A Label-free Optical Biosensor-Based Point-of-Care Test for the Rapid Detection of Monkeypox Virus DOI
Mete Aslan, Elif Seymour, Howard Brickner

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 5, 2024

ABSTRACT Diagnostic approaches that combine the high sensitivity and specificity of laboratory-based digital detection with ease use affordability point-of-care (POC) technologies could revolutionize disease diagnostics. This is especially true in infectious diagnostics, where rapid accurate pathogen critical to curbing spread disease. We have pioneered an innovative label-free platform utilizes Interferometric Reflectance Imaging Sensor (IRIS) technology. IRIS leverages light interference from optically transparent thin film, eliminating need for complex optical resonances enhance signal by harnessing power averaging shot-noise-limited operation achieve virtually unlimited sensitivity. In our latest work, we further improved previous ‘Single-Particle’ (SP-IRIS) technology allowing construction signature target nanoparticles (whole virus) a single image. new platform, ‘Pixel-Diversity’ (PD-IRIS), eliminated z-scan acquisition, required SP-IRIS, time-consuming expensive process, made more applicable POC settings. Using PD-IRIS, quantitatively detected Monkeypox virus (MPXV), etiological agent (Mpox) infection. MPXV was captured anti-A29 monoclonal antibody (mAb 69-126-3) on Protein G spots sensor chips were at limit-of-detection (LOD) - 200 PFU/ml (∼3.3 attomolar). PD-IRIS superior ELISA (LOD 1800 PFU/mL) used as comparator. The demonstrated using Herpes simplex virus, type 1 (HSV-1), Cowpox (CPXV). work establishes effectiveness opens possibilities its advancement clinical diagnostics Mpox POC. Moreover, modular can be adapted multiplex pathogens which high-affinity ligands are available bind their surface antigens capture them surface.

Language: Английский

A Label-free Optical Biosensor-Based Point-of-Care Test for the Rapid Detection of Monkeypox Virus DOI Creative Commons
Mete Aslan, Elif Seymour, Howard Brickner

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 269, P. 116932 - 116932

Published: Nov. 14, 2024

Diagnostic approaches that combine the high sensitivity and specificity of laboratory-based digital detection with ease use affordability point-of-care (POC) technologies could revolutionize disease diagnostics. This is especially true in infectious diagnostics, where rapid accurate pathogen critical to curbing spread disease. We have pioneered an innovative label-free platform utilizes Interferometric Reflectance Imaging Sensor (IRIS) technology. IRIS leverages light interference from optically transparent thin film, eliminating need for complex optical resonances enhance signal by harnessing power averaging shot-noise-limited operation In our latest work, we further improved previous 'Single-Particle' (SP-IRIS) technology allowing construction signature target nanoparticles (whole virus) a single image. new platform, 'Pixel-Diversity' (PD-IRIS), eliminated z-scan acquisition, required SP-IRIS, time-consuming expensive process, made more applicable POC settings. Using PD-IRIS, quantitatively detected Monkeypox virus (MPXV), etiological agent (Mpox) infection. MPXV was captured anti-A29 monoclonal antibody (mAb 69-126-3) on Protein G spots sensor chips were at limit-of-detection (LOD) - 200 PFU/mL (∼3.3 aM). PD-IRIS superior ELISA (LOD 1800 PFU/mL) used as comparator. The demonstrated using Herpes simplex virus, type 1 (HSV-1), Cowpox (CPXV). work establishes effectiveness opens possibilities its advancement clinical diagnostics Mpox POC. Moreover, modular can be adapted multiplex pathogens which high-affinity ligands are available bind their surface antigens capture them surface.

Language: Английский

Citations

6
A Label-free Optical Biosensor-Based Point-of-Care Test for the Rapid Detection of Monkeypox Virus DOI
Mete Aslan, Elif Seymour, Howard Brickner

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 5, 2024

ABSTRACT Diagnostic approaches that combine the high sensitivity and specificity of laboratory-based digital detection with ease use affordability point-of-care (POC) technologies could revolutionize disease diagnostics. This is especially true in infectious diagnostics, where rapid accurate pathogen critical to curbing spread disease. We have pioneered an innovative label-free platform utilizes Interferometric Reflectance Imaging Sensor (IRIS) technology. IRIS leverages light interference from optically transparent thin film, eliminating need for complex optical resonances enhance signal by harnessing power averaging shot-noise-limited operation achieve virtually unlimited sensitivity. In our latest work, we further improved previous ‘Single-Particle’ (SP-IRIS) technology allowing construction signature target nanoparticles (whole virus) a single image. new platform, ‘Pixel-Diversity’ (PD-IRIS), eliminated z-scan acquisition, required SP-IRIS, time-consuming expensive process, made more applicable POC settings. Using PD-IRIS, quantitatively detected Monkeypox virus (MPXV), etiological agent (Mpox) infection. MPXV was captured anti-A29 monoclonal antibody (mAb 69-126-3) on Protein G spots sensor chips were at limit-of-detection (LOD) - 200 PFU/ml (∼3.3 attomolar). PD-IRIS superior ELISA (LOD 1800 PFU/mL) used as comparator. The demonstrated using Herpes simplex virus, type 1 (HSV-1), Cowpox (CPXV). work establishes effectiveness opens possibilities its advancement clinical diagnostics Mpox POC. Moreover, modular can be adapted multiplex pathogens which high-affinity ligands are available bind their surface antigens capture them surface.

Language: Английский

Citations

3