SLC7A11 Silencing Modulates Ferroptosis and Autophagy to Reduce Sodium Arsenite-Induced Activation of Hepatic Stellate Cells

Published: June 17, 2024

Arsenic is a prevalent environmental pollutant with recognized carcinogenic properties. Liver fibrosis frequent consequence of arsenic poisoning, the activation hepatic stellate cells (HSCs) being central event. Solute Carrier Family 7 Member 11 (SLC7A11), pivotal regulator ferroptosis, may be involved in process arsenic-induced liver fibrosis. This study utilized lentiviral vector-mediated SLC7A11 silencing LX-2 (a type human cells) to establish an knockout cell model, which was then exposed sodium arsenite (NaAsO2). Protein interactions were assessed through Immunoprecipitation (IP), and protein levels evaluated via Western blot analysis. It found that NaAsO2 decreased cellular Fe2+ nuclear receptor co-activator 4 (NCOA4) expression, reversing these effects. Additionally, IP analysis revealed interaction between Beclin1 proteins NaAsO2. Silencing attenuated reduction Tumor p53(P53), p-mammalian target rapamycin (p-mTOR) levels, along rise Beclin1, Phosphorylated adenosine monophosphate activated kinase (p-AMPK), α-smooth muscle actin (α-SMA) Fibroblast protein-α (FAP) induced by Consequently, promoted reduced autophagy P53/AMPK/mTOR pathway, inhibited HSC NaAsO2, potentially mitigating

Language: Английский

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Hydrothermal carbonization of primary sewage sludge in a prototype reactor: Sensitivity analysis of key parameters for hydrochar and liquid phase upscaling and reuse

Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(6), P. 114716 - 114716

Published: Nov. 7, 2024

Language: Английский

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Relationships between microbial characteristics and the arsenic cycle in high-arsenic groundwater

Environmental Research Communications, Journal Year: 2024, Volume and Issue: 6(11), P. 115027 - 115027

Published: Nov. 1, 2024

Abstract Microbial activities play a pivotal role in the migration and transformation of arsenic (As) within groundwater systems, particularly at high concentrations. Focusing on Kuitun River Basin, this study used high-throughput sequencing to unravel intricate diversity microbial communities their influence As enrichment. Groundwater with elevated hosted richer more diverse populations compared that lower levels. The Sobs Shannon indices for high-As group initially declined before rising increasing Key taxa dominating these ecosystems included Pseudomonas , Acinetobacter Aquabacterium Novosphingobium Sphingobium showing substantial variation abundance across different Distance-based redundancy analysis emphasized importance dissolved oxygen shaping landscape, followed closely by interplay between iron Flavobacteriaceae Burkholderiaceae displayed inverse correlations levels, highlighting multifaceted relationship chemistry. This investigation underscores profound impact concentration gradients biodiversity elucidates complex regulatory framework where environmental parameters microorganisms collaborate governing accumulation groundwater. results enrich our understanding biogeochemical dynamics underlying behavior subterranean aquatic habitats.

Language: Английский

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Microbiological Activity and Oxygen Availability Influence Arsenic Mobilization and Redox Transformation in an Arsenic-Rich Aquifer

Exposure and Health, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Language: Английский

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