Fluorescent solid-state strips based on SiO2 shell-stabilized perovskite nanocrystals applying for magnetic aptasensing detection of aflatoxin B1 toxin in food

Food Chemistry, Journal Year: 2024, Volume and Issue: 449, P. 139316 - 139316

Published: April 10, 2024

Language: Английский

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Effective Physical Methods for Aflatoxin B1 Removal in Food: A Comprehensive Review

Food Control, Journal Year: 2025, Volume and Issue: unknown, P. 111215 - 111215

Published: Feb. 1, 2025

Language: Английский

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Target-Induced Electrochemical Sensor Based on Foldable Aptamer and MoS₂@MWCNTs–PEI for Enhanced Detection of AFB1 in Peanuts

Langmuir, Journal Year: 2023, Volume and Issue: 39(46), P. 16422 - 16431

Published: Nov. 7, 2023

Herein, a sensitive and selective electrochemical sensor based on aptamer folding was constructed to detect aflatoxin B1 (AFB1) in peanuts. Specifically, polyethylenimine-functionalized multiwalled carbon nanotubes modified with molybdenum disulfide (MoS2@MWCNTs–PEI) were used as the electrode matrix enable large specific surface area, which characterized by Randles–Sevcik equation. Additionally, AuNPs immobilize via Au–S covalent bond provide favorable microenvironment for signal enhancement. Methylene blue (MB) at proximal 3′ termini of capture probe, while transduction obtained through changes conformation position MB induced binding between AFB1 probe. Changes spatial could be recorded methods more readily. This aptasensor demonstrated remarkable sensitivity an extensive detection range (1 pg/mL 100 ng/mL) lower limit (1.0 × 10–3 ng/mL). Moreover, using aptasensor, identified successfully peanut samples, recoveries ranging from 95.83 107.53%, illustrating its potential use determining food.

Language: Английский

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Detection of Maize Mold Based on a Nanocomposite Colorimetric Sensor Array under Different Substrates

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(19), P. 11164 - 11173

Published: April 2, 2024

This study developed a novel nanocomposite colorimetric sensor array (CSA) to distinguish between fresh and moldy maize. First, the headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC/MS) method was used analyze volatile organic compounds (VOCs) in maize samples. Then, principal component analysis orthogonal partial least-squares discriminant (OPLS-DA) were identify 2-methylbutyric acid undecane as key VOCs associated with Furthermore, sensitive dyes modified different nanoparticles employed enhance dye properties CSA of VOCs. focused on synthesizing four types nanoparticles: polystyrene acrylic (PSA), porous silica nanospheres (PSNs), zeolitic imidazolate framework-8 (ZIF-8), ZIF-8 after etching. Additionally, three substrates, qualitative filter paper, polyvinylidene fluoride film, thin-layer chromatography gel, comparatively fabricate combining linear (LDA) K-nearest neighbor (KNN) models for real sample detection. All samples correctly identified prepared characterize CSA. Through initial testing nanoenhancement chosen dyes, confirmed. The accuracy rates LDA KNN this reached 100%. work shows great potential grain quality control using methods.

Language: Английский

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Aflatoxin B1-induced hepatotoxicity through mitochondrial dysfunction, oxidative stress, and inflammation as central pathological mechanisms: A review of experimental evidence

Toxicology, Journal Year: 2024, Volume and Issue: 509, P. 153983 - 153983

Published: Nov. 2, 2024

Aflatoxin B

Language: Английский

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In situ synthesized eRAFT polymers for highly sensitive electrochemical determination of AFB1 in foods and herbs

Food Chemistry, Journal Year: 2023, Volume and Issue: 421, P. 136176 - 136176

Published: April 18, 2023

Language: Английский

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Technologies to decontaminate aflatoxins in foods: a review

International Journal of Food Science & Technology, Journal Year: 2024, Volume and Issue: 59(10), P. 6783 - 6796

Published: Aug. 16, 2024

Summary Aflatoxins are toxic secondary metabolites produced by Aspergillus spp., found in staple food commodities. carcinogenic, teratogenic, and mutagenic pose a serious threat to the health of humans. The identification quantification aflatoxins foods is major challenge guarantee safety. Therefore, developing feasible, sensitive, robust methods for decontamination paramount, with short processing time negligible impact on quality. This review evaluates recent novel technologies physical (microwave heating, Gama electron beam irradiation, pulse light ultraviolet), chemical (ozone, natural plant extracts, organic acids), biological (atoxigenic strains, Trichoderma bacteria yeast). study highlights cutting‐edge smart packaging artificial intelligence (AI). To achieve more efficiency adaptability different matrices decontamination, suggests integrating multiple strategies. also recommends Partnership Delivery (P4D) share responsibility increase chance success control foods.

Language: Английский

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Pesticide and Mycotoxin Residues in Astragalus: Transfer Patterns, Processing Factors and Risk Assessment During Astragalus Processing

Published: Jan. 1, 2025

Language: Английский

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Effects of AFB1 and Aspergillus flavus Spores on Root Rhizospheric Fungal Population, Seedling Emergence, Plant Growth, and Yield

Agronomy, Journal Year: 2025, Volume and Issue: 15(3), P. 523 - 523

Published: Feb. 21, 2025

Maize is susceptible to fungal infections that can lead aflatoxin B1 (AFB1) contamination, and this could have dire consequences on plant growth yield. The objectives of study were determine the effects AFB1 Aspergillus flavus fungus root rhizospheric population, maize seedling emergence, growth, A randomized complete block design (RCBD) experiment was conducted in two sites 5 (AFB1/A. spores’ treatments) × 3 replicates 12 pots per experimental unit. This showed there a significant (p < 0.05) increase colony forming unit (CFU) with A. soil effect reducing pH. It observed all exposed spores, linear decrease emergence percentage speed spores soil, whereby 320 ppb/kg also reported lowest (51.4%), rate index (5.0), (2.06). exerted depressive height number leaves, thus grain yield plant. had non-significant when compared AFB1.

Language: Английский

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Aflatoxin B1 (AFB1) biodegradation by a lignolytic phenoloxidase of Trametes hirsuta

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 21, 2025

Abstract Aflatoxin B 1 (AFB ) is a highly potent mycotoxin that poses serious threat to human and animal health. This study investigated the biodegradation of AFB by supernatant submerged cultured Trametes hirsuta , with focus on identifying characterizing responsible enzyme(s). The extracellular enzymes white-rot mushroom were extracted from pre-separated using anion exchange fast protein liquid chromatography (FPLC). To pinpoint specific enzyme, eluted fractions exhibiting highest degradation activity subjected detailed biochemical proteomic analyses. A second purification step, ultrafiltration, yielded an electrophoretically pure enzyme. Sequencing tryptic peptides nano-LC system coupled qQTOF mass spectrometer identified enzyme as lignolytic phenoloxidase. exhibited molecular 55.6 kDa achieved impressive rate 77.9% under optimized experimental conditions. first fungal phenoloxidase capable aflatoxin without requiring hydrogen peroxide cofactor, highlighting its unique catalytic mechanism. It may be used in remediation strategies, such treating surfaces contaminated fruits, vegetables, nuts.

Language: Английский

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