Translocation of the Alphanucleorhabdovirus X proteins from the cytoplasm into the nucleus through interaction with nucleocapsid protein is essential for viral pathogenesis DOI Creative Commons
Shuo Wang, Shuang Ni,

Huanhuan Lou

et al.

Phytopathology Research, Journal Year: 2024, Volume and Issue: 6(1)

Published: Nov. 19, 2024

Abstract The diverse rhabdoviruses infecting plants and animals have conserved genome organizations, the functions of viral structural proteins been extensively studied. However, increasing number found to encode various accessory proteins, whose specific roles during infection remain poorly understood. In this study, we investigated function X encoded by several members genus Alphanucleorhabdovirus . Using recently established eggplant mottled dwarf virus (EMDV) reverse genetics system, that recombinant EMDV lacking gene was able systemically infect Nicotiana benthamiana plants, albeit with reduced efficiency. deletion mutant largely restricted veinal tissues caused asymptomatic infections. protein, which localized cytoplasm when expressed alone, translocated nucleus via a interaction nucleocapsid (N) protein. Through analyzing interactions mutants phenotypes derived mutants, demonstrated carboxyl-terminal region protein (amino acids 71–83) is crucial for its N pathogenesis. Moreover, related alphanucleorhabdoviruses could fully or partially complement in infection. These findings provide new insights into plant rhabdovirus

Language: Английский

Identification and complete genome sequence of honeysuckle-associated cytorhabdovirus in China DOI
Jing Yang, Liming Liu, Junhui Li

et al.

Archives of Virology, Journal Year: 2025, Volume and Issue: 170(3)

Published: Feb. 20, 2025

Language: Английский

Citations

0
Unveiling the genetic diversity of the genera Enamovirus and Polerovirus through data-driven virus discovery DOI
V. Kavi Sidharthan,

Vijay Prakash Reddy,

K. Nagendran

et al.

Archives of Virology, Journal Year: 2025, Volume and Issue: 170(4)

Published: March 13, 2025

Language: Английский

Citations

0
Trirhavirus: the first genus of tripartite viruses in the family Rhabdoviridae DOI
Nicolás Bejerman, Humberto Debat

Archives of Virology, Journal Year: 2025, Volume and Issue: 170(6)

Published: May 9, 2025

Language: Английский

Citations

0
Detection of viruses in special stands of common ash reveals insights into the virome of Fraxinus excelsior DOI Creative Commons
Marius Rehanek,

Rim Al Kubrusli,

Kira Köpke

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 17, 2024

Abstract We report the results of a virus screening conducted on selected special stands common ash ( Fraxinus excelsior ) within FraxVir project. This project among others deals with recording and assessment diversity in impact virome dieback diseased trees. Ash dieback, fungal disease caused by invasive fungus Hymenoscyphus fraxineus is major threat to populations across Europe. Over three consecutive years, trees from different origins were tested including leaf material mother seedlings natural regeneration as well fruits gain insight into possible modes transmission. Using RT-PCR, we screened for five viruses, generalist species arabis mosaic cherry roll virus, newly discovered viruses an emaravirus, idaeovirus cytorhabdovirus group. Amplification comparison PCR amplicons comprising nucleocapsid region revealed sequence diversities that confirm two distinct cytorhadovirus present ash. Signatures novel families have been identified high-throughput sequencing. Together our provide insights distribution expand knowledge about this endangered tree species.

Language: Английский

Citations

2
First Report of Medicago Trirhavirus 1 Infecting Alfalfa in Washington State, U.S.A. DOI
Lev G. Nemchinov, Brian M. Irish, Samuel Grinstead

et al.

Plant Disease, Journal Year: 2024, Volume and Issue: 108(10), P. 3202 - 3202

Published: June 28, 2024

The first tri-segmented viruses in the family Rhabdoviridae were recently discovered by exploring publicly available plant datasets several hosts, including alfalfa (Medicago sativa L.) (Bejerman et al. 2023). They classified a novel genus "Trirhavirus" within Rhabdoviridae. trirhavirus identified was named Medicago 1 (MeTRV1). Here we report confirmation of MeTRV1 commercial fields Washington State, USA. Samples collected 2019-2021 Benton and Grant Counties, WA. leaves which virus detected displayed irregular chlorotic spotting (Fig.1). Total RNA extraction, library preparation, high throughput sequencing, bioinformatics analysis performed as described Nemchinov al (2023). Raw reads trimmed with Trimmomatic 0.39 (Bolger at 2014). SPAdes 3.15.5 (Bankevich 2012) used for assembly. four plants out 100 tested three complete segments recovered from one them. For clarity, found field samples designated MeTRV1-Wa. De novo assembly resulted contigs, which, when subjected to BLASTn analyses, aligned respective MeTRV1. contig 6,498 nucleotides (nts)-long, 99.4% identical RNA1 (BK064256.1), 5,922 mapped it (coverage 125x). MeTRV1-Wa encoded protein 2,040 amino acid (aa)-long that L (DBA36559.1, 99.8%). second 4,014 nts-long 95.2% RNA2 MetRV1 (BK064257.1) 1,751 mapping 59x). It contained open reading frames (ORFs) encoding proteins N (445 aa, 99.8%, DBA36560.1); P2 (343 99.4%, DBA36561.1); P3 (183 DBA36562.1); P4 (72 98.6%, DBA36563.1). Altogether, 4,653 third 131x) 4,889 99.1% 3 segment (BK064258.1). RNA3 proteins: P6 (274 100%, DBA36565.1); P7 (189 99.5%, DBA36566.1); P8 (514 99 %, DBA36567.1); P5 (303 99.7%, DBA36564.1). 5' trailer each had nearly 24 nts end. Genomic organization locations its ORFs are shown Fig.2. To confirm virus's presence, two sets primers designed based on predicted sequence viral segment. correct-size products amplified RT-PCR assays extracted infected (Fig.3) verified Sanger sequencing. Besides MeTRV1-Wa, sequences following known cause symptoms same library: mosaic virus, bean leafroll lucerne transient streak pea virus. Thus, observed symptomatology may not be clearly attributed due coinfecting organisms. However, possible association disease presence could suggested comparison both asymptomatic symptomatic negative Since rhabdoviruses recognized economic losses other major crops transmitted insects 2011, 2015; Jackson 2005; Man Dietzgen 2014), this experimental occurrence new U.S. is important understanding origin, distribution, pathogenic potential.

Language: Английский

Citations

1
Three-fold expansion of the genetic diversity of blunerviruses through plant (meta)transcriptome data-mining DOI

T. Sudharshan Reddy,

V. Kavi Sidharthan

Virology, Journal Year: 2024, Volume and Issue: 599, P. 110210 - 110210

Published: Aug. 29, 2024

Language: Английский

Citations

1
Two Novel Betarhabdovirins Infecting Ornamental Plants and the Peculiar Intracellular Behavior of the Cytorhabdovirus in the Liana Aristolochia gibertii DOI Creative Commons
Pedro Luis Ramos‐González, M. A. V. Alexandre,

M Potsclam-Barro

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(3), P. 322 - 322

Published: Feb. 21, 2024

Two novel members of the subfamily Betarhabdovirinae, family Rhabdoviridae, were identified in Brazil. Overall, their genomes have typical organization 3'-N-P-P3-M-G-L-5' observed mono-segmented plant-infecting rhabdoviruses. In aristolochia-associated cytorhabdovirus (AaCV), found liana aristolochia (Aristolochia gibertii Hook), an additional short orphan ORF encoding a transmembrane helix was detected between P3 and M. The AaCV genome inferred encoded proteins share highest identity values, consistently < 60%, with counterparts yerba mate chlorosis-associated virus (Cytorhabdovirus flaviyerbamate). second virus, false jalap (FaJV), herbaceous plant (Mirabilis jalapa L.) represents together tomato betanucleorhabdovirus 2, originally plants Slovenia, tentative new species genus Betanucleorhabdovirus. FaJV particles accumulate perinuclear space, electron-lucent viroplasms nuclei infected cells. Notably, distinct from rhabdoviruses, most virions to be non-enclosed within membrane-bounded cavities. Instead, they frequently seen close association surfaces mitochondria or peroxisomes. Unlike FaJV, successfully graft-transmitted healthy three Aristolochia, while mechanical seed transmission proved unsuccessful for both viruses. Data suggest that these viruses belong two Betarhabdovirinae.

Language: Английский

Citations

0
Insights into the RNA Virome of the Corn Leafhopper Dalbulus maidis, a Major Emergent Threat of Maize in Latin America DOI Creative Commons
Humberto Debat,

Esteban Simon Farrher,

Nicolás Bejerman

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(10), P. 1583 - 1583

Published: Oct. 9, 2024

The maize leafhopper (

Language: Английский

Citations

0
Identification and Characterization of a Novel Cytorhabdovirus Associated with Pear (P. communis ‘Bartlett’) Through RNA-seq DOI
Malyaj R. Prajapati, Nitika Gupta,

Pooja Thapa

et al.

Current Microbiology, Journal Year: 2024, Volume and Issue: 82(1)

Published: Dec. 16, 2024

Language: Английский

Citations

0
2.5-fold expansion of the genetic diversity of blunerviruses through plant (meta)transcriptome data-mining DOI Creative Commons

T. Sudharshan Reddy,

V. Kavi Sidharthan

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 9, 2024

Abstract Kitaviruses are plant-infecting, non-systemic disease-causing viruses with multipartite single-stranded RNA genomes. Despite their importance, knowledge on kitaviruses is limited in comparison other plant virus groups, mainly because of the lesser number identified and characterized isolates. In present study, we explored (meta)transcriptome data available public domain genome sequences fifteen putative novel blunerviruses species, including three gymnospermic monocot species. Four segments (RNAs 1–4) ten were recovered, whilst atleast two recovered for remaining viruses. Monocistronic RNA1 encodes methyltransferase (MET)-helicase (HEL) polyprotein, except Nanmu blunervirus 1 that a relatively larger MET-HEL-RNA-dependent polymerase (RdRp) polyprotein. RNAs 2 4, respectively viruses, encode HEL-RdRp polyprotein movement protein (MP). Three to four open reading frames (ORFs) determined RNA3 which conserved ORF3, always negevirus membrane (SP24) homolog. Phylogenetic analysis grouped known blunerviruses. Based organization, sequence identities encoded proteins phylogeny, regarded as new members genus Blunervirus. The study paves way initiating further studies understanding biological properties, economic importance geographical distribution

Language: Английский

Citations

0