Characterization of Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission DOI Open Access
Luis Janssen,

Arthur Batista Silva,

Cirilo Henrique de Oliveira

et al.

Published: June 28, 2024

Studies on animal virome have mainly concentrated chordates and medically significant invertebrates, often overlooking sylvatic mosquitoes, constituting the major part of mosquito species diversity. Despite their potential role in arbovirus transmission, viromes mosquitoes remain largely unexplored. These may also harbor insect-specific viruses (ISVs), affecting arboviral transmission dynamics. The Cerrado biome, known for rapid deforestation its status as a biodiversity hotspot, offers an ideal setting investigating due to zoonotic spillover risks from land use changes. This study aimed characterize collected various locations within Minas Gerais state, Brazil. Total RNA was extracted pools Psorophora albipes, Sabethes albiprivus, Sa. chloropterus, ferox, Coquillettidia venezuelensis species, followed by high-throughput sequencing (HTS). Bioinformatic analysis included quality control, contig assembly, viral detection. Sequencing data revealed 11 near-complete genomes (new are indicated with asterisks) across seven families one unassigned genus. included: Xinmoviridae (Ferox mononega-like virus* Albipes Gordis-like virus*), Phasmaviridae (Sabethes albiprivus phasmavirus*), Lispiviridae (Pedras lispivirus isolate MG), Iflaviridae iflavivirus*), Virgaviridae (Buriti virga-like virus MG virgavirus 1*), Flaviviridae (Psorophora ferox flavivirus*), Mesoniviridae (Alphamesonivirus cavallyense genus Negevirus (Biggie negevirus*). Phylogenetic analyses evolutionary relationships varying degrees similarity sequences distinct host species. In some cases, new genera were discovered, emphasizing importance studying understand diversity evolution ecologically sensitive regions. Moreover, presence ISVs novel arboviruses underscores need ongoing surveillance control strategies mitigate risk emerging infectious diseases.

Language: Английский

Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission DOI Creative Commons
Luis Janssen,

Arthur Batista Silva,

Cirilo Henrique de Oliveira

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(8), P. 1276 - 1276

Published: Aug. 9, 2024

Studies on animal virome have mainly concentrated chordates and medically significant invertebrates, often overlooking sylvatic mosquitoes, constituting a major part of mosquito species diversity. Despite their potential role in arbovirus transmission, the viromes mosquitoes remain largely unexplored. These may also harbor insect-specific viruses (ISVs), affecting arboviral transmission dynamics. The Cerrado biome, known for rapid deforestation its status as biodiversity hotspot, offers an ideal setting investigating due to zoonotic spillover risks from land use changes. This study aimed characterize collected various locations within Minas Gerais state, Brazil. total RNA was extracted pools

Language: Английский

Citations

3
Detection and characterization of the Dezidougou virus (genus Negevirus) in mosquitoes (Ochlerotatus caspius) collected in the Republic of Sakha (Yakutia) DOI Creative Commons
Marina A. Stepanyuk, Stanislav S. Legostaev, Kristina V. Karelina

et al.

Problems of Virology, Journal Year: 2025, Volume and Issue: 70(1), P. 47 - 56

Published: March 12, 2025

Monitoring and research on arthropod-borne microorganisms is important. Recently, with the development of next-generation sequencing methods, many previously unknown viruses have been identified in insects. Isolation from mosquitoes sampled Republic Sakha (Yakutia), followed by study a new for Russia negevirus isolated species Ochlerotatus caspius, including determination its complete nucleotide sequence, phylogenetic virological characteristics. Dezidougou virus isolation was performed C6/36 (Aedes albopictus) cell culture. Electron microscopy using JEM 1400 electron microscope. Nucleotide sequence screening NGS high-throughput sequencer MiSeq, Illumina (USA). Full genome determined Sanger sequencing. Phylogenetic analysis GenBank database, Vector NTI Advance 11 MEGA programs. The replicated efficiently cells, causing their death. However, it did not replicate mammalian cultures used. cause pathologic manifestations suckling mice when infected intracerebrally. microscopic examination purified virus-containing suspension showed presence spherical viral particles diameter 45‒55 nm. results full as belonging to virus, first Côte d'Ivoire. Yakutsk 2023 strain deposited (PP975071.1). genus Negevirus characterized time Russian Federation. Further studies prevalence negeviruses, features, potential importance public health impact vector competence vectors are important promising.

Language: Английский

Citations

0
Understanding current challenges of antibiotic resistance to find new solutions for 2050: scientific, medical and political issues DOI
R Georges, Bastien Doumèche

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(7)

Published: May 29, 2025

Language: Английский

Citations

0
Characterization of Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission DOI Open Access
Luis Janssen,

Arthur Batista Silva,

Cirilo Henrique de Oliveira

et al.

Published: June 28, 2024

Studies on animal virome have mainly concentrated chordates and medically significant invertebrates, often overlooking sylvatic mosquitoes, constituting the major part of mosquito species diversity. Despite their potential role in arbovirus transmission, viromes mosquitoes remain largely unexplored. These may also harbor insect-specific viruses (ISVs), affecting arboviral transmission dynamics. The Cerrado biome, known for rapid deforestation its status as a biodiversity hotspot, offers an ideal setting investigating due to zoonotic spillover risks from land use changes. This study aimed characterize collected various locations within Minas Gerais state, Brazil. Total RNA was extracted pools Psorophora albipes, Sabethes albiprivus, Sa. chloropterus, ferox, Coquillettidia venezuelensis species, followed by high-throughput sequencing (HTS). Bioinformatic analysis included quality control, contig assembly, viral detection. Sequencing data revealed 11 near-complete genomes (new are indicated with asterisks) across seven families one unassigned genus. included: Xinmoviridae (Ferox mononega-like virus* Albipes Gordis-like virus*), Phasmaviridae (Sabethes albiprivus phasmavirus*), Lispiviridae (Pedras lispivirus isolate MG), Iflaviridae iflavivirus*), Virgaviridae (Buriti virga-like virus MG virgavirus 1*), Flaviviridae (Psorophora ferox flavivirus*), Mesoniviridae (Alphamesonivirus cavallyense genus Negevirus (Biggie negevirus*). Phylogenetic analyses evolutionary relationships varying degrees similarity sequences distinct host species. In some cases, new genera were discovered, emphasizing importance studying understand diversity evolution ecologically sensitive regions. Moreover, presence ISVs novel arboviruses underscores need ongoing surveillance control strategies mitigate risk emerging infectious diseases.

Language: Английский

Citations

1