Novel Core Gene Signature Associated with Inflammation-to-Metaplasia Transition in Influenza A Virus-Infected Lungs DOI Open Access
Innokenty A. Savin,

Aleksandra V. Sen’kova,

Е. П. Гончарова

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 11958 - 11958

Published: Nov. 7, 2024

Respiratory infections caused by RNA viruses are a major contributor to respiratory disease due their ability cause annual epidemics with profound public health implications. Influenza A virus (IAV) infection can affect variety of host signaling pathways that initiate tissue regeneration hyperplastic and/or dysplastic changes in the lungs. Although these involved lung recovery after IAV infection, some cases, they lead serious failure. Despite being ubiquitously observed, there limited data on regulation long-term from leading normal or repair represented inflammation-to-metaplasia transition mice humans. To address this knowledge gap, we used integrative bioinformatics analysis further verification vivo elucidate dynamic molecular IAV-infected murine and identified core genes (

Language: Английский

Defective viral genomes: advances in understanding their generation, function, and impact on infection outcomes DOI Creative Commons
Justin W. Brennan, Yan Sun

mBio, Journal Year: 2024, Volume and Issue: 15(5)

Published: April 3, 2024

ABSTRACT Defective viral genomes (DVGs) are truncated derivatives of their parental generated during an aberrant round genomic replication. Distinct classes DVGs have been identified in most families both positive- and negative-sense RNA viruses. Importantly, detected clinical samples from virally infected individuals emerging body association studies implicates shaping the severity disease caused by infections humans. Consequently, there is growing interest understanding molecular mechanisms de novo DVG generation, how interact with innate immune system, harnessing as novel therapeutics vaccine adjuvants to attenuate pathogenesis. This minireview focuses on single-stranded viruses (excluding retroviridae), summarizes current knowledge functions diversity species, roles play influencing progression, application antivirals adjuvants.

Language: Английский

Citations

10
Exploring the mechanisms of interference, persistence and antiviral potential of defective interfering particles DOI Creative Commons
Mousumi Bora,

M. Manu,

Monu Karki

et al.

Discover Viruses., Journal Year: 2025, Volume and Issue: 2(1)

Published: Jan. 17, 2025

Defective interfering particles (DIPs) were first described by Preben von Magnus as a non-infectious form of influenza virus arising due to undiluted passages the in embryonated eggs. DIPs are characterized internal deletions viral genome, interference with standard replication, attenuation cytopathic effects and persistence. Even though DIP-mediated host cell persistence have been reported both experimental natural infections, processes involved modifying or influencing fate infection poorly understood. In this review, properties DIPs, mechanisms modulation disease outcomes their possibilities towards an antiviral approach highlighted.

Language: Английский

Citations

0
Antivirotics based on defective interfering particles: emerging concepts and challenges DOI Creative Commons

S. V. Maryanchik,

S. E. Borovikova,

Anna O. Ivanova

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 24, 2025

Viruses are obligate parasites, that use the host's internal metabolic systems for their own reproduction. This complicates search molecular targets to prevent spread of viral infection without disrupting vital functions human cells. Defective interfering particles (DIPs) natural competitors viruses important resources DIPs emerge during infection, originate from normal replication process and inhibit its progression, making them an interesting candidate antiviral therapy. Here we describe biology DIPs, advances in DIP-based technology, analyze therapeutic potential provide a systemic overview existing preventive strategies.

Language: Английский

Citations

0
Mathematical model calibrated to in vitro data predicts mechanisms of antiviral action of the influenza defective interfering particle “OP7” DOI Creative Commons
Daniel Rüdiger, Julita Piasecka,

Jan Küchler

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(4), P. 109421 - 109421

Published: March 5, 2024

Defective interfering particles (DIPs) are regarded as potent broad-spectrum antivirals. We developed a mathematical model that describes intracellular co-infection dynamics of influenza standard virus (STV) and "OP7", new type DIP discovered recently. Based on experimental data from

Language: Английский

Citations

1
Harnessing defective interfering particles and lipid nanoparticles for effective delivery of an anti-dengue virus RNA therapy DOI Creative Commons
Min‐Hsuan Lin,

Pramila Maniam,

Dongsheng Li

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2024, Volume and Issue: 36(1), P. 102424 - 102424

Published: Dec. 13, 2024

Language: Английский

Citations

1
Generation of “OP7 chimera” defective interfering influenza A particle preparations free of infectious virus that show antiviral efficacy in mice DOI Creative Commons

Tanya Dogra,

Lars Pelz, Julia D. Boehme

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Nov. 28, 2023

Influenza A virus (IAV) defective interfering particles (DIPs) are considered as new promising antiviral agents. Conventional DIPs (cDIPs) contain a deletion in the genome and can only replicate upon co-infection with infectious standard (STV), during which they suppress STV replication. We previously discovered type of IAV DIP "OP7" that entails genomic point mutations displays higher efficacy than cDIPs. To avoid safety concerns for medical use OP7 preparations, we developed production system does not depend on IAV. reconstituted mixture consisting cDIPs chimera DIPs, both harbor their genome. complement defect, deleted viral protein is expressed by suspension cell line used shake flasks. Here, preparations harvested contaminated virions, fraction depended multiplicity infection. Intranasal administration material was well tolerated mice. rescue from an otherwise lethal infection no signs disease demonstrated remarkable efficacy. The clinical development this class broad-spectrum may contribute to pandemic preparedness.

Language: Английский

Citations

3
Production of antiviral “OP7 chimera” defective interfering particles free of infectious virus DOI Creative Commons
Lars Pelz,

Tanya Dogra,

Pavel Marichal‐Gallardo

et al.

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Jan. 13, 2024

Abstract Defective interfering particles (DIPs) of influenza A virus (IAV) are suggested for use as broad-spectrum antivirals. We discovered a new type IAV DIP named “OP7” that carries point mutations in its genome segment (Seg) 7 instead deletion conventional DIPs (cDIPs). Recently, using genetic engineering tools, we generated “OP7 chimera DIPs” carry Seg plus 1. Together with cDIPs, OP7 were produced shake flasks the absence infectious standard (STV), rendering UV inactivation unnecessary. However, only part virions harvested (78.7%) and total titers relatively low. Here, describe establishment an production process applicable large-scale production. To increase titers, reduced temperature from 37 to 32 °C during replication. Production almost pure preparations (99.7%) was achieved high titer 3.24 log 10 (HAU/100 µL). This corresponded 11-fold relative initial process. Next, this transferred stirred tank bioreactor resulting comparable yields. Moreover, harvests purified concentrated by steric exclusion chromatography displayed increased efficacy vitro. Finally, perfusion rate control established, 79-fold yields compared original batch flasks. Again, very purity obtained. could thus be excellent starting good manufacturing practice Key points • Scalable cell culture-based highly effective antiviral Perfusion mode purification train results

Language: Английский

Citations

0
Respiratory Pathogen Dynamics in Community Fever Cases — Jiangsu Province, China (2023-2024) DOI
Fēi Dèng,

Zhuhan Dong,

Tian Qiu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 23, 2024

Abstract Background Respiratory infectious disease was the world's highest incidence of diseases, it caused by a variety respiratory pathogens, and current monitoring pathogens in world focused on influenza coronavirus. This study aimed to establish pathogen spectrum local acute infections further co-infection pathogens. Time series models commonly used predict diseases can effectively outbreaks serve as auxiliary tools for surveillance response strategy formulation. Methods From June 2023 February 2024, we collected influenza-like illness (ILI) cases weekly from community Xuanwu District, Nanjing, obtained total 2,046 samples. We established Nanjing analyzed age distribution symptom counts associated with various compared age, gender, counts, viral loads between individuals co-infections those single infections. An autoregressive comprehensive moving average model (ARIMA) constructed diseases. Results Among 2046 samples, detection rate nucleic acids 53.57% (1096/2046), A virus 503 (24.00%), B 224 (10.95%), HCoV 95 (4.64%) being predominant. Some were statistically significant number symptoms. The positive mixed 6.11% (125/2046), There no difference symptoms simple infection. After multiple iterative analyses, an ARIMA (0,1,4), (0,0,0) optimal model, R2 value 0.930, indicating good predictive performance. Conclusions In past, Jiangsu Province complex, main groups different viruses different, causing symptoms, had correlation gender patients. provided estimate future incidence, which plateaued subsequent months.

Language: Английский

Citations

0
Respiratory pathogen dynamics in community fever cases: Jiangsu Province, China (2023–2024) DOI Creative Commons
Fēi Dèng,

Zhuhan Dong,

Tian Qiu

et al.

Virology Journal, Journal Year: 2024, Volume and Issue: 21(1)

Published: Sept. 20, 2024

Respiratory infectious diseases have the highest incidence among worldwide. Currently, global monitoring of respiratory pathogens primarily focuses on influenza and coronaviruses. This study included other common to establish a local pathogen spectrum. We investigated analyzed co-infection patterns these explored impact lifting non-pharmaceutical interventions (NPIs) transmission pathogens. Additionally, we used predictive model for diseases, utilizing commonly An autoregressive comprehensive moving average (ARIMA), which can effectively forecast disease incidence.

Language: Английский

Citations

0
Exploring the treatment of SARS-CoV-2 with modified vesicular stomatitis virus DOI

Nishnath Polavarapu,

Madison Doty,

Hana M. Dobrovolny

et al.

Journal of Theoretical Biology, Journal Year: 2024, Volume and Issue: unknown, P. 111959 - 111959

Published: Oct. 1, 2024

Language: Английский

Citations

0