Integration of Fatty Acid-Targeted Metabolome and Transcriptomics Reveals the Mechanism of Chronic Environmental Microcystin-LR-Induced Hepatic Steatosis

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Microcystin-LR (MC-LR) is a toxin that causes hepatic steatosis. Our previous study found exposure to 60 μg/L MC-LR for 9 months resulted in liver lipid accumulation, but the underlying mechanisms remain elusive. Herein, first time, fatty acid-targeted metabolome and RNA-seq were combined probe effect mechanism of chronic (12-month) treatment on mice metabolism at environmental-related levels (1, 60, 120 μg/L). It was dose-dependently raised serum levels. The total cholesterol (TC) significantly increased following with 1 (equivalent 0.004 μ/L human). Treatment elevated TC triglyceride (TG) both liver. Serum analysis demonstrated 1, caused significant alterations acid profile. Chronic polyunsaturated acids (PUFAs), including conjugated linoleic eicosapentaenoic acid, which positively correlated or TG led decrease accumulation saturated acids, citramalic pentadecanoic docosanoic negatively These findings suggested mild disruption, while pronounced steatosis mice. Transcriptome revealed environmental regulated expression genes involved phosphatidylinositol 3-kinase (PI3K) complex metabolism. Western blotting RT-qPCR confirmed activated PI3K/AKT/mTOR signaling pathway, downstream fads3 gene participates desaturation upregulated, degradation-related genes, acsl1, acsl4, ehhadh inhibited, transport-related slc27a4 apol7a, promoted. Thus, boosts work indicated limit concentration human drinking water safety needs be discussed. provides evidence profile changes gains new insights into treatment-induced

Language: Английский

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Exploring the Mechanisms of Neurotoxic effects from Combined Exposure to Polystyrene and Microcystin-LR in Caenorhabditis elegans

Aquatic Toxicology, Journal Year: 2025, Volume and Issue: 284, P. 107403 - 107403

Published: May 7, 2025

Language: Английский

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Impact of Microcystin-LR exposure on biochemical responses, transcriptomic profiles, gut microbiota, and growth performance in grass carp (Ctenopharyngodon idella)

Journal of Environmental Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

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Microcystins Exposure and the Risk of Metabolic Syndrome: A Cross-Sectional Study in Central China

Toxins, Journal Year: 2024, Volume and Issue: 16(12), P. 542 - 542

Published: Dec. 14, 2024

A growing body of evidence indicates that microcystins (MCs) exposure may cause metabolic diseases. However, studies exploring the effects MCs on risk syndrome (MetS) in humans are currently lacking, and underlying mechanisms remain unclear. Here, we conducted a cross-sectional study central China to explore effect serum MetS, assessed mediation inflammation biomarker, white blood cell (WBC) level, this relationship. The relationships among WBC level MetS were using binary logistic linear regression. Mediation analysis was used possible those associations by employing R software (version 4.3.1). Compared lowest quartile MCs, highest had an increased (odds ratio [OR] = 2.10, 95% confidence interval [CI]: 1.19, 3.70), with dose–response relationship (p for trend < 0.05). WBCs mediated 11.14% association between triglyceride (TG) levels, but did not mediate MetS. This firstly reveals is independent factor manner, suggests could partially TG levels.

Language: Английский

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Ecotoxicity Assessment of Emerging Contaminants (EC) in Fish: Effects, Trends, and Challenges

˜The œhandbook of environmental chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

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