Insights into the RNA virome of the corn leafhopperDalbulus maidis, a major emergent threat of Maize in Latin America

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 8, 2024

Abstract The maize leafhopper ( Dalbulus maidis ) is a significant threat to crops in tropical and subtropical regions, causing extensive economic losses. While its ecological interactions control strategies are well-studied, associated viral diversity remains largely unexplored. Here, we employ high-throughput sequencing data mining comprehensively characterize the D. RNA virome, revealing novel diverse viruses. We characterized six new members belonging distinct families, with evolutionary cues of beny-like viruses Benyviridae ), bunya-like Bunyaviridae iflaviruses Iflaviridae orthomyxo-like Orthomyxoviridae rhabdoviruses Rhabdoviridae ). Phylogenetic analysis places them within genus Iflavirus affinity other leafhoppers-associated iflaviruses. five-segmented highly divergent virus showed relationship insect rhabdo related rhabdo-like virus. Furthermore, belonged cluster insect-associated viruses, while bi-segmented was These results highlight existence complex virome linked paves way for future studies investigating roles, dynamics, potential biocontrol applications these on —maize pathosystem.

Language: Английский

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Insights into the RNA Virome of the Corn Leafhopper Dalbulus maidis, a Major Emergent Threat of Maize in Latin America

Viruses, Journal Year: 2024, Volume and Issue: 16(10), P. 1583 - 1583

Published: Oct. 9, 2024

The maize leafhopper (

Language: Английский

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Identification of nine putative novel members of plant-infecting alphaflexiviruses in public domain plant transcriptomes

VirusDisease, Journal Year: 2024, Volume and Issue: 35(4), P. 630 - 636

Published: Oct. 19, 2024

Language: Английский

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Translocation of the Alphanucleorhabdovirus X proteins from the cytoplasm into the nucleus through interaction with nucleocapsid protein is essential for viral pathogenesis

Phytopathology Research, Journal Year: 2024, Volume and Issue: 6(1)

Published: Nov. 19, 2024

Abstract The diverse rhabdoviruses infecting plants and animals have conserved genome organizations, the functions of viral structural proteins been extensively studied. However, increasing number found to encode various accessory proteins, whose specific roles during infection remain poorly understood. In this study, we investigated function X encoded by several members genus Alphanucleorhabdovirus . Using recently established eggplant mottled dwarf virus (EMDV) reverse genetics system, that recombinant EMDV lacking gene was able systemically infect Nicotiana benthamiana plants, albeit with reduced efficiency. deletion mutant largely restricted veinal tissues caused asymptomatic infections. protein, which localized cytoplasm when expressed alone, translocated nucleus via a interaction nucleocapsid (N) protein. Through analyzing interactions mutants phenotypes derived mutants, demonstrated carboxyl-terminal region protein (amino acids 71–83) is crucial for its N pathogenesis. Moreover, related alphanucleorhabdoviruses could fully or partially complement in infection. These findings provide new insights into plant rhabdovirus

Language: Английский

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Identification and Characterization of a Novel Cytorhabdovirus Associated with Pear (P. communis ‘Bartlett’) Through RNA-seq

Current Microbiology, Journal Year: 2024, Volume and Issue: 82(1)

Published: Dec. 16, 2024

Language: Английский

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