A Direct Analysis of β-N-methylamino-l-alanine Enantiomers and Isomers and Its Application to Cyanobacteria and Marine Mollusks DOI Creative Commons
James S. Metcalf, Sandra Anne Banack, Peter B. Wyatt

et al.

Toxins, Journal Year: 2023, Volume and Issue: 15(11), P. 639 - 639

Published: Nov. 1, 2023

Of the wide variety of toxic compounds produced by cyanobacteria, neurotoxic amino acid β-N-methylamino-l-alanine (BMAA) has attracted attention as a result its association with chronic human neurodegenerative diseases such ALS and Alzheimer’s. Consequently, specific detection methods are required to assess presence BMAA isomers in environmental clinical materials, including cyanobacteria mollusks. Although separation β-amino-N-methylalanine (BAMA), N-(2-aminoethyl)glycine (AEG) 2,4-diaminobutyric (DAB) from been demonstrated during routine analysis, further compounding factor is potential enantiomers for some these isomers. Current analytical mostly do not discriminate between enantiomers, chiral configuration still largely unexplored. To understand occurrence D-BMAA UPLC-MS/MS method was developed separate determine enantiomeric endogenous free marine Lyngbya mat two mussel reference materials. After extraction, purification derivatization N-(4-nitrophenoxycarbonyl)-l-phenylalanine 2-methoxyethyl ester ((S)-NIFE), both L- were identified acids cyanobacterial whereas only L-BMAA tissues. The finding biological materials raises questions concerning source role neurological disease.

Language: Английский

Stalling the Course of Neurodegenerative Diseases: Could Cyanobacteria Constitute a New Approach toward Therapy? DOI Creative Commons
Vitória Ramos, Mariana Reis, Leonor Vasconcelos Ferreira

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(10), P. 1444 - 1444

Published: Sept. 25, 2023

Neurodegenerative diseases (NDs) are characterized by progressive and irreversible neuronal loss, accompanied a range of pathological pathways, including aberrant protein aggregation, altered energy metabolism, excitotoxicity, inflammation, oxidative stress. Some the most common NDs include Alzheimer’s Disease (AD), Parkinson’s (PD), Multiple Sclerosis (MS), Amyotrophic Lateral (ALS), Huntington’s (HD). There currently no available cures; there only therapeutic approaches that ameliorate progression symptoms, which makes search for new drugs targets constant battle. Cyanobacteria ancient prokaryotic oxygenic phototrophs whose long evolutionary history has resulted in production plethora biomedically relevant compounds with anti-inflammatory, antioxidant, immunomodulatory, neuroprotective properties, can be valuable this field. This review summarizes major their pathophysiology, focus on anti-neurodegenerative properties cyanobacterial main effects.

Language: Английский

Citations

5
Cyanotoxin Occurrence and Diversity in 98 Cyanobacterial Blooms From Swedish Lakes and the Baltic Sea DOI Open Access

Caroline Dirks,

Paolo Cappelli,

Maria Blomqvist

et al.

Published: April 4, 2024

The Drinking Water Directive (EU) 2020/2184 includes the parameter microcystin-LR, a cyanotoxin, which drinking water producers need to analyze if source has potential for cyanobacterial blooms. In light of increasing occurrences blooms worldwide and given that more than 50 percent in Sweden is produced from surface water, both fresh brackish, improved knowledge about cyanotoxin occurrence diversity increased. this study total 98 were sampled 2016-2017 identified regarding their toxin production taxonomical compositions. samples freshwater lakes throughout including brackish 8 east coast locations along Baltic Sea analyzed content with LC-MS/MS taxonomic composition 16S rRNA amplicon sequencing. Both extracellular was analyzed. Microcystin´s prevalence highest presence 82% blooms, as free toxins 39% Saxitoxins found 36% congener decarbamoylsaxitoxin (dcSTX) detected first time Swedish waters at four sampling sites. Anatoxins most rarely detected, followed by cylindrospermopsin, 6% 10% samples, respectively. As expected, nodularin collected only. operational unit (OTU) abundance could be annotated Aphanizomenon NIES-81 second profuse taxon Microcystis PCC-7914. addition, two correlations found, one between saxitoxins another PCC-7914 microcystins. This value management scientists involved recognizing controlling toxic cyanobacteria

Language: Английский

Citations

1
Cyanotoxin Occurrence and Diversity in 98 Cyanobacterial Blooms from Swedish Lakes and the Baltic Sea DOI Creative Commons

Caroline Dirks,

Paolo Cappelli,

Maria Blomqvist

et al.

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(5), P. 199 - 199

Published: April 27, 2024

The Drinking Water Directive (EU) 2020/2184 includes the parameter microcystin LR, a cyanotoxin, which drinking water producers need to analyze if source has potential for cyanobacterial blooms. In light of increasing occurrences blooms worldwide and given that more than 50 percent in Sweden is produced from surface water, both fresh brackish, improved knowledge about cyanotoxin occurrence diversity increased. this study, total 98 were sampled 2016-2017 identified based on their toxin production taxonomical compositions. samples freshwater lakes throughout including brackish eight east coast locations along Baltic Sea analyzed content with LC-MS/MS taxonomic composition 16S rRNA amplicon sequencing. Both extracellular analyzed. Microcystin's prevalence was highest presence 82% blooms, as free 39% Saxitoxins found 36% congener decarbamoylsaxitoxin (dcSTX) detected first time Swedish waters at four sampling sites. Anatoxins most rarely detected, followed by cylindrospermopsin, 6% 10% samples, respectively. As expected, nodularin collected only. operational units (OTUs) abundance could be annotated Aphanizomenon NIES-81 second profuse taxon Microcystis PCC 7914. addition, two correlations found, one between saxitoxins another 7914 microcystins. This study value management scientists involved recognizing controlling toxic cyanobacteria

Language: Английский

Citations

1
From colonial clusters to colonial sheaths: Imaging flow cytometry analysis of Microcystis morphospecies dynamics in mesocosm and links to CyanoHABs management DOI Creative Commons

Adina Zhumakhanova,

Yersultan Mirasbekov,

Ayagoz Meirkhanova

et al.

Ecological Indicators, Journal Year: 2024, Volume and Issue: 163, P. 112100 - 112100

Published: May 8, 2024

The alarming increase in the frequency of blooms Microcystis freshwater lakes and reservoirs occurs worldwide, with major implications for their ecosystem functioning water quality. dominance is tightly related to colonial formation by Microcystis. However, studies development morphospecies are rare. This research applied FlowCAM-based imaging flow cytometry analyze mesocosms mimicking eutrophic shallow effect temperature changes. A significant positive association was found between M. ichtyoblabe, aeruginosa, smithii colonies, particularly high-temperature tanks, suggesting that these belong one ecocluster, which supports hypothesis central transition pathways small clusters cells represented an important stage sequence bloom were associated forms. correlation analysis showed higher pH positively correlated abundance M.wesenbergii independently sheaths' abundances increased following a maximum abundance, reaching numbers (thousands), majority sheaths contained at least some cells. We hypothesize may be crucial spp. dispersal represent obligatory colonies development. protect against environmental stress factors, improve cell survival low nutrient levels, participate spreading. Our findings can applicable early CyanoHAB detection management dispersal.

Language: Английский

Citations

1
Insight in limited research on environmental factors and health implications of toxic cyanobacteria bloom in African freshwater bodies DOI Creative Commons

Nadia Elidrissi El Yallouli,

Majida Lahrouni,

Richard Mugani

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 21(1)

Published: Nov. 20, 2024

The rapid rise of cyanotoxins production poses a serious threat to global freshwater ecosystems and has become an important issue public health. In Africa, continent facing environmental problems, including poisoning by cyanotoxins, knowledge the presence distribution these toxins remains limited. primary objective our review is assess occurrence particularly microcystins (MCs), in African ecosystems. We aim analyze limited but existing literature on this subject, focusing specifically studies published between 2001 2023. Recognizing scarcity research topic we strive elucidate emerging patterns toxin variants their correlation with variables, such as water temperature nutrient concentrations. Our investigation reveals MCs predominant cyanobacterial secondary metabolites detected across majority environments where have been documented. Furthermore, concentrations exceeded World Health Organization's (WHO) provisional guideline for lifetime human exposure through drinking water, which set at 1 µg L−1, indicated data Table 1. Highly significant positive relationships were found toxin-producing cyanobacteria factors, particular Given associated risks recommend frequent monitoring quality, development guidelines based international best practice, toxicity tests, epidemiological investigations understand health impacts cyanotoxin exposure. These measures are essential both protect well-being humans ensure sustainability aquatic

Language: Английский

Citations

1
Temporal Dynamics of Cyanobacterial Bloom Community Composition and Toxin Production from Urban Lakes DOI Creative Commons

Julie A. Maurer,

Andrew M. Kim,

Nana Oblie

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 10, 2024

ABSTRACT With a long evolutionary history and need to adapt changing environment, cyanobacteria in freshwater systems use specialized metabolites for communication, defense, physiological processes. However, the role that these play differentiating species, maintaining microbial communities, generating niche persistence expansion is poorly understood. Furthermore, many cyanobacterial toxins present significant human health concerns due their liver toxicity potential impact drinking water. Gaps knowledge exist with respect changes species diversity toxin production during bloom (cyanoHAB) event; addressing gaps will improve understanding of impacts public ecological health. In current project, we utilized multi-omics strategy (DNA metabarcoding metabolomics) determine community composition, profile, metabolite pool at three lakes Providence, RI summer-fall cyanoHABs. Species decreased all study sites over course event, reached maximum midpoint event. Additionally, LC-MS/MS-based molecular networking identified new congeners. This work provokes intriguing questions allelopathy by organisms presence emerging toxic compounds can SYNOPSIS reports on succession dynamics events. Results show relationships temporal are relevant

Language: Английский

Citations

0
Regional Differences in Microbial Infiltration of Brain Tissue from Alzheimer’s Disease Patients and Control Individuals DOI Creative Commons

T. Bucky Jones,

Ping Chu,

Brooke Wilkey

et al.

Brain Sciences, Journal Year: 2024, Volume and Issue: 14(7), P. 677 - 677

Published: July 3, 2024

Alzheimer's disease (AD) is characterized by cognitive decline and neuropathology including amyloid beta (Aβ) plaques neurofibrillary tangles (tau). Factors initiating or driving these pathologies remain unclear, though microbes have been increasingly implicated. Our data others' findings indicate that may be common constituents of the brain. It notable Aβ tau antimicrobial properties, suggesting a response to in We used 16S rRNA sequencing compare major bacterial phyla post-mortem tissues from individuals exhibiting range status two brain regions variably affected AD. strong regional differences exist, driven part varied presence Proteobacteria Firmicutes. confirmed our using ELISA lipopolysaccharide (LPS) lipoteichoic acid same tissue. identified potential association between composition but not status. Declining cognition increasing pathology correlated closely with serum LPS, levels although LPS showed negative correlation cerebral angiopathy. Collectively, suggest region-specific heterogeneity microbial populations tissue potentially associated neurodegenerative pathology.

Language: Английский

Citations

0
Temporal Dynamics of Cyanobacterial Bloom Community Composition and Toxin Production from Urban Lakes DOI

Julie A. Maurer,

Runjie Xia,

Andrew M. Kim

et al.

ACS ES&T Water, Journal Year: 2024, Volume and Issue: 4(8), P. 3423 - 3432

Published: July 18, 2024

With a long evolutionary history and need to adapt changing environment, cyanobacteria in freshwater systems use specialized metabolites for communication, defense, physiological processes. Furthermore, many cyanobacterial toxins present significant human health concerns due their liver toxicity potential impact drinking water. Gaps knowledge exist with respect changes species diversity toxin production during bloom (cyanoHAB) event; addressing these gaps will improve understanding of impacts public ecological health. In the current report we detail community composition dynamics late period. Species decreased at all study sites over course event, reached maximum midpoint event. We also isolated three new microcystins from Microcystis dominated (1–3), two which contained unusual doubly homologated tyrosine residues (1 2). This work provokes intriguing questions allelopathy by organisms presence emerging toxic compounds that can

Language: Английский

Citations

0
Impact of Algicidal Fungus Aspergillus Welwitschiae Gf6 on Harmful Bloom-Forming Cyanobacterium Microcystis Aeruginosa: Growth and Physiological Responses DOI

И. Л. Кузикова,

T. B. Zaytseva,

Ekaterina Chernova

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
CRISPR driven Cyanobacterial Metabolic Engineering and its role in metabolite production DOI Open Access
S. Chakraborty, Rameswar Mukhopadhyay, Rohan Dutta

et al.

Journal of Experimental Biology and Agricultural Sciences, Journal Year: 2024, Volume and Issue: 12(3), P. 446 - 456

Published: July 15, 2024

Recently, the advancement in sustainable methods for fabricating novel metabolites is one of prime challenges metabolic engineering. The current increase fuel prices and its limited supply made scientific community more concerned about finding an alternate source generation. Scientists are now interested biofuel because low cost ease production. An intriguing area research engineering using imaginative manipulation microbes to manufacture chemicals or molecules commercial importance. One such bacterium whose potential rapidly attracting attention fraternity Cyanobacteria, which either single-celled multi-cellular filamentous photosynthetic organisms that can also fix CO2. generation has been transformed by use CRISPR (clustered regularly interspaced short palindromic repeats) technology cyanobacteria, allows precise genetic alterations improve their processes. effectively modify cyanobacterial genome lipid accumulation, maximize efficiency, enhance stress tolerance. Cyanobacteria have gained as a production due several advantageous characteristics like capacity, manipulation, lack dependency on fertile land, high biomass yield, versatile etc. our present manuscript aims catalogue. play pivotal role developing environmentally friendly energy solutions converting CO2 into renewable sources, serving flexible platform producing different types biofuels reducing greenhouse gas emissions.

Language: Английский

Citations

0