Identification and Characterization of Glutathione S-transferase Genes in Spodoptera frugiperda (Lepidoptera: Noctuidae) under Insecticides Stress

Toxics, Journal Year: 2023, Volume and Issue: 11(6), P. 542 - 542

Published: June 19, 2023

Insect glutathione S-transferases (GSTs) serve critical roles in insecticides and other forms of xenobiotic chemical detoxification. The fall armyworm, Spodoptera frugiperda (J. E. Smith), is a major agricultural pest several countries, especially Egypt. This the first study to identify characterize GST genes S. under insecticidal stress. present work evaluated toxicity emamectin benzoate (EBZ) chlorantraniliprole (CHP) against third-instar larvae using leaf disk method. LC50 values EBZ CHP were 0.029 1.250 mg/L after 24 h exposure. Moreover, we identified 31 genes, including 28 cytosolic 3 microsomal SfGSTs from transcriptome analysis genome data frugiperda. Depending on phylogenetic analysis, sfGSTs divided into six classes (delta, epsilon, omega, sigma, theta, microsomal). Furthermore, investigated mRNA levels qRT-PCR stress Interestingly, SfGSTe10 SfGSTe13 stood out with highest expression treatments. Finally, molecular docking model was constructed between most upregulated (SfGSTe10 SfGSTe13) least (SfGSTs1 SfGSTe2) larvae. showed have high binding affinity SfGSTe10, energy -24.41 -26.72 kcal/mol, respectively, sfGSTe13, -26.85 -26.78 respectively. Our findings are important for understanding role GSTs regarding detoxification processes CHP.

Language: Английский

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Intergenerational effect of cyproflanilide at sublethal level to Chilo suppressalis (Walker) (Lepidoptera: Pyralidae)

Crop Protection, Journal Year: 2025, Volume and Issue: unknown, P. 107184 - 107184

Published: March 1, 2025

Language: Английский

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Resistance and fitness costs of the Helicoverpa armigera after selection with the tetraniliprole newly developed diamide insecticide

Crop Protection, Journal Year: 2024, Volume and Issue: 179, P. 106622 - 106622

Published: Feb. 12, 2024

Language: Английский

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First report of resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to lambda-cyhalothrin from Pakistan: baseline susceptibility, selection, occurrence of cross-resistance, realized heritability, and inheritance mode of resistance

Journal of Economic Entomology, Journal Year: 2024, Volume and Issue: 117(4), P. 1636 - 1647

Published: June 27, 2024

Abstract Fall armyworm (FAW) Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a global pest causing damage to several crops. However, its management using chemical control challenge due capacity evolve resistance insecticides. After 6 generations of selection with lambda-cyhalothrin, the LC50 for insecticide-resistant strain (Lambda-Sel) was 486 ppm, higher than that field (FAW-MUL) (7.5 ppm), susceptible laboratory (Lab-PK) (0.46 and unselected (UNSEL) (5.26 ppm). Laboratory lambda-cyhalothrin increased from 16.3- 1056.52-fold 1.43- 92.4-fold compared Lab-PK UNSEL strains, respectively. The selected S. presented low cross-resistance chlorpyrifos, moderate deltamethrin indoxacarb, very spinosad, no emamectin benzoate. realized heritability (h2) in Lambda-Sel high (0.88). reciprocal cross progenies F1 (Lambda-Sel ♀ × ♂), F1ʹ ♂ ♀), BC1 (F1 BC2 ♂) showed ratios 545.64-, 396.52-, 181.18-, 146.54-fold, respectively Lab-PK. degree dominance values indicates incompletely dominant resistance. difference between observed expected mortality backcross populations (BC1 BC2) revealed polygenic In conclusion, autosomal, dominant, polygenic. These findings provide new insights insect strategies mitigate occurrence this pest.

Language: Английский

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Resistance Realized Heritability and Fitness Cost of Cyproflanilide in Rice Stem Borer, Chilo suppressalis (Lepidoptera: Pyralidae)

Agronomy, Journal Year: 2024, Volume and Issue: 14(10), P. 2249 - 2249

Published: Sept. 29, 2024

The rice stem borer (RSB) Chilo suppressalis is a devastating pest with resistance to number of insecticides. Recently, the new meta-diamide insecticide cyproflanilide has been considered an effective control RSB. However, its risk not reported. In present study, we aimed assess and evaluate fitness cost after RSB was exposed cyproflanilide. After five generations selection, level increased by 1.5-fold. When h2 0.125, 10-fold increase in LD50 values expected fourteen thirty-one at selection intensity 90% 50%, respectively. selected population (RSB-SEL) had significant differences developmental duration eggs, 1st, 2nd, 3rd, 6th instar larvae, female pupae compared unselected (RSB-UNSEL). Besides, adult longevity shortened, average pupal weight males, emergence rate, sex ratio, oviposition, mean fecundity, full life cycle rate were decreased RSB-SEL. intrinsic (r), net reproductive (R0), finite (λ) RSB-SEL significantly lower than those RSB-UNSEL, while generation time (T) longer that RSB-UNSEL. Based on results prediction required for LD50, potential development exists continuous excessive use These will be useful designing dose C. field.

Language: Английский

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Resistance risk assessment and fitness cost of cyproflanilide in the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae)

Crop Protection, Journal Year: 2024, Volume and Issue: 188, P. 107033 - 107033

Published: Nov. 15, 2024

Language: Английский

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Transcriptomic and biochemical insights into fall armyworm (Spodoptera frugiperda) responses on silicon-treated maize

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e16859 - e16859

Published: Feb. 23, 2024

Background The fall armyworm, Spodoptera frugiperda , is an agricultural pest of significant economic concern globally, known for its adaptability, pesticide resistance, and damage to key crops such as maize. Conventional chemical pesticides pose challenges, including the development resistance environmental pollution. study aims investigate alternative solution: application soluble silicon (Si) sources enhance plant against armyworm. Methods Silicon dioxide (SiO 2 ) potassium silicate (K SiO 3 were applied maize plants via foliar spray. Transcriptomic biochemical analyses performed gene expression changes in armyworm feeding on Si-treated Results indicated a impact expression, with large number differentially expressed genes (DEGs) identified both K treatments. Furthermore, Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis critical DEGs involved specific pathways, amino acid, carbohydrate, lipid, energy, xenobiotics metabolisms, signal transduction, posttranslational modification, significantly altered at Si sources. Biochemical further revealed that treatments inhibited several enzyme activities (glutamate dehydrogenase, trehalase, glucose-6-phosphate chitinase, juvenile hormone esterase, cyclooxygenase while simultaneously inducing others (total protein, lipopolysaccharide, fatty acid synthase, ATPase, cytochrome P450), thus suggesting toxic effect In conclusion, applications influence potentially offering sustainable management strategy.

Language: Английский

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Biochemical and molecular characterization of pyrifluquinazon resistance in Bemisia tabaci Asia I

Crop Protection, Journal Year: 2024, Volume and Issue: 185, P. 106901 - 106901

Published: Aug. 15, 2024

Language: Английский

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Toxicity and fitness evaluation of Chrysoperla carnea resistant strains on cotton mealy bug Phenacoccus solenopsis Tinsely.

Crop Protection, Journal Year: 2024, Volume and Issue: 187, P. 106943 - 106943

Published: Sept. 10, 2024

Language: Английский

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