The Antiviral Activity of the Lectin Griffithsin against SARS-CoV-2 Is Enhanced by the Presence of Structural Proteins DOI Creative Commons
Arjan Bains,

Kathryn Fischer,

Wenyan Guan

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(12), P. 2452 - 2452

Published: Dec. 18, 2023

Although COVID-19 transmission has been reduced by the advent of vaccinations and a variety rapid monitoring techniques, SARS-CoV-2 virus itself shown remarkable ability to mutate persist. With this long track record immune escape, researchers are still exploring prophylactic treatments curtail future variants. Specifically, much focus placed on antiviral lectin Griffithsin in preventing spike protein-mediated infection via hACE2 receptor (direct infection). However, an oft-overlooked aspect is viral capture attachment receptors such as DC-SIGN, which thought facilitate initial stages lung tissue (called trans-infection). In addition, while escape dictated mutations protein, coronaviral virions also incorporate M, N, E structural proteins within particle. paper, we explored how several facets both virion can affect attenuate infectivity pseudovirus. We found that was better inhibitor hACE2-mediated direct when M protein present compared it absent (possibly providing explanation regarding why shows inhibition against authentic opposed pseudotyped viruses, generally do not contain M) effective DC-SIGN-mediated trans-infection. Furthermore, DC-SIGN appeared mediate trans-infection exclusively binding with no significant effect observed other (M, and/or E) were present. These results provide etiological data may help development novel treatments, either leveraging strategy prevent or narrowing efforts inhibit protein.

Language: Английский

Evolution and Impact of Nucleic Acid Amplification Test (NAAT) for Diagnosis of Coronavirus Disease DOI Creative Commons

Sumbul Fatma Khan,

Priyanka Rathod, Vivek Kumar Gupta

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(20), P. 8124 - 8146

Published: April 30, 2024

ADVERTISEMENT RETURN TO ARTICLES ASAPReviewNEXTEvolution and Impact of Nucleic Acid Amplification Test (NAAT) for Diagnosis Coronavirus DiseaseSumbul Fatma KhanSumbul KhanDepartment Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, 440033, MS IndiaMore by Sumbul KhanView BiographyView Biography, Priyanka RathodPriyanka RathodDepartment Rathod, Vivek K. GuptaVivek GuptaDepartment Biochemistry, National JALMA Institute Leprosy & Other Mycobacterial Diseases (ICMR), Agra -282004, GuptaView Pramod B. KhedekarPramod KhedekarDepartment KhedekarView Rupesh V. Chikhale*Rupesh ChikhaleUCL School Pharmacy, Department Biological Chemistry, University College London, London WC1N 1AX, United Kingdom*Email: [email protected]More ChikhaleView Biographyhttps://orcid.org/0000-0001-5622-3981Cite this: Anal. Chem. 2024, XXXX, XXX, XXX-XXXPublication Date (Web):April 30, 2024Publication History Received18 November 2023Accepted15 April 2024Revised11 2024Published online30 2024https://doi.org/10.1021/acs.analchem.3c05225© 2024 The Authors. Published American Chemical Society. This publication is licensed under CC-BY 4.0. License Summary*You are free to share (copy redistribute) this article in any medium or format adapt (remix, transform, build upon) the material purpose, even commercially within parameters below:Creative Commons (CC): a Creative license.Attribution (BY): Credit must be given creator.View full license*DisclaimerThis summary highlights only some key features terms actual license. It not license has no legal value. Carefully review before using these materials. Open Access indicated. Learn MoreArticle Views-Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views COUNTER-compliant sum text downloads since 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score quantitative measure attention that research received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail (5 MB) Get e-AlertscloseSUBJECTS:Fluorescence,Genetics,Peptides proteins,Probes,SARS-CoV-2 e-Alerts

Language: Английский

Citations

6
Deubiquitinase USP39 promotes SARS-CoV-2 replication by deubiquitinating and stabilizing the envelope protein DOI Creative Commons
Xiang Chen, Tian Li,

Linran Zhang

et al.

Antiviral Research, Journal Year: 2023, Volume and Issue: 221, P. 105790 - 105790

Published: Dec. 27, 2023

The SARS-CoV-2 envelope (E) protein is highly conserved among different viral variants and important for assembly production. Our recent study found that the E ubiquitinated degraded by E3 ligase RNF5 through proteasome pathway. However, whether ubiquitination can be reversed host deubiquitinase has not yet been determined. Here, we identify mass spectrum analysis deubiquitinases USP14 USP39 specifically interact with E, while potently reverses polyubiquitination. interacts via arginine-rich motif (AR) deubiquitinates polyubiquitination inactive ubiquitin-specific protease domain. Therefore, protects from RNF5-mediated degradation, resulting in enhancement of stability E-induced cytokine storms. Moreover, loss-and-gain assays demonstrated promotes replication various strains stabilizing level but other proteins. findings provide useful targets development novel anti-SARS-CoV-2 strategies.

Language: Английский

Citations

11
Main and papain-like proteases as prospective targets for pharmacological treatment of coronavirus SARS-CoV-2 DOI Creative Commons

Larysa V. Yevsieieva,

Kateryna Lohachova, Alexander Kyrychenko

et al.

RSC Advances, Journal Year: 2023, Volume and Issue: 13(50), P. 35500 - 35524

Published: Jan. 1, 2023

The pandemic caused by the coronavirus SARS-CoV-2 led to a global crisis in world healthcare system. Despite some progress creation of antiviral vaccines and mass vaccination population, number patients continues grow because spread new mutations. There is an urgent need for direct-acting drugs capable suppressing or stopping main mechanisms reproduction SARS-CoV-2. Several studies have shown that successful replication virus cell requires proteolytic cleavage protein structures virus. Two proteases are crucial replicating other coronaviruses: protease (Mpro) papain-like (PLpro). In this review, we summarize essential viral proteins required its life cycle as targets chemotherapy infection provide critical summary development against COVID-19 from drug repurposing strategy up molecular design novel covalent non-covalent agents inhibiting replication. We overview choice Mpro PLpro promising pharmacological impact on cycle.

Language: Английский

Citations

10
Design, Synthesis, and Biological Evaluation of 1,2,4-Oxadiazole Derivatives Containing an Aryl Carboxylic Acid Moiety as Potent Sarbecovirus Papain-like Protease Inhibitors DOI
Bo Qin,

Chengwei Wu,

Binbin Zhao

et al.

Journal of Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 67(12), P. 10211 - 10232

Published: June 13, 2024

Papain-like protease (PLpro) is a promising therapeutic target for its pivotal role in the life cycle of SARS-CoV-2. A series 1,2,4-oxadiazole derivatives was designed and synthesized via ring formation strategy based on SARS-CoV-2 PLpro-GRL0617 complex structure. Systematic structure-activity relationship studies revealed that introducing oxadiazole aryl carboxylic acid moieties to GRL0617 enhanced enzymatic inhibition activity, affinity, deubiquitination capacity toward PLpro. 1,2,4-Oxadiazole compounds

Language: Английский

Citations

3
Multifaceted role of SARS-CoV-2 structural proteins in lung injury DOI Creative Commons
Guoping Zheng,

Guanguan Qiu,

Huifeng Qian

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 5, 2024

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human to cause distress (ARDS) and contains four structural proteins: spike, envelope, membrane, nucleocapsid. An increasing number of studies have demonstrated that all proteins SARS-CoV-2 are capable causing lung injury, even without presence intact virus. Therefore, topic protein-evoked injury warrants more attention. In current article, we first synopsize features proteins. Second, discuss mechanisms for protein-induced inflammatory responses in vitro . Finally, list findings indicate themselves toxic sufficient induce vivo Recognizing triggered by may facilitate development targeted modalities treating COVID-19.

Language: Английский

Citations

2
Stability of Envelope Proteins of SARS-CoV-2 Variants Could Be a Choice of New Drug Target DOI Creative Commons
Ambreen Shafaat Khan, Bennet Angel, Annette Angel

et al.

International Journal of experimental research and review, Journal Year: 2024, Volume and Issue: 42, P. 111 - 119

Published: Aug. 30, 2024

Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has emerged as one of the worst viral pandemics during past few years. As reported by World Health Organization, around 77,56 49 520 cases and 70 51 720 deaths were from all over world, which includes approximately 3500 patients recent only. Currently, circulating variants SARS-CoV-2 are KP.3, JN.1, BA.2.86 KP.2. Mass vaccinations have been provided since end December 2020, led to 5.47 billion people vaccinated till date. However, disease continues in small foci world. Development an effective drug target mutation independent vaccine thus becomes essential research priorities. Owing unavailability a specific molecule, present study focused on development treat COVID-19. In-house primers designed for four structural genes viz., Spike protein, ORF1ab, Nucleocapsid gene, Envelope gene. Samples different waves amplified using these employing Polymerase Chain Reaction (PCR) assay. A total number 86 RT PCR positive samples studied, results showed most frequent appearance (80.2%) (E) protein samples. This suggests that transmission across numbers human hosts, it is was stable one. The severe Delta variant presence E proteins assayed. Blocking new intervene intracellular replication virus could be strategy.

Language: Английский

Citations

2
Advances in Understanding the Envelope Protein in Coronavirus Infection DOI Open Access
Aakanksha Agarwal, Ashley L. Steed

Journal of Immunological Sciences, Journal Year: 2024, Volume and Issue: 8(1), P. 1 - 7

Published: May 7, 2024

The COVID-19 pandemic continues to impart devastating effects on human health, healthcare systems, and the economy. Vaccination, monoclonal antibodies, antiviral therapies prevent limit early infection. Unfortunately, few strategies exist mitigate disease burden in vast number of individuals who seek medical attention with established infection severe disease. While we have a limited understanding mechanistic basis by which SARS-CoV-2 causes critical illness, increasing evidence suggests that host-pathogen interactions shape immune responses drive pathogenesis COVID-19. Therefore, it is imperative understand roles viral proteins how they course One interesting protein envelope (E) SARS-CoV-2; this tiny structural has been implicated many phases life cycle. Importantly, E facilitates packaging replication, its deletion reduces pathogenicity. also possesses ion channel functions, interacts host proteins, potential various topologies. This review aims establish an updated highlighting recent developments investigation protein, particularly comparison SARS-CoV. thorough knowledge will enable targeted studies hopes tailored efficacious treatments.

Language: Английский

Citations

1
Decoding the intricacies: a comprehensive analysis of microRNAs in the pathogenesis, diagnosis, prognosis and therapeutic strategies for COVID-19 DOI Creative Commons
Shukur Wasman Smail,

Sarah Mousa Hirmiz,

Akhter Ahmed Ahmed

et al.

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 7, 2024

The pandemic of coronavirus disease-19 (COVID-19), provoked by the appearance a novel named severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), required worldwide healthcare emergency. This has elicited an immediate need for accelerated research into its mechanisms disease, criteria diagnosis, methods forecasting outcomes, and treatment approaches. microRNAs (miRNAs), are diminutive RNA molecules, that non-coding participate in gene expression regulation post-transcriptionally, having important participation regulating immune processes. miRNAs have granted substantial interest their impact on viral replication, cell proliferation, modulation how host’s system responds. narrative review delves host miRNAs’ multifaceted roles within COVID-19 context, highlighting involvement disease progression, diagnostics, prognostics aspects, given stability biological fluids varied profiles when responding to infection. Additionally, we discuss complicated interactions between SARS-CoV-2 cellular machinery facilitated revealing dysregulation miRNA advances evasion, inflammatory responses. Furthermore, it investigates potential as therapeutic agents, whether synthetic or naturally occurring, which could be harnessed either mitigate harmful inflammation enhance antiviral However, searching more deeply is needed clarify involved pathogenesis COVID-19, diagnosis processes, prognostic assessments, approaches patients.

Language: Английский

Citations

1
Developing inhibitory peptides against SARS-CoV-2 envelope protein DOI Creative Commons
Ramsey Bekdash, Kazushige Yoshida, Manoj S. Nair

et al.

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(3), P. e3002522 - e3002522

Published: March 14, 2024

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has affected approximately 800 million people since the start of Disease 2019 (COVID-19) pandemic. Because high rate mutagenesis in SARS-CoV-2, it is difficult to develop a sustainable approach for prevention and treatment. The Envelope (E) protein highly conserved among human coronaviruses. Previous studies reported that SARS-CoV-1 E deficiency reduced viral propagation, suggesting inhibition might be an effective therapeutic strategy SARS-CoV-2. Here, we report inhibitory peptides against SARS-CoV-2 named iPep-SARS2-E. Leveraging E-induced alterations proton homeostasis NFAT/AP-1 pathway mammalian cells, developed screening platforms design optimize bind inhibit protein. Using Vero-E6 human-induced pluripotent stem cell-derived branching lung organoid mouse models with found iPep-SARS2-E significantly inhibits virus egress reduces cytotoxicity propagation vitro vivo. Furthermore, peptide can customizable other coronaviruses such as Middle East (MERS-CoV). results indicate potential target

Language: Английский

Citations

1
COVID-19: Perspectives on innate immune evasion DOI
Alaa A. A. Aljabali, Mohamed El‐Tanani, Debmalya Barh

et al.

Progress in molecular biology and translational science, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

0