Improving Biosensors by the Use of Different Nanomaterials: Case Study with Microcystins as Target Analytes DOI Creative Commons

Hanbin Park,

Gahyeon Kim,

Yoseph Seo

et al.

Biosensors, Journal Year: 2021, Volume and Issue: 11(12), P. 525 - 525

Published: Dec. 20, 2021

The eutrophication of lakes and rivers without adequate rainfall leads to excessive growth cyanobacterial harmful algal blooms (CyanoHABs) that produce toxicants, green tides, unpleasant odors. rapid CyanoHABs owing global warming, climate change, the development rainforests dams considering environmental concern towards is a serious issue. Humans livestock consuming toxicant-contaminated water originated from suffer severe health problems. Among various toxicants produced by CyanoHABs, microcystins (MCs) are most harmful. Excess accumulation MC within living organisms can result in liver failure hepatocirrhosis, eventually leading death. Therefore, it essential precisely detect MCs samples. To date, liquid chromatography-mass spectrometry (LC-MS) enzyme-linked immunosorbent assay (ELISA) have been standard methods for detection provide precise results with high reliability. However, these require heavy instruments complicated operation steps could hamper portability field-readiness system. order this goal be achieved, biosensor has attracted powerful alternative detection. Thus far, several types proposed freshwater sample. introduction material useful option improve performance construct new biosensors. Introducing nanomaterials interface provides phenomena or enhances sensitivity. In recent times, different nanomaterials, such as metallic, carbon-based, transition metal dichalcogenide-based developed used fabricate biosensors This study reviews advancements nanomaterial-based

Language: Английский

Impacts of algal blooms and microcystins in fish on small-scale fishers in Winam Gulf, Lake Victoria: implications for health and livelihood DOI Creative Commons
Amber Roegner, Jessica R. Corman, Lewis Sitoki

et al.

Ecology and Society, Journal Year: 2023, Volume and Issue: 28(1)

Published: Jan. 1, 2023

Lake Victoria, bordered by Kenya, Tanzania, and Uganda, provides one of the largest freshwater fisheries in world supports millions small-scale fishing communities. Historical environmental change, including population growth, nutrient loading, introduced invasive species, rising temperatures, has resulted eutrophication persistent cyanobacterial harmful algae blooms (cyanoHABs) over recent decades, particularly shallower gulfs, bays, inlets. CyanoHABs impact food web dynamics compromise water security for nearshore fisher populations. In this study, we examine social-ecological on health these eutrophic regions, Winam Gulf Victoria. persist months produce microcystins hepatotoxins at levels unsafe human health. We assessed potential risk contribution microcystin exposure through fish consumption, addition to source, conducted 400 household surveys. Average concentrations exceeded World Health Organization (WHO) guideline drinking consistently during long dry season, cell counts surpassed WHO standards recreational 84% samples. Hazard quotients consumed young children were 5 10 times higher than permissible levels. addition, fishers chronicled profound ecosystem changes with direct livelihood, fisheries, quality 77.4% reporting a decline profit or catch, 83.1% adverse impacts cyanoHABs lake, 98.2% indicators declining lake overall. Through application lens public model, identified spheres influence that modify how experience HABs related stressors risks provide starting point which identify sustainable strategies improve livelihood

Language: Английский

Citations

12
LC-MS/MS Analysis of Cyanotoxins in Bivalve Mollusks—Method Development, Validation and First Evidence of Occurrence of Nodularin in Mussels (Mytilus edulis) and Oysters (Magallana gigas) from the West Coast of Sweden DOI Creative Commons
Julio César España Amórtegui, Heidi Pekar, Mark Dennis Chico Retrato

et al.

Toxins, Journal Year: 2023, Volume and Issue: 15(5), P. 329 - 329

Published: May 11, 2023

In this paper, an LC-MS/MS method for the simultaneous identification and quantification of cyanotoxins with hydrophilic lipophilic properties in edible bivalves is presented. The includes 17 comprising 13 microcystins (MCs), nodularin (NOD), anatoxin-a (ATX-a), homoanatoxin (h-ATX) cylindrospermopsin (CYN). A benefit to presented possibility MS detection MC-LR-[Dha7] MC-LR-[Asp3] as separately identified MS-resolved MRM signals, two congeners which were earlier detected together. performance was evaluated by in-house validation using spiked mussel samples range 3.12-200 µg/kg. found be linear over full calibration all included except CYN a quadratic regression used. showed limitations MC-LF (R2 = 0.94), MC-LA ≤ 0.98) MC-LW 0.98). recoveries ATX-a, h-ATX, CYN, NOD, lower than desired (<70%), but stable. Despite given limitations, results that specific robust investigated parameters. demonstrate suitability applied reliable monitoring tool group cyanotoxins, well highlight compromises need if multi-toxin methods are used analysis broader chemical properties. Furthermore, analyze mussels (Mytilus edulis) oysters (Magallana gigas) collected 2020-2022 summers along coast Bohuslän (Sweden). complementary qualitative presence phytoplankton from marine waters around southern Sweden performed method. Nodularin quantified bivalve 7-397 Toxins produced cyanobacteria not European Union regulatory bivalves; thus, study can useful providing basis future work including within frame increase seafood safety.

Language: Английский

Citations

11
Irrigation-dependent accumulation of microcystin in different crops under mid-scale greenhouse conditions DOI Creative Commons
Wannes Hugo R. Van Hassel,

Elise Tardy,

B. G. Cottyn

et al.

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101753 - 101753

Published: Feb. 1, 2025

Language: Английский

Citations

0
Uptake of the cyanobacterial toxin microcystin by crop plants irrigated with contaminated wastewater: a review DOI Creative Commons
Stephen P. Faulkner, Crystal Sweetman,

John Hutson

et al.

Reviews in Environmental Science and Bio/Technology, Journal Year: 2025, Volume and Issue: unknown

Published: March 2, 2025

Language: Английский

Citations

0
Health and Environmental Impacts of Cyanobacteria and Cyanotoxins from Freshwater to Seawater DOI Creative Commons

Tamara Villalobos,

Benjamín A. Suárez‐Isla,

Carlos Garcı́a

et al.

Toxins, Journal Year: 2025, Volume and Issue: 17(3), P. 126 - 126

Published: March 7, 2025

Cyanobacterial harmful algal blooms (cyanoHABs) are a natural phenomenon produced mainly by the interaction between and anthropogenic events. CyanoHABs characterized production of cyanotoxins that can have effects on different species within food web even affect human health. Among most prevalent toxin groups worldwide microcystins (MCs), anatoxins (ATXs), cylindrospermopsins (CYNs) nodularins (NODs), which as toxins with hepatotoxic, neurotoxic, cytotoxic effects. This review summarizes analyzes research influence cyanoHABs, main toxin-producing cyanobacteria in freshwater marine bodies, highlighting their global occurrence, toxicology, bioaccumulation dynamics vectors web, cases acute chronic intoxications humans. is useful for understanding cyanoHABs’ ecosystem impact health, how implementation surveillance management framework could generate vital information stakeholders to establish health guidelines risks hazards cyanoHABs

Language: Английский

Citations

0
Experimental accumulation and depuration kinetics and natural occurrence of microcystin-LR in basil (Ocimum basilicum L.) DOI
Wannes Hugo R. Van Hassel, Mohamed F. Abdallah,

Maria Gracia Guzman Velasquez

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 347, P. 123715 - 123715

Published: March 8, 2024

Language: Английский

Citations

3
Cyanotoxins Increase Cytotoxicity and Promote Nonalcoholic Fatty Liver Disease Progression by Enhancing Cell Steatosis DOI Creative Commons
Suryakant K. Niture,

Sashi Gadi,

Qi Qi

et al.

Toxins, Journal Year: 2023, Volume and Issue: 15(7), P. 411 - 411

Published: June 25, 2023

Freshwater prokaryotic cyanobacteria within harmful algal blooms produce cyanotoxins which are considered major pollutants in the aquatic system. Direct exposure to through inhalation, skin contact, or ingestion of contaminated drinking water can target liver and may cause hepatotoxicity. In current study, we investigated effect low concentrations on cytotoxicity, inflammation, modulation unfolded protein response (UPR), steatosis, fibrosis signaling human hepatocytes cell models. Exposure microcystin-LR (MC-LR), microcystin-RR (MC-RR), nodularin (NOD), cylindrospermopsin (CYN) bipotent progenitor line HepaRG hepatocellular carcinoma (HCC) lines HepG2 SK-Hep1 resulted increased toxicity. MC-LR, NOD, CYN differentially regulated inflammatory signaling, activated UPR lipogenic gene expression, induced cellular steatosis fibrotic HCC cells. also AKT/mTOR inhibited autophagy. Chronic upregulated expression biomarkers. Moreover, RNA sequencing (RNA seq) data suggested that hepatocytes, HepaRG, cells MC-LR modulated levels several genes regulate non-alcoholic fatty disease (NAFLD). Our suggest hepatotoxicity promote NAFLD progression.

Language: Английский

Citations

9
Natural toxins and One Health: a review DOI Creative Commons
Azubuike Raphael Nwaji, Onikisateinba Arieri, Annabel Sharon Anyang

et al.

Science in One Health, Journal Year: 2022, Volume and Issue: 1, P. 100013 - 100013

Published: Nov. 1, 2022

The One Health concept considers the interconnectivity, interactions and interdependence of humans, animals environment. Humans, other organisms are constantly exposed to a wide range natural toxins present in Thus, there is growing concern about potential detrimental effects that could pose achieve Health. Interestingly, alkaloids, steroids bioactive peptides obtained from be used for development therapeutic agents. Our literature search focused on following keywords; toxins, Health, microbial mycotoxins, phytotoxins, phycotoxins, insect toxin effects. Google Scholar, Science Direct, PubMed Web were engines obtain primary databases. We chose relevant full-text articles review papers published English language only. research was done between July 2022 January 2023. Natural poisonous substances comprising compounds produced by microorganisms, invertebrates, plants animals. These possess diverse structures differ biological function toxicity, posing risks human animal health through contamination environment, causing disease or death certain cases. Findings reviewed revealed humans gained more attention than impact environment lower organisms, irrespective significant roles play maintain ecosystem balance. Also, systematic approaches control utilization beneficial purposes inadequate many regions. Remarkably, have findings suggest global, comprehensive coordinated efforts required improved management an interdisciplinary, approach. Adopting approach critical addressing

Language: Английский

Citations

13
Chlorination of microcystin-LR in natural water: Kinetics, transformation products, and genotoxicity DOI

Jinlu Feng,

Xi Li, Habasi Patrick Manzi

et al.

Journal of Environmental Management, Journal Year: 2023, Volume and Issue: 338, P. 117774 - 117774

Published: March 28, 2023

Language: Английский

Citations

7
Mutations at Two Key Sites in PP2A Safeguard Caenorhabditis elegans Neurons from Microcystin-LR Toxicity DOI Creative Commons
Chunhua Zhan, Jianke Gong

Toxins, Journal Year: 2024, Volume and Issue: 16(3), P. 145 - 145

Published: March 13, 2024

Microcystin-LR (MC-LR) is a secondary metabolite produced by cyanobacteria, globally renowned for its potent hepatotoxicity. However, an increasing body of research suggests that it also exhibits pronounced neurotoxicity. PP2A fundamental intracellular phosphatase plays pivotal role in cell development and survival. Although extensive has focused on the binding MC-LR to C subunit PP2A, few studies have explored key amino acid sites can prevent PP2A-C. Due advantages Caenorhabditis elegans (C. elegans), such as ease genetic editing short lifespan, we exposed nematodes manner simulated natural exposure conditions based concentrations water bodies (immersion exposure). Our findings demonstrate exerts comprehensive toxicity nematodes, including reducing impairing reproductive capabilities, diminishing sensory functions. Notably, first time, observed neurotoxic effects persist up F3 generation, highlighting significant threat poses biological populations environments. Furthermore, identified two (L252 C278) PP2A-C through mutations prevented without affecting activity. This discovery was robustly validated behavioral neuronal calcium imaging using nematodes. In conclusion, crucial could from PP2A-C, which holds great significance future detoxification drugs.

Language: Английский

Citations

2