Comprehensive Sampling and Detection Strategies for the Field Surveillance of Tomato Brown Rugose Fruit Virus

Agronomy, Journal Year: 2025, Volume and Issue: 15(2), P. 318 - 318

Published: Jan. 27, 2025

Tomato brown rugose fruit virus (ToBRFV) poses a significant threat to tomato production. Effective and accurate detection is critical for limiting the introduction spread of ToBRFV. In this study, impact planting patterns, growth stages, cultivar variability on ToBRFV levels in tomatoes from distinct greenhouses open fields were comprehensively analyzed. The results indicated that detectable asymptomatic tissues, regardless artificial agroinoculation or natural infection. Additionally, higher viral observed newly emerging leaves fruits sepals compared old leaves. For infected with ToBRFV, level mesocarp than other interior parts, no correlation was found between color lesions. Based these results, it recommended new should be given priority testing seedlings turning stage, are suggested collected at full ripeness stage plants. This study underscores importance regular optimal sampling beyond symptom observation surveillance

Language: Английский

---

Phylodynamic of Tomato Brown Rugose Fruit Virus and Tomato Chlorosis Virus, Two Emergent Viruses in Mixed Infections in Argentina

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 533 - 533

Published: April 5, 2025

Tobamovirus fructirugosum (ToBRFV) and Crinivirus tomatichlorosis (ToCV) are emerging viral threats to tomato production worldwide, with expanding global distribution. Both viruses exhibit distinct biological characteristics transmission mechanisms that influence their spread. This study aimed reconstruct the complete genomes of ToBRFV ToCV from infected plants wastewater samples in Argentina explore evolutionary dynamics. Additionally, it compared genetic diversity plant tissue sewage samples. Using metagenomic analysis, genome sequences two isolates co-infected tomatoes, along four sewage, were obtained. The analysis showed exhibited higher environmental than Phylodynamic indicated both had a recent, single introduction but predicted different times for ancestral diversification. estimated began its diversification June 2013 Israel, rapid exponential growth until 2020, after which effective population size declined. Moreover, ToCV’s expansion was characterized by 1979 2010, Turkey identified as most probable location current data available. highlights how sequencing monitoring can enhance our understanding spread impact on agriculture.

Language: Английский

---

Tomato Brown Rugose Fruit Virus Can Be Transmissible through a Greenhouse Hydroponic System and Virus Inactivation Using a Cold Plasma Ozone Treatment

Published: March 21, 2024

Tomato brown rugose fruit virus (ToBRFV) is an emerging tobamovirus infecting tomatoes and peppers. Due to its ability trigger seed contamination, efficient mechanical transmission, break the popular resistance genes (Tm-1, Tm-2 Tm-22), has resulted in a global pandemic on greenhouse tomato production recent years despite implementation of strict hygiene disinfection measures. The objective this study was evaluate whether ToBRFV can be transmissible through recirculating hydroponic systems search for effective approach contain spread under such circumstances. We not only detected presence runoff water solutions collected from ToBRFV-infected greenhouses, but also demonstrated infectivity initiating infection plants using bioassay. then conducted treatment cold plasma-generated ozone ToBRFV-contaminated reservoir further assessed efficacy inac-tivate virus. Results showed effectiveness cold-plasma de-pendent concentrations (0.1 mg/L 1.0 mg/L), periods exposure (24 min 72 min), relative titers (1:100 1:10,000 - dilution virus-infected tissue extract), demonstrating could offer promising solution cope with potential system greenhouse.

Language: Английский

---

Tomato Brown Rugose Fruit Virus Is Transmissible through a Greenhouse Hydroponic System but May Be Inactivated by Cold Plasma Ozone Treatment

Horticulturae, Journal Year: 2024, Volume and Issue: 10(4), P. 416 - 416

Published: April 20, 2024

Tomato brown rugose fruit virus (ToBRFV) is an emerging tobamovirus infecting tomatoes and peppers, resulting in a pandemic recent years. In addition to its abilities of being seed-borne, transmitted mechanically overcoming current resistance, we speculated other factors may also contribute such catastrophic effect on tomato production hydroponic greenhouse. The objective this study was evaluate whether ToBRFV can be transmissible through recirculating systems and, more importantly, search for effective approach contain spread. We not only detected the runoff water samples collected from three greenhouses but determined virus’ infectivity bioassay. then conducted treatment using cold plasma ozone assess efficacy inactivating ToBRFV. results showed that, with high concentration (inoculum 1:100 dilution), prolonged exposure (72 min) two higher concentrations (0.6 mg/L 1.0 mg/L) achieved partial effects. With medium 1:1000 48 min sufficient completely suppress infectivity. However, low 1:10,000 inactivated even just short (24 min). Future work will need confirm against as well impact plants

Language: Английский

---

Detection of tomato brown rugose fruit virus in environmental residues: The importance of contextualizing test results

Plant Pathology, Journal Year: 2024, Volume and Issue: 73(8), P. 2071 - 2083

Published: July 14, 2024

Abstract Tomato brown rugose fruit virus (ToBRFV) is regulated as a quarantine pest in many countries worldwide. To assess whether ToBRFV present cultivations, plants or seed lots, testing required. The interpretation of test results, however, can be challenging. Reverse transcription‐quantitative (real‐time) PCR even though considered “positive”, may not always signify plant infection indicate the presence infectious virus, but could due to viral residues environment. Here, case studies from Netherlands, Belgium, and United Kingdom address questions regarding detection various settings, infectiousness ToBRFV‐positive samples. These exploratory analyses demonstrate widespread diverse samples environments. was detected inside around greenhouses with no prior history infection, on different materials surfaces, including those that were untouched by individuals, objects. This suggested dispersal through aerosols. its more often regions nearby tomato production, yet also found wider environment extending beyond infected crops. originating environmental contamination infectious, adding complexity decision‐making response positive results. Contextual information, such origin sample likelihood cultivations and/or broader environment, important for interpreting A nuanced approach crucial correctly interpret necessitating further research support risk assessment.

Language: Английский

---

Microbial Interactions for Wastewater Treatment Focusing on Microalgae-Based Systems

Published: Jan. 1, 2024

Language: Английский

---

High-Throughput Sequencing Methods for the Detection of Two Strawberry Viruses in Post-Entry Quarantine

Viruses, Journal Year: 2024, Volume and Issue: 16(10), P. 1550 - 1550

Published: Sept. 30, 2024

High-throughput sequencing (HTS) technologies may be a useful tool for testing imported plant germplasm multiple pathogens present in sample, offering strain-generic detection not offered by most PCR-based assays. Metatranscriptomics (RNAseq) and tiled amplicon PCR (TA-PCR) were tested as HTS-based techniques to detect viruses low titres. Strawberry mottle virus (SMoV), an RNA virus, strawberry vein banding (SVBV), DNA selected comparison of RNAseq TA-PCR with quantitative ribosomal RNA-depleted samples viral titre was used obtain datasets from 3 M 120 paired-end (PE) reads. demonstrated PCR-like sensitivity, able few 10 copies/µL when 60 million (M) PE reads generated. The custom primer panels designed each successfully recover the reference genomes virus. Single- multiple-target allowed around minimum continuous sequence length recovery 500 bp. limit protocols described here is comparable that This work lays groundwork increased flexibility HTS viruses.

Language: Английский

---

Genomic surveillance of Canadian airport wastewater samples allows early detection of emerging SARS-CoV-2 lineages

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 3, 2024

Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has shown wastewater (WW) surveillance to be an effective means of tracking the emergence viral lineages which arrive by many routes transmission including via transportation hubs. In Canadian province Ontario, numerous municipal treatment plants (WWTPs) participate in WW infectious disease targets such as SARS-CoV-2 qPCR and whole genome sequencing (WGS). Greater Toronto Airports Authority (GTAA), operator Pearson International Airport (Toronto Pearson), been participating since January 2022. As a major international airport Canada largest national hub, this is ideal location for globally emerging variants concern (VOCs). study, collected from Pearson’s two terminals pooled aircraft sewage was processed WGS using tiled-amplicon approach targeting virus genome. Data generated analyzed monitor trends lineage frequencies. Initial detections were compared between samples, samples surrounding regions, Ontario clinical data published Public Health Ontario. Results enabled early detection VOCs individual mutations On average, novel at preceded 1–4 weeks, up 16 weeks one case. This project illustrates efficacy transitory hubs sets example that could applied other viruses part preparedness strategy provide monitoring on mass scale.

Language: Английский

---