Molecular questioning of potential efficacy of epsilon targeted antiviral treatment option for Domestic Cat Hepadnavirus DOI Creative Commons
Bahattin Taylan Koç, Ece Adıgüzel, Tuba Çiğdem Oğuzoğlu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 30, 2024

Abstract We aimed to elucidate the molecular and secondary structure of DCH predict development antiviral drugs. performed a series polymerase chain reactions obtain complete sequences DCH. The were processed using computational tools. phylogenetic analysis showed that our belong one clade, but four are not part this monophyletic clade. A recombination detection program identified cases as potential events. cis-acting RNA region (ε) was evaluated revealed motifs similar those found in HBV. This similarity highlights for new-generation therapeutics region.

Language: Английский

Domestic cat hepadnavirus is detected infrequently in feline blood and liver samples submitted for diagnostic testing in Texas, USA DOI Creative Commons

M. Chen,

João Pedro Cavasin, Thomas Tu

et al.

Virology, Journal Year: 2025, Volume and Issue: unknown, P. 110506 - 110506

Published: March 1, 2025

Domestic cat hepadnavirus (DCH), a novel hepatitis B-like virus, has been detected in cats several regions but data are fragmented. Investigation of DCH is driven by the societal role as human companions, disease risk to sympatric endangered felids, well HBV medicine. This study investigated molecular epidemiology and sequence diversity diagnostic blood or non-paired liver samples submitted Texas. Patient age, sex, breed, neuter status, retrovirus serology results were recorded for factor analyses. Using qPCR, DNA was amplified from 3/400 (0.8 % (95 CI: 0.3-2.2 %) with virus loads 4.06 × 106, 2.33 108, 27.1 109 copies/μL blood. Feline immunodeficiency feline leukemia seroprevalence 4.3 2.5-7.1 7.5 5.0-10.8 %), respectively. A low detection rate precluded analysis. Among samples, PCR 4/303 (1.3 0.4-3.4 one which also tested positive situ hybridization. Phylogenetic analyses 3 genomes obtained this showed high homology viruses Genotype no evidence geographic clustering. study, only second USA, contributes on worldwide prevalence viremia and, context accumulating potential pathogen, supports that viremia, may vary geographically, described hepatitis-B woodchuck virus.

Language: Английский

Citations

1
Domestic cat hepadnavirus genotype B is present in Southern Brazil DOI

A. L. Tessmann,

Juliana Sumienski,

Alexandre Sita

et al.

Virus Genes, Journal Year: 2024, Volume and Issue: 61(1), P. 81 - 86

Published: Oct. 14, 2024

Language: Английский

Citations

3
Domestic Cat Hepadnavirus, a Hepatitis B-like Virus Associated with Feline Liver Disease DOI
Julia A. Beatty, Thomas Tu, John M. Cullen

et al.

Veterinary Clinics of North America Small Animal Practice, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Conserved yet Divergent Smc5/6 Complex Degradation by Mammalian Hepatitis B Virus X Proteins DOI Creative Commons
Maya Shofa,

Yoshiko Fukushima,

Akatsuki Saito

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 28, 2024

Abstract Hepatitis B virus (HBV), belonging to the genus Orthohepadnavirus , can cause chronic hepatitis and hepatocarcinoma in humans. HBV ensures optimal replication by encoding X, a multifunctional protein responsible for degrading structural maintenance of chromosome (Smc) 5/6 complex, an anti-HBV factor hepatocytes. Previous studies suggest that degradation activity Smc5/6 complex is conserved among viruses from . Recently, novel hepadnavirus cats, domestic cat (DCH) or DCH (DCHBV), has been identified be genetically close HBV. However, it remains unclear whether DCHBV X possesses similar complex–degrading activity. Here, we investigated including DCHBV, cells derived primates cats. We found degraded Smc6 several host species, degree its anti-Smc5/6 differed depending on species. Furthermore, demonstrated independently DNA-binding 1 (DDB1), which critical X–mediated degradation. Our findings highlight yet divergent machinery mammalian proteins. Importance (HBV) causes liver cancer mainly acts defense mechanism restricts viral replication. degradation, mediated across same genus. (DCHBV) exhibits capabilities. compared proteins various HBVs, different primate feline cells. The could degrade at levels. Notably, without requiring DDB1, essential These distinct strategies employed HBVs evade system, expanding our understanding adaptation persistence

Language: Английский

Citations

1
Molecular questioning of potential efficacy of epsilon targeted antiviral treatment option for Domestic Cat Hepadnavirus DOI Creative Commons
Bahattin Taylan Koç, Ece Adıgüzel, Tuba Çiğdem Oğuzoğlu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 30, 2024

Abstract We aimed to elucidate the molecular and secondary structure of DCH predict development antiviral drugs. performed a series polymerase chain reactions obtain complete sequences DCH. The were processed using computational tools. phylogenetic analysis showed that our belong one clade, but four are not part this monophyletic clade. A recombination detection program identified cases as potential events. cis-acting RNA region (ε) was evaluated revealed motifs similar those found in HBV. This similarity highlights for new-generation therapeutics region.

Language: Английский

Citations

0