidacloprid (IMI)-induced hypofertility Wistar rats. Methods: Rats 150 – 200 g were administered IMI (22.5 mg/kg) for two weeks and partitioned into control (distilled water, vitamin E, clomiphene citrate) or test (aqueous (340 mg/kg), methanol (170 extract) groups eight treatment. Animals sacrificed at end treatment samples collected sperm, antioxidant hormonal analysis. Fertility tests performed from day 47 fertility indices estimation. Results expressed as mean ± SEM one way ANOVA was applied using STATISTICA Software. Results: Exposition resulted a significant decrease sperm count, motility, viability normality, testosterone LH, coupled an increase oxidative stress markers. Moreover, impaired evidenced by drop index litter size. Similar citrate plant extracts significantly improved parameters, sexual hormones decreased More importantly, size restored, especially with aqueous extract. Conclusion: Present results indicate that L. possesses potentials against IMI-induced through its androgenic properties. However, extract spermatozoa DNA structure offsprings exposed parents studied conclude total recovery toxicity.

Language: Английский

Citations

0
Crosstalk between Tlr4/Nf-Κb, Nrf2-Keap1, and Jak-Stat Signaling Pathways: Possible Mechanism for Ace-Induced Immunotoxicity In Zebrafish Larvae DOI
Guanghua Mao, Dan Li,

Yacan Gong

et al.

Published: Jan. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Language: Английский

Citations

0
Transcriptome analysis reveals the molecular mechanisms of neonicotinoid acetamiprid in Leydig cells DOI
Yang Liu, Ce Wang, Yue Ma

et al.

Toxicology and Industrial Health, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

At present, the reproductive toxicology of neonicotinoids has received greater attention, however, its potential mechanisms are still not fully understood. Acetamiprid (ACE) is a new-generation neonicotinoid and become ubiquitous contaminant in environment. This study aimed to investigate toxic effects ACE TM3 Leydig cells based on transcriptome analysis. The viability apoptosis exposed different concentrations were assessed by CCK8 flow cytometry, respectively. After exposure, analysis was performed screen differential expression genes (DEGs), followed qPCR verification. Results showed that exposure resulted time- dose-dependent decrease (

Language: Английский

Citations

0
6‐Chloronicotinic Acid Induces Toxicity in Mouse Neural Stem Cells via the C3ar1 Signaling DOI Open Access
Min He,

Yahang Lin,

Xiaojing Zhang

et al.

Journal of Applied Toxicology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 29, 2024

ABSTRACT Neural stem cells (NSCs) are essential for brain development due to their ability proliferate and differentiate into various neural cell types. Neonicotinoid insecticides (NNIs), which have replaced traditional pesticides, now widely used frequently detected in environmental biological samples. Prenatal exposure NNIs has been associated with an increased risk of neurodevelopmental disorders offspring, yet the causal relationship underpinning mechanism remain be clarified. As one primary metabolites chloropyridinyl neonicotinoids, 6‐chloronicotinic acid (6‐ClNA) identified as a potential neurotoxin, though its effects on NSCs not fully explored. Here, we demonstrate that 6‐ClNA significantly disrupted NSC proliferation differentiation vitro. Transcriptomic analyses revealed altered expression pathways related proliferation, apoptosis, inflammation, notable activation C3ar1/C1qa signaling axis. Genetic ablation C3ar1 using siRNA markedly restored neurosphere formation, well reduced suggesting central role mediating 6‐ClNA's neurotoxic effects. These findings imply early‐life may affect fitness function NSCs, wherein pathway plays indispensable role.

Language: Английский

Citations

0
Benchmark Dose Approach to DNA and Liver Damage by Chlorpyrifos and Imidacloprid in Male Rats: The Protective Effect of a Clove-Oil-Based Nanoemulsion Loaded with Pomegranate Peel Extract DOI Creative Commons

Alia Ahmed Abdel-Hamid Omar,

Marwa Gad, Amel A. Refaie

et al.

Toxics, Journal Year: 2023, Volume and Issue: 11(7), P. 569 - 569

Published: June 30, 2023

Pesticides are widely used around the world to increase crop production. They also have negative impacts on animals, humans, and ecosystem. This is first report evaluating a novel pomegranate-extract-loaded clove-oil-based nanoemulsion (PELCN) its potential for reducing oxidative stress DNA damage, as well hepatoprotective effects against imidacloprid (IM) chlorpyrifos (CPF) toxicity in male rats. The benchmark dose (BMD) approach was study dose–response of IM CPF. CPF were administered daily 28 days at doses 14, 28, 54 mg/kg body weight (bw) 1, 2, 4 bw via drinking water. PELCN orally 50 bw/day pomegranate extract, 500 clove oil nanoemulsion, or high In rats, caused reduction gain hepatotoxic evidenced by increases liver enzymes AST, ALT, ALP. damage rats indicated decreased activity GST, GPX, SOD, CAT serum TAC. produced significant dose-dependent hepatocyte cells, resulting moderate severe with cells that more inflammatory enlarged sinusoids compacted nuclei. had higher BMD than both weight, suggesting dose-dependently toxic IM. Albumin highly sensitive biomarker IM, while total protein CPF-treated GPx an extremely treatment, parameters Therefore, comparable doses, has cause hepatotoxicity can be mitigated administering containing extract. acts protector these insecticides, especially doses. based bioavailability stability which antioxidant properties.

Language: Английский

Citations

1
The human gut fungiome: Role in physiology and detoxification DOI Open Access

Seralini Gilles-Eric

Journal of Food Science and Nutrition Therapy, Journal Year: 2023, Volume and Issue: 9(1), P. 001 - 007

Published: May 2, 2023

The intestinal microbiota is an ecosystem where bacteria, archaea, viruses, and protists, are entangled, but not alone. We take as the bacterial community because, in many historical papers, probes to detect other organisms intestine were rarely used. But addition, unicellular fungi or yeasts do exist microbiota, their assembly called mycome fungiome, it can reach size number of our human cells. bacteria far more numerous. For a long time, whole was taken for nest pathogens, fact, adapted evolved symbiotic cells helping only digestion, also angiogenesis, tissue growth, vitamin synthesis, nervous, endocrine, immune systems even detoxification system. Much what we swallow will become fermented, like bread, cheese, wine. Since beginning agriculture, food eaten lightly by including first plants treated pesticides. Natural still unknown hundreds types species at very least, bringing particular during work fermentation multiplicity aromas that play role famous detoxification; usual diet provides few strains these yeasts. In all cells, ubiquitous cytochrome P450 detoxifying enzymes form vast family involved cell respiration, which stimulated some extent signals secreted. Yeasts could attract coordinate eliminate part chemical pollutants.

Language: Английский

Citations

0