Discovery of novel RNA viruses through analysis of fungi-associated next-generation sequencing data DOI Creative Commons
Xiang Lü, Ziyuan Dai,

Jiaxin Xue

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: May 27, 2024

Abstract Background Like all other species, fungi are susceptible to infection by viruses. The diversity of fungal viruses has been rapidly expanding in recent years due the availability advanced sequencing technologies. However, compared virome studies, research on fungi-associated remains limited. Results In this study, we downloaded and analyzed over 200 public datasets from approximately 40 different Bioprojects explore potential fungal-associated viral dark matter. A total 12 novel sequences were identified, which RNA viruses, with lengths ranging 1,769 9,516 nucleotides. amino acid sequence identity these any known virus is below 70%. Through phylogenetic analysis, classified into orders or families, such as Mitoviridae , Benyviridae Botourmiaviridae Deltaflexiviridae Mymonaviridae Bunyavirales Partitiviridae . It possible that represent new taxa at level family, genus, species. Furthermore, a co-evolution analysis indicated evolutionary history within their groups largely driven cross-species transmission events. Conclusions These findings significant importance for understanding diversity, evolution, relationships between genome structure function further investigation needed study interactions.

Language: Английский

Ambiviricota , a novel ribovirian phylum for viruses with viroid-like properties DOI
Jens H. Kuhn, Leticia Botella, Marcos de la Peña

et al.

Journal of Virology, Journal Year: 2024, Volume and Issue: 98(7)

Published: June 10, 2024

Fungi harbor a vast diversity of mobile genetic elements (MGEs). Recently, novel fungal MGEs, tentatively referred to as 'ambiviruses,' were described. 'Ambiviruses' have single-stranded RNA genomes about 4-5 kb in length that contain at least two open reading frames (ORFs) non-overlapping ambisense orientation. Both ORFs are conserved among all currently known and one them encodes distinct viral RNA-directed polymerase (RdRP), the hallmark gene ribovirian kingdom

Language: Английский

Citations

7
Discovery of novel mycoviruses from fungi associated with mango leaf spots DOI Creative Commons
Qihua Wang, Mengyi Chen, Yanling Xie

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

Mango (Mangifera indica) is a commercially significant fruit crop cultivated globally. However, leaf spot diseases are common in mango orchards, which severely impact the yield. Mycoviruses hold promise as potential biocontrol agents. To investigate this possibility, fungi were isolated from lesions, resulting identification of six strains that contained double-stranded RNA (dsRNA). Through BLASTx analysis NCBI non-redundant database, 27 mycovirus-related contigs identified, corresponded to 10 distinct viruses grouped into 8 lineages: Alternaviridae, Chrysoviridae, Partitiviridae, Polymycoviridae, Orthototiviridae, Deltaflexiviridae, Narnaviridae, and Bunyaviricetes. Full genomic sequences these characterized confirmed be associated with their host fungi. The findings included novel mycoviruses, three previously unreported discovered new hosts, one virus strain. These results highlight diversity taxonomy mycoviruses found spots.

Language: Английский

Citations

0
Discovery of novel RNA viruses through analysis of fungi-associated next-generation sequencing data DOI Creative Commons
Xiang Lü, Ziyuan Dai,

Jiaxin Xue

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: May 27, 2024

Abstract Background Like all other species, fungi are susceptible to infection by viruses. The diversity of fungal viruses has been rapidly expanding in recent years due the availability advanced sequencing technologies. However, compared virome studies, research on fungi-associated remains limited. Results In this study, we downloaded and analyzed over 200 public datasets from approximately 40 different Bioprojects explore potential fungal-associated viral dark matter. A total 12 novel sequences were identified, which RNA viruses, with lengths ranging 1,769 9,516 nucleotides. amino acid sequence identity these any known virus is below 70%. Through phylogenetic analysis, classified into orders or families, such as Mitoviridae , Benyviridae Botourmiaviridae Deltaflexiviridae Mymonaviridae Bunyavirales Partitiviridae . It possible that represent new taxa at level family, genus, species. Furthermore, a co-evolution analysis indicated evolutionary history within their groups largely driven cross-species transmission events. Conclusions These findings significant importance for understanding diversity, evolution, relationships between genome structure function further investigation needed study interactions.

Language: Английский

Citations

3