Development of novel monoclonal antibodies for blocking NF-κB activation induced by CD2v protein in African swine fever virus DOI Creative Commons
Rongrong Fan,

Zeliang Wei,

Mengmeng Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: May 23, 2024

CD2v, a critical outer envelope glycoprotein of the African swine fever virus (ASFV), plays central role in hemadsorption phenomenon during ASFV infection and is recognized as an essential immunoprotective protein. Monoclonal antibodies (mAbs) targeting CD2v have demonstrated promise both diagnosing combating (ASF). The objective this study was to develop specific monoclonal against CD2v. In investigation, Recombinant expressed eukaryotic cells, murine mAbs were generated through meticulous screening hybridoma cloning. Various techniques, including indirect enzyme-linked immunosorbent assay (ELISA), western blotting, immunofluorescence (IFA), bio-layer interferometry (BLI), employed characterize mAbs. Epitope mapping conducted using truncation mutants epitope peptide mapping. An optimal antibody pair for highly sensitive sandwich ELISA identified, antigenic structures by elucidated. Two linear epitopes conserved genotype II strains, particularly Chinese endemic along with unique glycosylated epitope. Three mAbs, 2B25, 3G25, 8G1, effectively blocked CD2v-induced NF-κB activation. This provides valuable insights into structure obtained hold great potential use development ASF diagnostic strategies, identified may contribute vaccine ASFV.

Language: Английский

ASFV Subunit Vaccines: Strategies and Prospects for Future Development DOI
Yuchen Zhang,

Xiuzhen Mei,

Chao Zhang

et al.

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 197, P. 107063 - 107063

Published: Oct. 21, 2024

Language: Английский

Citations

1
Development of novel monoclonal antibodies for blocking NF-κB activation induced by CD2v protein in African swine fever virus DOI Creative Commons
Rongrong Fan,

Zeliang Wei,

Mengmeng Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: May 23, 2024

CD2v, a critical outer envelope glycoprotein of the African swine fever virus (ASFV), plays central role in hemadsorption phenomenon during ASFV infection and is recognized as an essential immunoprotective protein. Monoclonal antibodies (mAbs) targeting CD2v have demonstrated promise both diagnosing combating (ASF). The objective this study was to develop specific monoclonal against CD2v. In investigation, Recombinant expressed eukaryotic cells, murine mAbs were generated through meticulous screening hybridoma cloning. Various techniques, including indirect enzyme-linked immunosorbent assay (ELISA), western blotting, immunofluorescence (IFA), bio-layer interferometry (BLI), employed characterize mAbs. Epitope mapping conducted using truncation mutants epitope peptide mapping. An optimal antibody pair for highly sensitive sandwich ELISA identified, antigenic structures by elucidated. Two linear epitopes conserved genotype II strains, particularly Chinese endemic along with unique glycosylated epitope. Three mAbs, 2B25, 3G25, 8G1, effectively blocked CD2v-induced NF-κB activation. This provides valuable insights into structure obtained hold great potential use development ASF diagnostic strategies, identified may contribute vaccine ASFV.

Language: Английский

Citations

1