Malaysian Journal of Microbiology,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 1, 2023
The
COVID-19
pandemic
has
caused
a
huge
damage
to
global
society,
not
only
affecting
health
aspect
but
also
economics.The
development
of
effective
vaccines
and
therapeutics
been
critical
step
in
the
fight
against
this
pandemic,
much
research
committed
studying
causative
virus,
SARS-CoV-2.Pseudovirus,
valuable
tool
that
allows
investigating
SARS-CoV-2
safe
controlled
environment,
gained
significant
attention
recent
years.It
is
promising
for
examining
behaviour
viral
envelope
protein
therapeutics.Finding
an
optimized
pseudovirus
system
crucial
various
scientific
medical
applications,
especially
study
infections,
immune
responses
vaccine
development.It
plays
role
advancing
our
understanding
diseases
combat
with
new
variants.This
minreview
focuses
on
concept
systems
factors
yield.
Cell Reports,
Journal Year:
2023,
Volume and Issue:
42(1), P. 111998 - 111998
Published: Jan. 1, 2023
Several
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
Omicron
subvariants
have
recently
emerged,
becoming
the
dominant
circulating
strains
in
many
countries.
These
variants
contain
a
large
number
of
mutations
their
spike
glycoprotein,
raising
concerns
about
vaccine
efficacy.
In
this
study,
we
evaluate
ability
plasma
from
cohort
individuals
that
received
three
doses
mRNA
to
recognize
and
neutralize
these
subvariant
spikes.
We
observed
BA.4/5
BQ.1.1
spikes
are
markedly
less
recognized
neutralized
compared
with
D614G
other
tested.
Also,
who
been
infected
before
or
after
vaccination
present
better
humoral
responses
than
SARS-CoV-2-naive
vaccinated
individuals,
thus
indicating
hybrid
immunity
generates
against
subvariants.
npj Vaccines,
Journal Year:
2025,
Volume and Issue:
10(1)
Published: March 1, 2025
Selecting
a
booster
vaccine
strategy
that
generates
cellular
immune
breadth
is
crucial
for
effectively
recalling
reservoirs
upon
infection
with
severe
acute
respiratory
syndrome
coronavirus-2
(SARS-CoV-2)
variants.
This
post
hoc
analysis
from
multicentre,
randomized
phase
3
study
(CTRI/2022/10/046475)
compared
the
induced
by
self-replicating
mRNA
(samRNA)
GEMCOVAC-OM,
encoding
Omicron
B.1.1.529
Spike
protein,
adenovector
ChAdOx1
nCoV-19,
Wuhan
variant
when
administered
as
booster.
GEMCOVAC-OM
elicited
significant
expansion
of
memory
B-cells
(MBCs)
specific
to
B.1.1.529,
nCoV-19.
also
more
reactive
XBB.1.5
and
BA.2.86
proteins.
Additionally,
triggered
higher
frequencies
Omicron-Spike-specific
T-cells,
including
stem
cell,
central,
effector
subsets.
In
summary,
while
nCoV-19
showed
some
cross-reactivity,
targeted
response.
offers
broader,
longer-lasting
immunity,
making
it
promising
candidate
future
development
global
distribution.
Expert Review of Vaccines,
Journal Year:
2024,
Volume and Issue:
23(1), P. 174 - 185
Published: Jan. 2, 2024
Pseudoviruses
are
recombinant,
replication-incompetent,
viral
particles
designed
to
mimic
the
surface
characteristics
of
native
enveloped
viruses.
They
a
safer,
and
cost-effective
research
alternative
live
With
potential
emergence
next
major
infectious
disease,
more
vaccine
scientists
must
become
familiar
with
pseudovirus
platform
as
development
tool
mitigate
future
outbreaks.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(10), P. 5188 - 5188
Published: May 10, 2024
Since
the
outbreak
of
COVID-19,
researchers
have
been
working
tirelessly
to
discover
effective
ways
combat
coronavirus
infection.
The
use
computational
drug
repurposing
methods
and
molecular
docking
has
instrumental
in
identifying
compounds
that
potential
disrupt
binding
between
spike
glycoprotein
SARS-CoV-2
human
ACE2
(hACE2).
Moreover,
pseudovirus
approach
emerged
as
a
robust
technique
for
investigating
mechanism
virus
attachment
cellular
receptors
screening
targeted
small
molecule
drugs.
Pseudoviruses
are
viral
particles
containing
envelope
proteins,
which
mediate
virus's
entry
with
same
efficiency
live
viruses
but
lacking
pathogenic
genes.
Therefore,
they
represent
safe
alternative
screen
drugs
inhibiting
entry,
especially
highly
enveloped
viruses.
In
this
review,
we
compiled
list
antiviral
plant
extracts
natural
products
extensively
studied
against
emerging
re-emerging
by
technology.
review
is
organized
into
three
parts:
(1)
construction
pseudoviruses
based
on
different
packaging
systems
applications;
(2)
knowledge
viruses;
(3)
active
pseudovirus-mediated
entry.
One
most
crucial
stages
life
cycle
its
penetration
host
cells.
discovery
inhibitors
represents
promising
therapeutic
option
fighting
Materials Chemistry Frontiers,
Journal Year:
2023,
Volume and Issue:
7(18), P. 4063 - 4072
Published: Jan. 1, 2023
At
present,
the
prevalent
Omicron
mutant
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
has
characteristics
high
infectivity
and
strong
concealment,
leading
to
more
asymptomatic
infections.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(14), P. 5407 - 5415
Published: March 13, 2024
COVID-19
vaccines
have
been
provided
to
the
general
public
build
immunity
since
2019
coronavirus
pandemic.
Once
vaccinated,
SARS-CoV-2
neutralizing
antibodies
(NAbs-COVID-19)
are
needed
for
excellent
protection
against
COVID-19.
However,
monitoring
NAbs-COVID-19
is
complicated
and
requires
hospital
visits.
Moreover,
resulting
effective
different
strains
of
depending
on
type
vaccine
received.
Here,
an
overlaid
lateral
flow
immunoassay
(O-LFIA)
was
developed
simultaneous
detection
two
virus
strains,
Delta
Omicron.
The
O-LFIA
visualized
with
T-lines
a
single
device
using
competition
between
free
antigen
antigen-binding
antibody.
Angiotensin-converting
enzyme
2
(ACE2)
immobilized
T-line
binds
remaining
after
antibody
binding.
Under
optimum
conditions,
proposed
exhibited
50%
inhibition
concentrations
(IC50
values)
45.1
53.6
ng/mL
Omicron
variants,
respectively.
Additionally,
platform
applied
real-world
samples
animal
human
serum,
results
that
were
in
good
agreement
those
obtained
standard
method.
In
conclusion,
this
can
be
used
as
alternative
method
detect
enabled
future
advancements
toward
commercialization.
Frontiers in Immunology,
Journal Year:
2023,
Volume and Issue:
14
Published: Oct. 4, 2023
The
SARS-CoV-2
Omicron
variant
has
become
the
dominant
and
exhibits
immune
escape
to
current
COVID-19
vaccines,
further
boosting
strategies
are
required.We
have
conducted
a
non-randomized,
open-label
parallel-controlled
phase
4
trial
evaluate
magnitude
longevity
of
responses
booster
vaccination
with
intramuscular
adenovirus
vectored
vaccine
(Ad5-nCoV),
aerosolized
Ad5-nCoV,
recombinant
protein
subunit
(ZF2001)
or
homologous
inactivated
(CoronaVac)
in
those
who
received
two
doses
vaccines.The
Ad5-nCoV
induced
most
robust
long-lasting
neutralizing
activity
against
IFNg
T-cell
response
among
all
boosters,
distinct
mucosal
response.
SARS-CoV-2-specific
IgA
was
substantially
generated
subjects
boosted
at
day
14
post-vaccination.
At
month
6,
participants
had
remarkably
higher
median
titer
seroconversion
BA.4/5-specific
antibody
than
other
boosters.Our
findings
suggest
that
may
provide
an
efficient
alternative
spread
BA.4/5
variant.https://www.chictr.org.cn/showproj.html?proj=152729,
identifier
ChiCTR2200057278.
Immunity Inflammation and Disease,
Journal Year:
2024,
Volume and Issue:
12(1)
Published: Jan. 1, 2024
Neutralizing
antibodies
(NAbs)
are
an
important
specific
defence
against
viral
infections,
as
these
bind
to
receptor(s)
and
block
the
entry.
NAbs
assessments
therefore
useful
in
determining
individual
or
herd
immunity
SARS-CoV-2.
This
study
aims
deepen
investigation
by
assessing
positivity
rate
of
neutralizing
anti-spike
understand
real
protection
studied
population
