Metagenomic insights into cyanotoxin dynamics in a Mexican subtropical lake DOI Creative Commons
Nicolas Tromas, Dana F. Simon, Nathalie Fortin

et al.

Chemosphere, Journal Year: 2025, Volume and Issue: 376, P. 144285 - 144285

Published: March 9, 2025

Valle de Bravo is a vital water supply for part of the metropolitan area Mexico megacity, providing 30% City's demand. This body has experienced an acceleration in its trophic status, going from oligotrophic to eutrophic just few years. temperate lake (at tropical latitude) persistent bloom dominated by variety co-occurring cyanobacteria, many which have toxigenic potential based on microscopic identification, that makes it difficult or even impractical identify cyanotoxin producers. To unravel this complexity and directly genera, we showed integrating classical approaches with metagenomic required. We first characterized, genes metagenomes assembled genomes, Cyanobacteria. found Microcystis was most dominant cyanobacterial genus sole carrier mcy operon, making only microcystin producer. then quantified twenty-one different cyanopeptides, including twelve congeners using high-performance liquid chromatography-high-resolution. Nine microcystins (MCs) emerging anabaenopeptin-A -B were at varying concentrations throughout year, MC-LA being common abundant. Our findings, constrained our sampling strategy, indicate conventional biomarkers (e.g., toxin genes) not consistently reliable indicators freshwater system. In study, followed dynamics community associated cyanopeptides unprecedented resolution. results implications better management toxic blooms system, supplies drinking more than 7 million people megalopolis México.

Language: Английский

Metagenomic insights into cyanotoxin dynamics in a Mexican subtropical lake DOI Creative Commons
Nicolas Tromas, Dana F. Simon, Nathalie Fortin

et al.

Chemosphere, Journal Year: 2025, Volume and Issue: 376, P. 144285 - 144285

Published: March 9, 2025

Valle de Bravo is a vital water supply for part of the metropolitan area Mexico megacity, providing 30% City's demand. This body has experienced an acceleration in its trophic status, going from oligotrophic to eutrophic just few years. temperate lake (at tropical latitude) persistent bloom dominated by variety co-occurring cyanobacteria, many which have toxigenic potential based on microscopic identification, that makes it difficult or even impractical identify cyanotoxin producers. To unravel this complexity and directly genera, we showed integrating classical approaches with metagenomic required. We first characterized, genes metagenomes assembled genomes, Cyanobacteria. found Microcystis was most dominant cyanobacterial genus sole carrier mcy operon, making only microcystin producer. then quantified twenty-one different cyanopeptides, including twelve congeners using high-performance liquid chromatography-high-resolution. Nine microcystins (MCs) emerging anabaenopeptin-A -B were at varying concentrations throughout year, MC-LA being common abundant. Our findings, constrained our sampling strategy, indicate conventional biomarkers (e.g., toxin genes) not consistently reliable indicators freshwater system. In study, followed dynamics community associated cyanopeptides unprecedented resolution. results implications better management toxic blooms system, supplies drinking more than 7 million people megalopolis México.

Language: Английский

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