Genomic Insight into the COVID-19 Severity in MAFLD Patients: A Single-Center Prospective Cohort Study DOI Open Access
Mykhailo Buchynskyi, Valentyn Oksenych, Iryna Kamyshna

et al.

Published: March 27, 2024

This study investigated the influence of single nucleotide polymorphisms (SNPs) in genes asso-ciated with interferon pathway (IFNAR2 rs2236757), antiviral response (OAS1 rs10774671, OAS3 rs10735079), and viral entry (ACE2 rs2074192) on COVID-19 severity their association non-alcoholic fatty liver disease (MAFLD). We did not observe a significant between SNPs severity. While IFNAR2 rs2236757 A allele correlated higher creatinine levels upon admission G lower band neu-trophils discharge, these findings require further investigation. The distribution OAS gene (rs10774671, rs10735079 differ MAFLD non-MAFLD patients. Our population's ACE2 rs2074192 genotypes alleles differed from European reference population. Overall, our suggest that specific may be major contributors to patient population, highlighting potential role other genetic factors environmental influences.

Language: Английский

mRNA vaccine platforms: linking infectious disease prevention and cancer immunotherapy DOI Creative Commons
Dariush Haghmorad, Majid Eslami,

Niloufar Orooji

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: March 12, 2025

The advent of mRNA vaccines, accelerated by the global response to COVID-19 pandemic, marks a transformative shift in vaccine technology. In this article, we discuss development, current applications, and prospects vaccines for both prevention treatment infectious diseases oncology. By leveraging capacity encode antigens within host cells directly, provide versatile scalable platform suitable addressing broad spectrum pathogens tumor-specific antigens. We highlight recent advancements design, innovative delivery mechanisms, ongoing clinical trials, with particular emphasis on their efficacy combating diseases, such as COVID-19, Zika, influenza, well emerging potential cancer immunotherapy. also address critical challenges, including stability, optimization immune responses, broader issue accessibility. Finally, review strategies advancing next-generation aim overcoming limitations technology enhancing preventive therapeutic approaches oncological diseases.

Language: Английский

Citations

1
Genomic insight into COVID-19 severity in MAFLD patients: a single-center prospective cohort study DOI Creative Commons
Mykhailo Buchynskyi, Valentyn Oksenych, Iryna Kamyshna

et al.

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 4, 2024

This study investigated the influence of single nucleotide polymorphisms (SNPs) in genes associated with interferon pathway (IFNAR2 rs2236757), antiviral response (OAS1 rs10774671, OAS3 rs10735079), and viral entry (ACE2 rs2074192) on COVID-19 severity their association nonalcoholic fatty liver disease (MAFLD). We did not observe a significant between SNPs severity. While IFNAR2 rs2236757 A allele was correlated higher creatinine levels upon admission G lower band neutrophils discharge, these findings require further investigation. The distribution OAS gene (rs10774671 rs10735079) differ MAFLD patients non-MAFLD patients. Our population's ACE2 rs2074192 genotypes alleles differed from that European reference population. Overall, our suggest specific may be major contributors to patient population, highlighting potential role other genetic factors environmental influences.

Language: Английский

Citations

6
Modulatory Roles of AHR, FFAR2, FXR, and TGR5 Gene Expression in Metabolic-Associated Fatty Liver Disease and COVID-19 Outcomes DOI Creative Commons
Mykhailo Buchynskyi, Valentyn Oksenych, Iryna Kamyshna

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(6), P. 985 - 985

Published: June 19, 2024

Metabolic-associated fatty liver disease (MAFLD) is a risk factor for severe COVID-19. This study explores the potential influence of gut hormone receptor and immune response gene expression on COVID-19 outcomes in MAFLD patients.

Language: Английский

Citations

5
In Silico and In Vitro Studies of the Approved Antibiotic Ceftaroline Fosamil and Its Metabolites as Inhibitors of SARS-CoV-2 Replication DOI Creative Commons
Cássia P. Delgado, Pablo A. Nogara, Milene Dias Miranda

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 491 - 491

Published: March 28, 2025

The SARS-CoV-2 proteases Mpro and PLpro are critical targets for antiviral drug development the treatment of COVID-19. 1,2,4-thiadiazole functional group is an inhibitor cysteine proteases, such as papain cathepsins. This chemical moiety also present in ceftaroline fosamil (CF), FDA-approved fifth-generation cephalosporin antibiotic. study investigates interactions between CF, its primary metabolites (M1 dephosphorylated CF M2 opened β-lactam ring) derivatives (protonated M1H M2H), open rings (open-M1H open-M2H) with evaluates CF’s effects on vitro viral replication. In silico analyses (molecular docking molecular dynamics (MD) simulations) demonstrated that potential inhibitors Mpro. Docking analysis indicated majority ligands were more stable than PLpro; however, biochemical preferred target CF. inhibited replication human Calu-3 cell model at submicromolar concentrations when added to culture medium 12 h. Our results suggest should be evaluated a repurposing agent COVID-19, considering not only but other relevant cellular pathways. Additionally, reactivity sulfur warrants further exploration protease inhibitors.

Language: Английский

Citations

0
Modulatory Roles of AHR, FFAR2, FXR, and TGR5 Gene Expression in MAFLD and COVID-19 Outcomes DOI Open Access
Mykhailo Buchynskyi, Valentyn Oksenych, Iryna Kamyshna

et al.

Published: May 13, 2024

Metabolic-associated fatty liver disease (MAFLD) is a risk factor for severe COVID-19. This study explores the potential influence of gut hormone receptor and immune response gene expression on COVID-19 outcomes in MAFLD patients. Methods: We investigated levels AHR, FFAR2, FXR, TGR5 patients with compared to controls. examined associations between clinical outcomes. Results: displayed altered AHR expression, potentially impacting recovery. Downregulated correlated increased coagulation parameters. Elevated FFAR2 linked specific cell populations hospital stay duration. Significantly lower FXR was observed both Conclusion: Our findings suggest modulatory roles MAFLD.

Language: Английский

Citations

3
Metformin in Antiviral Therapy: Evidence and Perspectives DOI Creative Commons
Iryna Halabitska, Pavlo Petakh, Oleh Lushchak

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(12), P. 1938 - 1938

Published: Dec. 18, 2024

Metformin, a widely used antidiabetic medication, has emerged as promising broad-spectrum antiviral agent due to its ability modulate cellular pathways essential for viral replication. By activating AMPK, metformin depletes energy reserves that viruses rely on, effectively limiting the replication of pathogens such influenza, HIV, SARS-CoV-2, HBV, and HCV. Its role in inhibiting mTOR pathway, crucial protein synthesis reactivation, is particularly significant managing infections caused by CMV, EBV. Furthermore, reduces oxidative stress reactive oxygen species (ROS), which are critical replicating arboviruses Zika dengue. The drug also regulates immune responses, differentiation, inflammation, disrupting life cycle HPV potentially other viruses. These diverse mechanisms suppress replication, enhance system functionality, contribute better clinical outcomes. This multifaceted approach highlights metformin’s potential an adjunctive therapy treating wide range infections.

Language: Английский

Citations

2
Genomic Insight into the COVID-19 Severity in MAFLD Patients: A Single-Center Prospective Cohort Study DOI Open Access
Mykhailo Buchynskyi, Valentyn Oksenych, Iryna Kamyshna

et al.

Published: March 27, 2024

This study investigated the influence of single nucleotide polymorphisms (SNPs) in genes asso-ciated with interferon pathway (IFNAR2 rs2236757), antiviral response (OAS1 rs10774671, OAS3 rs10735079), and viral entry (ACE2 rs2074192) on COVID-19 severity their association non-alcoholic fatty liver disease (MAFLD). We did not observe a significant between SNPs severity. While IFNAR2 rs2236757 A allele correlated higher creatinine levels upon admission G lower band neu-trophils discharge, these findings require further investigation. The distribution OAS gene (rs10774671, rs10735079 differ MAFLD non-MAFLD patients. Our population's ACE2 rs2074192 genotypes alleles differed from European reference population. Overall, our suggest that specific may be major contributors to patient population, highlighting potential role other genetic factors environmental influences.

Language: Английский

Citations

1