Microbial exposure across life reduces susceptibility of Aedes aegypti to Zika virus by enhancing blood digestion and limiting midgut cell infection DOI Creative Commons
William Louie, Ana L. Ramírez,

Rochelle Leung

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 10, 2022

The worldwide expansion of mosquito-borne pathogens necessitates improved control measures, including approaches to reduce transmission by mosquito vectors. Reducing is challenging because determinants vector competence for viruses like Zika (ZIKV) are poorly understood. Our previous work established that Aedes (Ae.) aegypti larvae reared in environmental water containing microbes less susceptible ZIKV as adults compared cohorts laboratory tap with fewer microbial species and lower abundance. Here, we identify a process which environment-derived susceptibility Ae. ZIKV. Provided the midgut represents first barrier infection, hypothesized exposure modulates infection Since mosquitoes live pupae then transition air adults, also define stage life when reduces susceptibility. were treated antibiotics during pupal adult stages, only, or provided no at any stage. Vector was next evaluated ingested ZIKV-spiked bloodmeals. Antibiotic reduced microbiota showed enhanced rates both adults. treatment disrupt resulted increased epithelium permeability, higher numbers ZIKV-infected cells, impaired bloodmeal digestion. Parallel experiments antibiotic-treated gnotobiotic dysbiotic state created antibiotic use does not influence permeability more than bacterial responsible reducing competence. disrupted via bloodmeals expression genes associated digestion metabolism relative whose antibiotics. Together, these data show throughout restricts facilitating blood cell infection. Understanding connections between microbiota, physiology, arbovirus can lead novel decrease will improve understanding habitats microbes. Author Summary Mosquito-transmitted continue threaten human health. Absent vaccines treatments, controlling limiting their ability transmit primary way prevent viral diseases. role play shaping virus has been limited association-based studies. prior develop bacteria bacteria. Here explains this association. aquatic terrestrial need be present We used water-derived only stages observed disruption increases impairs These findings advance microbiota-mosquito-virus interactions further implicate means restrict mosquitoes.

Language: Английский

"Symbiotic secrets: exploring the gut microbiome of Aedes aegypti larvae and its role in mosquito physiology" DOI

C. F. Sini Francis,

Honey Sebastian, Ambalaparambil Vasu Sudhikumar

et al.

International Journal of Tropical Insect Science, Journal Year: 2024, Volume and Issue: 44(4), P. 1591 - 1607

Published: May 20, 2024

Language: Английский

Citations

0
Larval crowding enhances dengue virus loads in Aedes aegypti, a relationship that might increase transmission in urban environments DOI Creative Commons
Heverton Leandro Carneiro Dutra, Dustin J. Marshall, Belinda Comerford

et al.

PLoS neglected tropical diseases, Journal Year: 2024, Volume and Issue: 18(9), P. e0012482 - e0012482

Published: Sept. 10, 2024

Background Climate change and urbanization will alter the global distribution of disease vectors, changing burden in yet unpredictable ways. Aedes aegypti is a mosquito responsible for transmitting dengue, Zika, chikungunya, yellow fever viruses that breeds containers associated with urban environments. We sought to understand how ambient temperature larval densities immature aquatic phases determine adult life history traits dengue virus loads post-infection. predicted crowding high temperatures would both lead smaller mosquitoes might struggle invest an immune response and, hence, exhibit viral loads. Methods first examined from rural areas via meta-analysis. then used these data inform laboratory-based 2x2 design examining interacting effects (21 vs. 26°C) density (0.2 0.4 larvae/mL) on Results found had ~8-fold increase compared more sites. In lab, we impact than temperature. Crowding led slower development, mosquitoes, less survival, lower fecundity, higher loads, as predicted. The faster reduced virus-reducing effect rearing was, however, overwhelmed by when factors were present. Conclusions These reveal complex interactions between environmental experienced traits. They especially highlight importance respect Together, suggest environments enhance therefore, possibly transmission, concerning result given increasing rates globally.

Language: Английский

Citations

0
Endophytic Fungus Alternaria Macrospora: A Promising and Eco-Friendly Source for Controlling Aedes Aegypti Larvae and for Bactericidal Use DOI
Chinnasamy Ragavendran, Chinnaperumal Kamaraj,

Devarajan Natarajan

et al.

Published: Jan. 1, 2023

The present study assesses the metabolites of endophytic fungal isolates’ larvicidal and pupicidal activity on Aedes aegypti mosquito larvae. isolates were confirmed by macroscopic, microscopic, PCR using a universal internal transcribed spacer primer for molecular identification isolated fungus at species level. was first tested its ability to produce toxic compounds in A. aegpti larvae 1-4th instar. secondary evaluated bioassays different test concentrations (100-500 µg/mL) each instar determined 1st 4th exposed ethyl acetate extracts, which exhibited lethal that killed 50% (LC50 = 96.167, 131.370, 217.595, 143.864 µg/mL 90% (LC90 395.16, 483.692, 882.545, 655.722 µg/mL). After 120 h exposure, ovicidal bioassay’s mean (%) observed. A concentration-dependent pattern found enzymatic level acetylcholinesterase mycelial metabolites. Treating 500 mycelia, produced significant histopathological damage highest growth inhibition zones observed during antibacterial investigation Alternaria macrospora against E. coli (22 mm) S. aureus (20 mm). Also, detected mycelium docking studies identify their binding towards FimH tyrosyl-RNA synthetase protein. with further in-vivo zebrafish model evaluate developmental toxicity. This is report species. findings demonstrate might be an ideal, cheap, small, environmentally suitable source controlling

Language: Английский

Citations

0
Establishment and comparative genomics of a high-quality collection of mosquito-associated bacterial isolates - MosAIC (Mosquito-Associated Isolate Collection) DOI Creative Commons
Aidan Foo, Laura E. Brettell, Holly L. Nichols

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 4, 2023

Abstract Mosquitoes transmit medically important human pathogens, including viruses like dengue virus and parasites such as Plasmodium spp., the causative agent of malaria. Mosquito microbiomes are critically for ability mosquitoes to disease-causing agents. However, while large collections bacterial isolates genomic data exist vertebrate microbiomes, vast majority work in date is based on 16S rRNA gene amplicon that provides limited taxonomic resolution no functional information. To address this gap facilitate future studies using experimental microbiome manipulations, we generated a Mos quito- A ssociated Isolate C ollection (MosAIC) consisting 392 with extensive metadata high-quality draft genome assemblies publicly available use by scientific community. MosAIC encompasses 142 species spanning 29 families, members Enterobacteriaceae comprising 40% collection. Phylogenomic analysis three genera, Enterobacter, Serratia , Elizabethkingia reveal lineages mosquito-associated bacteria isolated from different mosquito multiple laboratories. Investigation into species’ pangenomes further reveals clusters genes specific these lineages, which interest identify functions underlying host association. Altogether, describe generation physical collection isolates, their data, analyses selected groups context closely related providing unique, highly valuable resource investigate factors colonisation adaptation within hosts. Future efforts will expand include broader geographic representation, especially individuals collected field populations, well other microbes, fungi, archaea, protozoa.

Language: Английский

Citations

0
EVALUATING THE EFFICACY OF COMMERCIAL Bacillus thuringiensis israelensis FOR Aedes albopictus LARVAL CONTROL IN VARIOUS LAND USE DOI Open Access
Othman Wan-Norafikah,

Zaifol Atiqah-Izzah,

C.D. Chen

et al.

Serangga, Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 1, 2023

A commercial bacterial product of Bacillus thuringiensis israelensis (Bti); Mosquito BTI from USA was tested for its efficacy in exterminating the local populations Aedes albopictus larvae. Larval surveys were carried out to obtain field Ae. various land use namely dengue-risk and dengue-free housing localities as well agrarian like oil palm plantations, rubber estates paddy fields. 24-hour larval bioassay using performed on each population. Only a maximum 2.00% mortality observed by end first four hours exposure while 25.00% displayed at 24 exposure. Results showed potential Bti reducing but more than may be required achieve complete mortalities among these upon Bti. Some limitations this study also discussed.

Language: Английский

Citations

0
Microbial exposure across life reduces susceptibility of Aedes aegypti to Zika virus by enhancing blood digestion and limiting midgut cell infection DOI Creative Commons
William Louie, Ana L. Ramírez,

Rochelle Leung

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 10, 2022

The worldwide expansion of mosquito-borne pathogens necessitates improved control measures, including approaches to reduce transmission by mosquito vectors. Reducing is challenging because determinants vector competence for viruses like Zika (ZIKV) are poorly understood. Our previous work established that Aedes (Ae.) aegypti larvae reared in environmental water containing microbes less susceptible ZIKV as adults compared cohorts laboratory tap with fewer microbial species and lower abundance. Here, we identify a process which environment-derived susceptibility Ae. ZIKV. Provided the midgut represents first barrier infection, hypothesized exposure modulates infection Since mosquitoes live pupae then transition air adults, also define stage life when reduces susceptibility. were treated antibiotics during pupal adult stages, only, or provided no at any stage. Vector was next evaluated ingested ZIKV-spiked bloodmeals. Antibiotic reduced microbiota showed enhanced rates both adults. treatment disrupt resulted increased epithelium permeability, higher numbers ZIKV-infected cells, impaired bloodmeal digestion. Parallel experiments antibiotic-treated gnotobiotic dysbiotic state created antibiotic use does not influence permeability more than bacterial responsible reducing competence. disrupted via bloodmeals expression genes associated digestion metabolism relative whose antibiotics. Together, these data show throughout restricts facilitating blood cell infection. Understanding connections between microbiota, physiology, arbovirus can lead novel decrease will improve understanding habitats microbes. Author Summary Mosquito-transmitted continue threaten human health. Absent vaccines treatments, controlling limiting their ability transmit primary way prevent viral diseases. role play shaping virus has been limited association-based studies. prior develop bacteria bacteria. Here explains this association. aquatic terrestrial need be present We used water-derived only stages observed disruption increases impairs These findings advance microbiota-mosquito-virus interactions further implicate means restrict mosquitoes.

Language: Английский

Citations

0