Sensitive Aflatoxin M1 Detection in Milk by ELISA: Investigation of Different Assay Configurations DOI Creative Commons

Dimitra Kourti,

Μichailia Angelopoulou,

Panagiota Petrou

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(12), P. 515 - 515

Published: Nov. 29, 2024

Aflatoxin M1 (AFM1) exposure through dairy products is associated with adverse health effects, including hepatotoxicity and carcinogenicity. Therefore, the AFM1 presence in milk strictly regulated. In this context, current work focuses on investigation of different competitive enzyme immunoassay configurations for determination high sensitivity short assay duration. Amongst tested, one based incubation anti-AFM1 specific antibody along calibrators/samples a biotinylated conjugate bovine serum albumin (BSA) microwells coated secondary provided six-fold lower detection limit than configuration involving immobilized AFM1-BSA liquid phase antibody. The achieved was 5.0 pg/mL, dynamic range up to 2.0 ng/mL. repeatable intra- inter-assay coefficients variations 3.2% 6.5%, respectively, accurate recovery values from 87.5 108%. Moreover, completed 1.5 h. excellent analytical characteristics analysis time make proposed suitable use by food industry. Furthermore, could be employed enhance immunoassays other low-molecular-weight analytes.

Language: Английский

Impact of Metabolites from Foodborne Pathogens on Cancer DOI Creative Commons
Alice Njolke Mafe, Dietrich Büsselberg

Foods, Journal Year: 2024, Volume and Issue: 13(23), P. 3886 - 3886

Published: Dec. 1, 2024

Foodborne pathogens are microorganisms that cause illness through contamination, presenting significant risks to public health and food safety. This review explores the metabolites produced by these pathogens, including toxins secondary metabolites, their implications for human health, particularly concerning cancer risk. We examine various such as Salmonella sp., Campylobacter Escherichia coli, Listeria monocytogenes, detailing specific of concern carcinogenic mechanisms. study discusses analytical techniques detecting chromatography, spectrometry, immunoassays, along with challenges associated detection. covers effective control strategies, processing techniques, sanitation practices, regulatory measures, emerging technologies in pathogen control. manuscript considers broader highlighting importance robust policies, awareness, education. identifies research gaps innovative approaches, recommending advancements detection methods, preventive policy improvements better manage foodborne metabolites.

Language: Английский

Citations

11
Recent Advances in Biosensor Technologies for Meat Production Chain DOI Creative Commons
Ivan Nastasijević, Ivana Kundačina, Stefan Jarić

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(5), P. 744 - 744

Published: Feb. 22, 2025

Biosensors are innovative and cost-effective analytical devices that integrate biological recognition elements (bioreceptors) with transducers to detect specific substances (biomolecules), providing a high sensitivity specificity for the rapid accurate point-of-care (POC) quantitative detection of selected biomolecules. In meat production chain, their application has gained attention due increasing demand enhanced food safety, quality assurance, fraud detection, regulatory compliance. can foodborne pathogens (Salmonella, Campylobacter, Shiga-toxin-producing E. coli/STEC, L. monocytogenes, etc.), spoilage bacteria indicators, contaminants (pesticides, dioxins, mycotoxins), antibiotics, antimicrobial resistance genes, hormones (growth promoters stress hormones), metabolites (acute-phase proteins as inflammation markers) at different modules along from livestock farming packaging in farm-to-fork (F2F) continuum. By real-time data biosensors enable early interventions, reducing health risks (foodborne outbreaks) associated contaminated meat/meat products or sub-standard products. Recent advancements micro- nanotechnology, microfluidics, wireless communication have further sensitivity, specificity, portability, automation biosensors, making them suitable on-site field applications. The integration blockchain Internet Things (IoT) systems allows acquired management, while artificial intelligence (AI) machine learning (ML) enables processing, analytics, input risk assessment by competent authorities. This promotes transparency traceability within fostering consumer trust industry accountability. Despite biosensors' promising potential, challenges such scalability, reliability complexity matrices, approval still main challenges. review provides broad overview most relevant aspects current state-of-the-art development, challenges, opportunities prospective applications regular use safety monitoring, clarifying perspectives.

Language: Английский

Citations

0
Advances in mycotoxin detection techniques and the crucial role of reference material in ensuring food safety. A review DOI Creative Commons

Tiisetso Colleen Maphaisa,

Oluwakamisi Festus Akinmoladun, Oluwasola Abayomi Adelusi

et al.

Food and Chemical Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 115387 - 115387

Published: March 1, 2025

Mycotoxins, toxic secondary metabolites produced by fungi, pose a significant threat to food safety and human health. The occurrence of mycotoxins in commodities necessitates accurate reliable detection methods. Advanced techniques, such as chromatographic techniques immunochemical assays, have improved sensitivity specificity. However, the lack standardized reference material, particularly less privileged countries, hinders method validation proficiency testing, ultimately affecting mycotoxin testing regulation. Moreover, these are complex they require specialized equipment, well-trained personnel, thus limiting their practical applications. This comprehensive review provides an up-to-date overview recent advancements It examines crucial role standards materials for ensuring results analysis agriculture commodities. addresses emerging challenges, knowledge gaps, future research directions material development. By synthesizing existing literature, this aims provide valuable resources researchers, policymakers, other stakeholders safety, highlighting importance integrated approaches mitigate contamination safety.

Language: Английский

Citations

0
Preparation of functionalized magnetic microporous organic network and its application in efficient extraction of aflatoxins from medicine and food homology samples DOI
Mengyao Wang,

L L Wang,

Xue Li

et al.

Journal of Food Composition and Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 107531 - 107531

Published: March 1, 2025

Language: Английский

Citations

0
Mycotoxins Diagnostic Methods from Past to Present DOI

Leila Faeli

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Mycotoxins are toxic substances produced as byproducts of various types mold. These mycotoxins mainly linked to certain genera fungi, such Aspergillus, Penicillium, Fusarium, Claviceps, and Alternaria, formed under specific conditions. encompass a range chemically diverse compounds with small molecular weight, typically less than 1000 Da. Currently, more 500 recognized harmful the health humans, animals, plants. Each type falls into particular groups distinct effects, most common well-known ones being aflatoxins (AFTs), ochratoxins (OTs), fumonisins (FUMs), zearalenone (ZEN), trichothecenes (TCTs), patulin (PAT), citrinin (CT). Adhering good agricultural industrial practices is crucial reducing risk mycotoxin contamination in food production. Efficient prompt detection utmost importance realm safety. This chapter deals conventional commercial methods for from past present.

Language: Английский

Citations

0
Enhancing mycotoxins detection through quantum dots-based optical biosensors DOI
Mehdi Dadmehr,

Farzaneh Shalileh,

Morteza Hosseini

et al.

Nanotechnology, Journal Year: 2024, Volume and Issue: 36(4), P. 042004 - 042004

Published: Oct. 29, 2024

Quantum dot-based optical biosensors represent a significant advancement for detection of mycotoxins that are toxic secondary metabolites produced by fungi and pose serious health risk effects. This review highlights the importance filamentous such as Aspergillus, Penicillium, Fusarium, Claviceps, Alternaria in mycotoxin production, leading to contamination agricultural products subsequent issues. Conventional methods thin-layer chromatography, high-performance liquid gas enzyme-linked immunosorbent assay discussed with their respective advantages limitations. Then innovative use quantum dots (QDs) fabrication is present review, emphasizing unique properties, size-tunable fluorescence high photostability. These properties enable development highly sensitive specific detection. The application QD-based biosensors, based on applied bioreceptors including antibodies, molecularly imprinted polymers aptamer, explored through various strategies recent advancements. concludes underscoring potential providing portable, cost-effective, efficient solutions real-time monitoring enhancing food safety protecting public health.

Language: Английский

Citations

2
Performance parameters as a function of graphene’s chemical potential for SPR biosensor based on 2D materials DOI
Jefte Ceballos-Zumaya, I.A. Sustaita-Torres, J.S. Pérez-Huerta

et al.

Optik, Journal Year: 2024, Volume and Issue: 314, P. 172013 - 172013

Published: Aug. 30, 2024

Language: Английский

Citations

1
Risk Assessment of Microbiological and Chemical Hazards in Foods DOI Creative Commons
Francesco Esposito, Teresa Cirillo

Foods, Journal Year: 2024, Volume and Issue: 13(13), P. 1956 - 1956

Published: June 21, 2024

Food safety constitutes a critical regulatory and quality standard that must be fulfilled by food manufacturers throughout all phases of production [...].

Language: Английский

Citations

0
Sensitive Aflatoxin M1 Detection in Milk by ELISA: Investigation of Different Assay Configurations DOI Creative Commons

Dimitra Kourti,

Μichailia Angelopoulou,

Panagiota Petrou

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(12), P. 515 - 515

Published: Nov. 29, 2024

Aflatoxin M1 (AFM1) exposure through dairy products is associated with adverse health effects, including hepatotoxicity and carcinogenicity. Therefore, the AFM1 presence in milk strictly regulated. In this context, current work focuses on investigation of different competitive enzyme immunoassay configurations for determination high sensitivity short assay duration. Amongst tested, one based incubation anti-AFM1 specific antibody along calibrators/samples a biotinylated conjugate bovine serum albumin (BSA) microwells coated secondary provided six-fold lower detection limit than configuration involving immobilized AFM1-BSA liquid phase antibody. The achieved was 5.0 pg/mL, dynamic range up to 2.0 ng/mL. repeatable intra- inter-assay coefficients variations 3.2% 6.5%, respectively, accurate recovery values from 87.5 108%. Moreover, completed 1.5 h. excellent analytical characteristics analysis time make proposed suitable use by food industry. Furthermore, could be employed enhance immunoassays other low-molecular-weight analytes.

Language: Английский

Citations

0