Peptide-based drug discovery: Current status and recent advances

Drug Discovery Today, Journal Year: 2022, Volume and Issue: 28(2), P. 103464 - 103464

Published: Dec. 5, 2022

Language: Английский

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Phage-based delivery systems: engineering, applications, and challenges in nanomedicines

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: June 25, 2024

Abstract Bacteriophages (phages) represent a unique category of viruses with remarkable ability to selectively infect host bacteria, characterized by their assembly from proteins and nucleic acids. Leveraging exceptional biological properties modifiable characteristics, phages emerge as innovative, safe, efficient delivery vectors. The potential drawbacks associated conventional nanocarriers in the realms drug gene include lack cell-specific targeting, cytotoxicity, diminished vivo transfection efficiency. In contrast, engineered phages, when employed cargo vectors, hold promise surmount these limitations attain enhanced efficacy. This review comprehensively outlines current strategies for engineering delineates principal types utilized delivery, explores application phage-based systems disease therapy. Additionally, an incisive analysis is provided, critically examining challenges confronted within domain nanotechnology. primary objective this article furnish theoretical reference that contributes reasoned design development potent systems. Graphical abstract

Language: Английский

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Phage Display as a Medium for Target Therapy Based Drug Discovery, Review and Update

Molecular Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: June 1, 2024

Language: Английский

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Developing recombinant antibodies by phage display technology to neutralize viral infectious diseases

SLAS DISCOVERY, Journal Year: 2024, Volume and Issue: 29(3), P. 100140 - 100140

Published: Jan. 3, 2024

The use of recombinant antibodies developed through phage display technology offers a promising approach for combating viral infectious diseases. By specifically targeting antigens on surfaces, these have the potential to reduce severity infections or even prevent them altogether. With emergence new and more virulent strains viruses, it is crucial develop innovative methods counteract them. Phage has proven successful in generating capable specific antigens, thereby providing powerful tool fight infections. In this mini-review article, we examine development using technology, discuss associated challenges opportunities developing novel treatments Furthermore, provide an overview technology. As continue evolve improve, sophisticated tools based peptide systems are constantly emerging, offering exciting prospects solving scientific, medical, technological problems related diseases near future.

Language: Английский

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Advances of deep Neural Networks (DNNs) in the development of peptide drugs

Future Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 15

Published: Feb. 12, 2025

Peptides are able to bind difficult disease targets with high potency and specificity, providing great opportunities meet unmet medical requirements. Nevertheless, the unique features of peptides, such as their small size, structural flexibility, scarce data availability, bring extra challenges design process. Firstly, this review sums up application peptide drugs in treating diseases. Then, probes into advantages Deep Neural Networks (DNNs) predicting designing structures. DNNs have demonstrated remarkable capabilities prediction, enabling accurate three-dimensional modeling through models like AlphaFold its successors. Finally, deliberates on coping strategies development drugs, along future research directions. Future directions focus further improving accuracy efficiency DNN-based drug design, exploring novel applications accelerating clinical translation. With continuous advancements technology accumulation, poised play an increasingly crucial role field development.

Language: Английский

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Phage therapy for secondary bacterial infections with COVID-19

Current Opinion in Virology, Journal Year: 2021, Volume and Issue: 52, P. 9 - 14

Published: Nov. 9, 2021

Language: Английский

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Preparation of a Nanobody Specific to Dectin 1 and Its Anti-inflammatory Effects on Fungal Keratitis

International Journal of Nanomedicine, Journal Year: 2022, Volume and Issue: Volume 17, P. 537 - 551

Published: Feb. 1, 2022

To prepare a nanobody specific to dectin 1 and verify its specificity anti-inflammatory effects on Aspergillus fumigatus keratitis.The was selected from high-quality shark-antibody library constructed with phage-display technology. The developed in the expression systems of Escherichia coli. Indirect ELISA used determine recombinant protein. potential be recognized expressed surfaces cells corneas detected by immunofluorescence, effect A. keratitis further verified. After infection fumigatus, eyes C57B L/6 mice were treated nanobodies. Human corneal epithelial (HCECs) pretreated nanobodies then incubated fumigatus. Clinical scores slit-lamp photography assess disease response mouse corneas. RT-PCR evaluate mRNA protein IL1β IL6 both HCECs.The successfully through microbial system showed high-affinity binding 1. Furthermore, it exhibited Importantly, reduced clinical compared PBS-treatment group. In addition, decreased infected HCECs stimulated fumigatus.These results suggest that this can bring about effects. This highlights these as innovative therapeutic agents

Language: Английский

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Protein Engineering: Advances in Phage Display for Basic Science and Medical Research

Biochemistry (Moscow), Journal Year: 2022, Volume and Issue: 87(S1), P. S146 - S167

Published: Jan. 1, 2022

Language: Английский

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A single-chain variable fragment antibody exerts anti-nervous necrosis virus activity by irreversible binding

Aquaculture, Journal Year: 2022, Volume and Issue: 552, P. 738001 - 738001

Published: Feb. 5, 2022

Language: Английский

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Advancement in the development of single chain antibodies using phage display technology

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17143 - e17143

Published: April 10, 2024

Phage display technology has become an important research tool in biological research, fundamentally changing the traditional monoclonal antibody preparation process, and been widely used establishment of antigen-antibody libraries, drug design, vaccine pathogen detection, gene therapy, antigenic epitope cellular signal transduction research.The phage is a powerful platform for development. Using technology, single chain fragment variable (scFv) can be screened, replacing disadvantage large size antibodies. libraries have significant implications. Here we describe types antibodies, including chimeric bispecific scFvs. In addition, system, screening specific antibodies by application libraries.

Language: Английский

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