Molecular detection and phylogenetic analysis of Mycoplasma bovis in cattle in Nineveh governorate, Iraq

˜Al-œmağallaẗ al-ʻirāqiyyaẗ li-l-ʻulūm al-bayṭariyyaẗ/Iraqi journal of veterinary sciences, Journal Year: 2024, Volume and Issue: 38(3), P. 615 - 622

Published: June 30, 2024

This study targeted to determine the prevalence of Mycoplasma bovis in cattle Nineveh Governorate, Iraq, based on three genes, including 16S rRNA, uvrC, and gapA using polymerase chain reaction (PCR) techniques, investigate phylogenetic analysis M. diagnose study. Various samples, 352 blood nasal swabs, 40 ocular 65 synovial fluid, 30 milk were randomly obtained from cattle. Based amplified rRNA gene, spp was 38.63% c-PCR technique. At same time, there no significant uvrC which 30.68% 28.69%, respectively, m-PCR No difference found between types samples for detecting bovis. The ten local sequences (5 sequence) that deposited NCBI GenBank under accession numbers OR784598.1-OR784602.1 OR792211.1-OR792215.1, with highly related 99.13-100% identity 99.81% identity, other registered different countries, Canada, Egypt, Iran, Poland, Switzerland. concludes is widespread Iraq. first highlights

Language: Английский

Prevalence of Mycoplasma bovis in Cattle in Nineveh Governorate, Iraq

Egyptian Journal of Veterinary Science, Journal Year: 2024, Volume and Issue: 56(4), P. 0 - 0

Published: May 25, 2024

The current study aimed to determine the prevalence of Mycoplasma bovis in cattle Nineveh Governorate, Iraq, using culture method, indirect enzyme-linked immunosorbent assay (i-ELISA), and conventional polymerase chain reaction (c-PCR) technique, evaluate efficiency various laboratory methods used this study. During period from March 2022 February 2023, a total 352 nasal swabs were collected randomly tested method technique (amplifying uvrC gene) for isolation molecular detection M. , respectively. Moreover, blood samples same animals antibodies against i-ELISA. results observed that was 23.57% (83 out 352) 30.68% (108 c-PCR 47.15% (166 According Kappa value (0.807), there perfect agreement between with sensitivity, specificity, accuracy 98.79%, 90.33%, 92.32% While moderate i-ELISA test based on (0.444), specificity 89.15%, 65.79%, 71.30% i-ELISA, This concluded is prevalent Governorate PCR more efficient tool than ELISA suspected samples.

Language: Английский

Analysis of Coinfection Pathogens From Foot‐and‐Mouth Disease Virus Persistently Infected Cattle Using Oxford Nanopore Sequencing

Transboundary and Emerging Diseases, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

The persistent infection caused by foot‐and‐mouth disease virus (FMDV) still lacks a reliable explanation, as its etiology and maintenance are intricate potentially involve concurrent infections with multiple pathogens. In this study, we utilized the nanopore platform for direct sequencing of clinical samples obtained from cattle persistently infected FMDV serotype O investigated distribution characteristics coinfecting pathogens in their pharyngeal region. Briefly, exploited Oxford Nanopore technology to generate high‐quality sufficient sequence data comprehensive characterization microbial genomes. Furthermore, performed comparison, alignment, phylogenetic tree construction. Our findings revealed total 23 viruses carrier bovine, FMDV, bovine orthopneumovirus, Choristoneura fumiferana granulovirus emerging top three identified analysis unexpectedly presence porcine circovirus type 2 pepper mild mottle among viral genes detected carrier. Compared noncarrier, exhibited greater diversity abundance mycoplasma types well reads counts. Therefore, propose that establishment perpetuation may be attributed simultaneous other agents mycoplasmas. These highlight significance investigating multipathogen coinfection elucidating FMD infection.

Language: Английский