A Comprehensive Review on Phage Therapy and Phage-Based Drug Development

Antibiotics, Journal Year: 2024, Volume and Issue: 13(9), P. 870 - 870

Published: Sept. 11, 2024

Phage therapy, the use of bacteriophages (phages) to treat bacterial infections, is regaining momentum as a promising weapon against rising threat multidrug-resistant (MDR) bacteria. This comprehensive review explores historical context, modern resurgence phage and phage-facilitated advancements in medical technological fields. It details mechanisms action applications phages treating MDR particularly those associated with biofilms intracellular pathogens. The further highlights innovative uses vaccine development, cancer gene delivery vectors. Despite its targeted efficient approach, therapy faces challenges related stability, immune response, regulatory approval. By examining these areas detail, this underscores immense potential remaining hurdles integrating phage-based therapies into practices.

Language: Английский

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Pulmonary Delivery of Antibiotics to the Lungs: Current State and Future Prospects

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(1), P. 111 - 111

Published: Jan. 15, 2025

This paper presents a comprehensive review of the current literature, clinical trials, and products approved for delivery antibiotics to lungs. While there are many literature reports describing potential systems, few these have translated into marketed products. Key challenges remaining high doses required and, powder formulations, ability inhaler combination deliver dose correct portion respiratory tract maximum effect. Side effects, safety concerns, disappointing trial results remain barriers regulatory approval. In this review, we describe some possible approaches address issues highlight prospects in area.

Language: Английский

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Bacteriophage therapy for multidrug-resistant infections: current technologies and therapeutic approaches

Journal of Clinical Investigation, Journal Year: 2025, Volume and Issue: 135(5)

Published: March 2, 2025

Bacteriophage (phage) therapy has emerged as a promising solution to combat the growing crisis of multidrug-resistant (MDR) infections. There are several international centers actively engaged in implementation phage therapy, and recent case series have reported encouraging success rates patients receiving personalized, compassionate for difficult-to-treat Nonetheless, substantial hurdles remain way more widespread adoption consistent success. This Review offers comprehensive overview current technologies therapeutic approaches. We first delineate common steps development, from bank establishment clinical administration, examine spectrum approaches, personalized fixed cocktails. Using framework conventional drug development pipeline, we then identify critical knowledge gaps areas such cocktail design, formulation, pharmacology, trial design. conclude that, while holds promise, structured pipeline sustained government support crucial MDR

Language: Английский

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Does Phage Therapy Need a Pan-Phage?

Pathogens, Journal Year: 2024, Volume and Issue: 13(6), P. 522 - 522

Published: June 20, 2024

The emergence of multidrug-resistant bacteria is undoubtedly one the most serious global health threats. One response to this threat that has been gaining momentum over past decade 'phage therapy'. According this, lytic bacteriophages are used for treatment bacterial infections, either alone or in combination with antimicrobial agents. However, ensure efficacy and broad applicability phage therapy, several challenges must be overcome. These encompass development methods strategies host range manipulation bypass resistance mechanisms developed by pathogenic bacteria, as case since advent antibiotics. As our knowledge understanding interactions between phages their hosts evolves, key issue define each application. In article, we discuss factors affect how determines classification into different categories action. For group, recent representative examples provided, together suggestions on groups can combat certain types infections. available methodologies expansion, through sequential adaptation a new pathogen genetic engineering techniques, also reviewed.

Language: Английский

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Metagenomics as a Transformative Tool for Antibiotic Resistance Surveillance: Highlighting the Impact of Mobile Genetic Elements with a Focus on the Complex Role of Phages

Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 296 - 296

Published: March 12, 2025

Extensive use of antibiotics in human healthcare as well agricultural and environmental settings has led to the emergence spread antibiotic-resistant bacteria, rendering many infections increasingly difficult treat. Coupled with limited development new antibiotics, rise antimicrobial resistance (AMR) caused a major health crisis worldwide, which calls for immediate action. Strengthening AMR surveillance systems is, therefore, crucial global national efforts combating this escalating threat. This review explores potential metagenomics, sequenced-based approach analyze entire microbial communities without need cultivation, transformative rapid tool improving strategies compared traditional cultivation-based methods. We emphasize importance monitoring mobile genetic elements (MGEs), such integrons, transposons, plasmids, bacteriophages (phages), relation their critical role facilitating dissemination determinants via horizontal gene transfer (HGT) across diverse environments clinical settings. In context, strengths limitations current bioinformatic tools designed detect AMR-associated MGEs metagenomic datasets, including emerging predictive machine learning models, are evaluated. Moreover, controversial phages transmission is discussed alongside phage therapy promising alternative conventional antibiotic treatment.

Language: Английский

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The regulatory framework for microbiome-based therapies: insights into European regulatory developments

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 28, 2025

The emergence of a broad spectrum microbiome-based therapies has triggered changes in European regulatory frameworks. first part the review describes these innovative therapies. second provides an overview current framework and key introduced by Regulation on substances human origin (SoHO) for development therapies, highlighting need microbiome science to unlock full potential

Language: Английский

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Integrating phage biocontrol in food production: industrial implications and regulatory overview

Deleted Journal, Journal Year: 2025, Volume and Issue: 7(4)

Published: April 7, 2025

Language: Английский

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Harnessing the Activity of Lytic Bacteriophages to Foster the Sustainable Development Goals and the “One Health” Strategy

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 549 - 549

Published: April 9, 2025

As bacteriophages (phages) are viruses that infect and destroy bacterial cells, they can be considered natural bactericides either directly or indirectly contribute to the achievement of United Nations Sustainable Development Goals (UN SDGs) on health well-being, food production security, as well environmental protection climate change mitigation, thus contributing success European “One Health” strategy combat antimicrobial resistance in humans, animals, plants, environment. The biological activity lytic operate fields microbiology biotechnology for clinical, veterinary, agricultural, industrial applications, among others, achieve proposed goals, mainly because phages help increase crop productivity by reducing diseases; constitute alternative therapies against infections caused multidrug-resistant bacteria; reduce populations pathogenic bacteria contaminate soil water, therefore ensuring healthier safer production; pollution presence agrochemicals antibiotics. Phage-based developed through research innovation have potential promote greater global security a more environmentally friendly eco-sustainable way.

Language: Английский

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Benefits and Challenges of Applying Bacteriophage Biocontrol in the Consumer Water Cycle

Microorganisms, Journal Year: 2024, Volume and Issue: 12(6), P. 1163 - 1163

Published: June 7, 2024

Bacteria (including disinfection- and antibiotic-resistant bacteria) are abundant in the consumer water cycle, where they may cause disease, lead to biofouling infrastructure damage distributions systems, subsequently resulting significant economic losses. Bacteriophages their associated enzymes then offer a biological control solution for application within sector. Lytic bacteriophages of particular interest as biocontrol agents narrow host range can be exploited targeted removal specific bacteria designated environment. also used improve processes such wastewater treatment, while bacteriophage-derived applied combat based on effectiveness against preformed biofilms. However, range, environmental stability, bacteriophage resistance biosafety risks some factors that need considered prior large-scale these bacterial viruses. Characteristics highlight potential thus outlined this review, well throughout cycle. Additionally, limitations corresponding mitigation strategies outlined, including use engineered improved ranges, stability antimicrobial re-sensitisation bacteria. Finally, public with considered, alternative applications enhance functioning quality or treatment indicators microbial source tracking markers, discussed.

Language: Английский

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Current and future directions in bacteriophage research for developing therapeutic innovations

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 17, 2024

Language: Английский

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