Toxins, Journal Year: 2023, Volume and Issue: 15(11), P. 639 - 639

Published: Nov. 1, 2023

Of the wide variety of toxic compounds produced by cyanobacteria, neurotoxic amino acid β-N-methylamino-l-alanine (BMAA) has attracted attention as a result its association with chronic human neurodegenerative diseases such ALS and Alzheimer’s. Consequently, specific detection methods are required to assess presence BMAA isomers in environmental clinical materials, including cyanobacteria mollusks. Although separation β-amino-N-methylalanine (BAMA), N-(2-aminoethyl)glycine (AEG) 2,4-diaminobutyric (DAB) from been demonstrated during routine analysis, further compounding factor is potential enantiomers for some these isomers. Current analytical mostly do not discriminate between enantiomers, chiral configuration still largely unexplored. To understand occurrence D-BMAA UPLC-MS/MS method was developed separate determine enantiomeric endogenous free marine Lyngbya mat two mussel reference materials. After extraction, purification derivatization N-(4-nitrophenoxycarbonyl)-l-phenylalanine 2-methoxyethyl ester ((S)-NIFE), both L- were identified acids cyanobacterial whereas only L-BMAA tissues. The finding biological materials raises questions concerning source role neurological disease.

Language: Английский