Analysis, levels and seasonal variation of cyanotoxins in freshwater ecosystems DOI Creative Commons
Daria Filatova, Massimo Picardo, Óscar Núñez

et al.

Trends in Environmental Analytical Chemistry, Journal Year: 2020, Volume and Issue: 26, P. e00091 - e00091

Published: May 6, 2020

Nutrient over-enrichment in freshwater environments, together with the on-going climate change, favour toxin-producing cyanobacteria bloom. Human health hazard may arise from drinking contaminated water. Additionally, cyanobacterial blooms affect other economic areas such as tourism, recreation, commercial fishery, water management and monitoring. Nowadays there is a scarcity of information on seasonal variations cyanotoxins various regions. Understanding historical trends variation patters foundation for forecasting will help to develop effective strategies. This review gives an overview cyanotoxins' analysis levels environments particular emphasis Europe. Recent analytical approaches are discussed patterns three major European zones (Mediterranean, continental, Atlantic) were distinguished. data multi-year studies showed tendency increasing levels.

Language: Английский

Determination of microcystin-LR, employing aptasensors DOI

Hasan Badie Bostan,

Seyed Mohammad Taghdisi, Jenna L. Bowen

et al.

Biosensors and Bioelectronics, Journal Year: 2018, Volume and Issue: 119, P. 110 - 118

Published: Aug. 8, 2018

Language: Английский

Citations

54
Microcystin-LR affects the hypothalamic-pituitary-inter-renal (HPI) axis in early life stages (embryos and larvae) of zebrafish DOI
Liang Chen, Yeke Wang, John P. Giesy

et al.

Environmental Pollution, Journal Year: 2018, Volume and Issue: 241, P. 540 - 548

Published: May 22, 2018

Language: Английский

Citations

50
Cyanobacteria and their secondary metabolites in three freshwater reservoirs in the United Kingdom DOI Creative Commons
Daria Filatova, Martin R. Jones,

John A. Haley

et al.

Environmental Sciences Europe, Journal Year: 2021, Volume and Issue: 33(1)

Published: March 9, 2021

Abstract Background Bloom-forming cyanobacteria occur globally in aquatic environments. They produce diverse bioactive metabolites, some of which are known to be toxic. The most studied cyanobacterial toxins microcystins, anatoxin, and cylindrospermopsin, yet more than 2000 metabolites have been identified date. Data on the occurrence cyanopeptides other microcystins surface waters sparse. Results We used a high-performance liquid chromatography–high-resolution tandem mass spectrometry/tandem spectrometry (HPLC–HRMS/MS) method analyse cyanotoxin cyanopeptide profiles raw drinking water collected from three freshwater reservoirs United Kingdom. A total 8 were quantified using reference standards. further 20 based suspect-screening procedure, with class-equivalent quantification. Samples Ingbirchworth reservoir showed highest concentrations, reaching 5.8, 61, 0.8 µg/L August, September, October, respectively. Several classes anabaenopeptins, cyanopeptolins, dominating September 37%, 36%, 26%, Tophill Low reached 2.4 but remained below 0.2 months. Embsay did not exceed 0.1 µg/L. At Low, maximum chlorophyll-a concentrations 37 22 µg/L, respectively, count 6 × 10 4 cells/mL observed at, or few days after, peak concentrations. These values World Health Organization’s guideline levels for relatively low probability adverse health effects, defined as 2 cells/mL. Conclusions This data is first present aeruginosins, microginins, along U.K. reservoirs. better understanding those that abundant can inform future monitoring studies abatement efficiency during treatment.

Language: Английский

Citations

38
Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece DOI Creative Commons
Spyros Gkelis, Manthos Panou,

Despoina Konstantinou

et al.

Toxins, Journal Year: 2019, Volume and Issue: 11(8), P. 436 - 436

Published: July 25, 2019

Cyanobacteria are a diverse group of photosynthetic Gram-negative bacteria that produce an array secondary compounds with selective bioactivity against broad spectrum organisms and cell lines. In this study, 29 strains isolated from freshwaters in Greece were classified using polyphasic approach assigned to Chroococcales, Synechococcales, Nostocales, representing 11 genera 17 taxa. There good agreements between 16S ribosomal RNA (rRNA)–cpcBA–internal genetic spacer (IGS) characterization morphological features, except for the Jaaginema–Limnothrix which appears intermixed needs further elucidation. Methanol extracts analyzed cyanotoxin production tested pathogenic species several cancer We report first time Nostoc oryzae strain rice fields capable producing microcystins (MCs) Chlorogloeopsis fritschii plankton lake, suggesting may also occur freshwater temperate habitats. Strains very high or identical rRNA gene sequences displayed different antibacterial cytotoxic activities. Extracts Synechococcus cf. nidulans showed most potent activity Staphylococcus aureus, whereas Jaaginema sp. exhibited activities human colorectal adenocarcinoma hepatocellular carcinoma cells. Thessaloniki Aristotle University Microalgae (TAU-MAC) 0110 0210 caused pronounced changes actin network triggered formation numerous lipid droplets green monkey kidney cells, oxidative stress and/or mitochondrial damage leading apoptosis.

Language: Английский

Citations

38
Suspect screening of natural toxins in surface and drinking water by high performance liquid chromatography and high-resolution mass spectrometry DOI Creative Commons
Massimo Picardo, Josep Sanchís, Óscar Núñez

et al.

Chemosphere, Journal Year: 2020, Volume and Issue: 261, P. 127888 - 127888

Published: Aug. 21, 2020

Besides anthropogenic contamination, freshwater environments can also be affected by the presence of natural toxins. Mycotoxins, plant toxins and cyanotoxins are most relevant groups that found in aquatic system. However, until now, only have been more carefully studied. In present work, single workflow for assessment waters, based on suspect screening target a selected group is presented. The approach triple-stage solid-phase extraction (SPE) able to isolate wide range different polarities, followed liquid chromatography coupled high-resolution mass spectrometry (HPLC-ddHRMS2) using Q-Exactive Orbitrap analyser. acquisition was performed full-scan (FS) data-dependant (ddMS2) mode, working under positive negative mode. For tentative identification, on-line databases such as ChemSpider MzCloud an in-house list with 2384 structures, includes cyanotoxins, mycotoxins, were used. Also, thanks MS2 data, it possible achieve high level identification confidence, but confirmation comparing standards suspected compounds. those, analytical parameters developed method validated, quantification external calibration. Validation showed recoveries between 53 95%, limits detection (MDL) 0.02 1.22 μg/L. This applied study 4 sampling sites along Ter River Catalonia (NE Spain). this preliminary 23 tentatively identified, 9 them confirmed (aflatoxin B1, anatoxin-a, nodularin, microcystin-LR, baicalein, kojic acid, cinchonine, B-asarone atropine). results these compounds concentrations below 1 μg/L all cases, considered safe according actual legislation. allows comprehensive waters.

Language: Английский

Citations

38
A Summer of Cyanobacterial Blooms in Belgian Waterbodies: Microcystin Quantification and Molecular Characterizations DOI Creative Commons
Wannes Hugo R. Van Hassel, Mirjana Andjelković, Benoit Durieu

et al.

Toxins, Journal Year: 2022, Volume and Issue: 14(1), P. 61 - 61

Published: Jan. 16, 2022

In the context of increasing occurrences toxic cyanobacterial blooms worldwide, their monitoring in Belgium is currently performed by regional environmental agencies (in two three regions) using different protocols and restricted to some selected recreational ponds lakes. Therefore, a global assessment based on comparison existing datasets not possible. For this study, 79 water samples from five lakes Wallonia occasional Flanders Brussels, including canal, were analyzed. A Liquid Chromatography with tandem mass spectrometry (LC-MS/MS) method allowed detect quantify eight microcystin congeners. The mcyE gene was detected PCR, while dominant species identified 16S RNA amplification direct sequencing. diversity for characterized amplicon Microcystins above limit quantification (LOQ) 68 samples, World Health Organization (WHO) recommended guideline value microcystins (24 µg L-1) surpassed 18 samples. concentrations ranged 0.11 L-1 2798.81 total microcystin. 45 dominance genera Microcystis sp., Dolichospermum Aphanizomenon Cyanobium/Synechococcus Planktothrix Romeria Cyanodictyon Phormidium sp. shown. Moreover, 75.71% all

Language: Английский

Citations

22
LC-MS/MS Validation and Quantification of Cyanotoxins in Algal Food Supplements from the Belgium Market and Their Molecular Origins DOI Creative Commons
Wannes Hugo R. Van Hassel,

Anne‐Catherine Ahn,

Bart Huybrechts

et al.

Toxins, Journal Year: 2022, Volume and Issue: 14(8), P. 513 - 513

Published: July 27, 2022

Food supplements are gaining popularity worldwide. However, harmful natural compounds can contaminate these products. In the case of algae-based products, presence toxin-producing cyanobacteria may cause health risks. data about prevalence algal food on Belgian market and possible contaminations with cyanotoxins scarce. Therefore, we optimized validated a method based Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry to quantify eight microcystin congeners nodularin in supplements. Our analytical was successfully applied 35 supplement samples. Nine out samples contained congeners, which three exceeded 1 µg g−1, previously proposed guideline value. Additionally, mcyE gene amplified sequenced ten products identify taxon responsible for toxin production. For seven samples, could be associated Microcystis sp. EFSA posology consumption algal-based were both combined our establish different exposure scenarios assess risks propose new values.

Language: Английский

Citations

21
Cyanobacterial blooms in earthen aquaculture ponds and their impact on fisheries and human health in Bangladesh DOI Open Access
Sunzida Sultana, Sadiqul Awal, Nowrin Akter Shaika

et al.

Aquaculture Research, Journal Year: 2022, Volume and Issue: 53(15), P. 5129 - 5141

Published: July 16, 2022

Cyanobacteria can form intense and sometimes toxic blooms in all kinds of aquatic ecosystems, typified as cyanobacterial bloom. Cyanobacterial create a range harmful toxins, which may disturb water sources, successively posing serious health threat to living organisms, at this stage they are termed algal (CyanoHABs). The occurrence toxins globally reported, so is the evident Bangladesh, mainly trigged by increased anthropogenic eutrophication global climate change. accumulation several human's drinking water, recreational biota such zooplankton, fish larvae juveniles interrupt aquaculture, ecosystem public Bangladesh. As management measures cyanobacteria cyanotoxin insufficient proper for mitigating worldwide crucial maintenance sustainable development functional some recommendations illustrated. Further appropriate research on biology ecology cyanobacteria, their relationship with environmental factors well nutrient ponds manage noxious algae essential minimize adverse impacts aquaculture country.

Language: Английский

Citations

20
Irrigation-dependent accumulation of microcystin in different crops under mid-scale greenhouse conditions DOI Creative Commons
Wannes Hugo R. Van Hassel,

Elise Tardy,

B. G. Cottyn

et al.

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101753 - 101753

Published: Feb. 1, 2025

Language: Английский

Citations

0
Health and Environmental Impacts of Cyanobacteria and Cyanotoxins from Freshwater to Seawater DOI Creative Commons

Tamara Villalobos,

Benjamín A. Suárez‐Isla,

Carlos Garcı́a

et al.

Toxins, Journal Year: 2025, Volume and Issue: 17(3), P. 126 - 126

Published: March 7, 2025

Cyanobacterial harmful algal blooms (cyanoHABs) are a natural phenomenon produced mainly by the interaction between and anthropogenic events. CyanoHABs characterized production of cyanotoxins that can have effects on different species within food web even affect human health. Among most prevalent toxin groups worldwide microcystins (MCs), anatoxins (ATXs), cylindrospermopsins (CYNs) nodularins (NODs), which as toxins with hepatotoxic, neurotoxic, cytotoxic effects. This review summarizes analyzes research influence cyanoHABs, main toxin-producing cyanobacteria in freshwater marine bodies, highlighting their global occurrence, toxicology, bioaccumulation dynamics vectors web, cases acute chronic intoxications humans. is useful for understanding cyanoHABs’ ecosystem impact health, how implementation surveillance management framework could generate vital information stakeholders to establish health guidelines risks hazards cyanoHABs

Language: Английский

Citations

0