Ambiviricota , a novel ribovirian phylum for viruses with viroid-like properties
Journal of Virology,
Journal Year:
2024,
Volume and Issue:
98(7)
Published: June 10, 2024
Fungi
harbor
a
vast
diversity
of
mobile
genetic
elements
(MGEs).
Recently,
novel
fungal
MGEs,
tentatively
referred
to
as
'ambiviruses,'
were
described.
'Ambiviruses'
have
single-stranded
RNA
genomes
about
4-5
kb
in
length
that
contain
at
least
two
open
reading
frames
(ORFs)
non-overlapping
ambisense
orientation.
Both
ORFs
are
conserved
among
all
currently
known
and
one
them
encodes
distinct
viral
RNA-directed
polymerase
(RdRP),
the
hallmark
gene
ribovirian
kingdom
Language: Английский
Discovery of novel mycoviruses from fungi associated with mango leaf spots
Frontiers in Microbiology,
Journal Year:
2025,
Volume and Issue:
16
Published: Feb. 26, 2025
Mango
(Mangifera
indica)
is
a
commercially
significant
fruit
crop
cultivated
globally.
However,
leaf
spot
diseases
are
common
in
mango
orchards,
which
severely
impact
the
yield.
Mycoviruses
hold
promise
as
potential
biocontrol
agents.
To
investigate
this
possibility,
fungi
were
isolated
from
lesions,
resulting
identification
of
six
strains
that
contained
double-stranded
RNA
(dsRNA).
Through
BLASTx
analysis
NCBI
non-redundant
database,
27
mycovirus-related
contigs
identified,
corresponded
to
10
distinct
viruses
grouped
into
8
lineages:
Alternaviridae,
Chrysoviridae,
Partitiviridae,
Polymycoviridae,
Orthototiviridae,
Deltaflexiviridae,
Narnaviridae,
and
Bunyaviricetes.
Full
genomic
sequences
these
characterized
confirmed
be
associated
with
their
host
fungi.
The
findings
included
novel
mycoviruses,
three
previously
unreported
discovered
new
hosts,
one
virus
strain.
These
results
highlight
diversity
taxonomy
mycoviruses
found
spots.
Language: Английский
Discovery of novel RNA viruses through analysis of fungi-associated next-generation sequencing data
Xiang Lü,
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Ziyuan Dai,
No information about this author
Jiaxin Xue
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et al.
BMC Genomics,
Journal Year:
2024,
Volume and Issue:
25(1)
Published: May 27, 2024
Abstract
Background
Like
all
other
species,
fungi
are
susceptible
to
infection
by
viruses.
The
diversity
of
fungal
viruses
has
been
rapidly
expanding
in
recent
years
due
the
availability
advanced
sequencing
technologies.
However,
compared
virome
studies,
research
on
fungi-associated
remains
limited.
Results
In
this
study,
we
downloaded
and
analyzed
over
200
public
datasets
from
approximately
40
different
Bioprojects
explore
potential
fungal-associated
viral
dark
matter.
A
total
12
novel
sequences
were
identified,
which
RNA
viruses,
with
lengths
ranging
1,769
9,516
nucleotides.
amino
acid
sequence
identity
these
any
known
virus
is
below
70%.
Through
phylogenetic
analysis,
classified
into
orders
or
families,
such
as
Mitoviridae
,
Benyviridae
Botourmiaviridae
Deltaflexiviridae
Mymonaviridae
Bunyavirales
Partitiviridae
.
It
possible
that
represent
new
taxa
at
level
family,
genus,
species.
Furthermore,
a
co-evolution
analysis
indicated
evolutionary
history
within
their
groups
largely
driven
cross-species
transmission
events.
Conclusions
These
findings
significant
importance
for
understanding
diversity,
evolution,
relationships
between
genome
structure
function
further
investigation
needed
study
interactions.
Language: Английский