Published: Jan. 1, 2023
During the COVID-19 pandemic, measurement of SARS-CoV-2 RNA levels in wastewater quickly emerged as an additional tool for monitoring and to provide early warning system. This led development several regional, national international projects aimed at applying this approach. The main principle is based on detection viral signature untreated indication infection within connected populations. However, concentration can be impacted by dilution factors or population changes sewer shed, leading misinterpretation results. Therefore, there need normalization ensure accurate representation numbers. aim study was evaluate different bacterial markers their efficiency normalizing WBE data, which will enhance accuracy when interpreting concentrations wastewater. Weekly sampling conducted from two treatment plants (WWTP A WWTP B) eThekwini district over a period three months (July-October 2022). Three biomarkers (crAssphage, Bacteroides (HF 183), Pepper Mild Motile Virus) where chosen ascertain most suitable data normalization. Biomarker SARS CoV-2 samples were determined using droplet digital PCR (ddPCR). Physicochemical characteristics also identify potential impact these biomarkers. To determine biomarker, correlation analysis Adaptive neuro fuzzy inference system (ANFIS) model used. Average sampled WWTPs ranged 0.28 copies/µL 9.57 copies/µL. Among studied, crAssphage recorded highest compared PMMoV HF183 both WWTPs. CrAssphage 7943 (±7.07) 8006 (±4.24) B. 10116 (±120.91) 2474 (±117.37) had 46 84,1 (±5.48) observed Week 1. showed greater association during trend with (0.499) than other A, 183 (-0.191) SARS-CoV 2 (-0.562)). physicochemical electrical conductivity temperature significant biomarker Using ANFIS model, it shown that measured chemical oxygen demand (COD), dissolved (DO), volatile solids (VS). These results indicate possible parameters Furthermore, quantities demonstrated influenced such conductivity, pH temperature. indicates difference influence SARS-CoV-2. all parameters, combined, best crAssphage, COD VS. highlight significance including characteristic studies reliable As study, serve ix efficient surveillance. In addition, has been quantification targets concern, 2, may enhanced combined characteristics, infections.
Language: Английский