The Egyptian Journal of Hospital Medicine, Journal Year: 2023, Volume and Issue: 90(2), P. 3395 - 3401
Published: Jan. 1, 2023
Background: Antibiotic resistance is among the major threats to health systems worldwide. often associated with efflux pumps, that distinguish many antibiotics are called Multidrug Resistance (MDR) pumps. Aim: investigate prevalence of Escherichia coli bacteria cause urinary tract infection by identifying gene (pap < /em>E) and pump genes (acrB, TolC , mdfA ) using polymerase chain reaction. Methods: 200 samples were obtained from urine patients infections Baquba Teaching Hospital Al-Batoul Maternity in Diyala Governorate / Iraq during period January April 2022. The nucleic acid DNA bacterial isolates under study was extracted then reaction (PCR) performed through use specialized primers mdfA, TolC) . Results: results antibiotic sensitivity showed all 100% resistant each Ampicillin Augmentin, 98% Amikacin, 96% Tetracycline, 82% Cefepime, 50% (20) contain (mdfA) 100%, 19 out a total 20 isolate targeting 95% both acrB. Conclusion: Most study, isolated (UTIs) multiple (MDR), possessed percentage (100%), while (TolC, acrB found percentage(95%).
Language: Английский