The producer cell type of HSV-1 alters the proteomic contents and infectious capacity of virions DOI Creative Commons
Gary P. Dunn, Luke F. Domanico, Matthew P. Taylor

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

The cell that a virus replicates in i.e., the producer cell, can alter macromolecular composition and infectious capacity of virions are produced. Herpes Simplex type 1 (HSV-1) primarily infects keratinocytes epidermis or oral mucosa prior to establishing latency neurons peripheral nervous system, where persist for lifetime host. Many lines used amplify HSV-1 derived from species tissue types less physiologically relevant disease. To understand if influences infection, we tested infectivity immortalized African green monkey kidney cells (vero), human (HaCaT), primary foreskin fibroblasts (HFF-1). We observed alters produce viral proteins infected susceptibility inhibition replication by interferon treatment. HaCaT-derived consistently exhibited enhanced over HFF-1 vero-derived virus. determine changes protein virions, performed an untargeted LC/MS-MS analysis purified each line. Comparison virion associated revealed quantitative differences both cellular including ICP0, pUL24 pUL42. These results highlight influence cell-type has on infection outcomes suggest specific factors impact replication. Approximately 67% population harbors infection. study laboratories utilize several different propagate downstream experiments. virus, i.e. type, composition, immunogenicity, produced across families. found composition. Therefore, may have implications spread subsequent disease humans. Our also raise concerns about how use ceil outcome, interpretation, reproducibility experimental results.

Language: Английский

The producer cell type of HSV-1 alters the proteomic contents and infectious capacity of virions DOI Creative Commons
Gary P. Dunn, Luke F. Domanico, Matthew P. Taylor

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

The cell that a virus replicates in i.e., the producer cell, can alter macromolecular composition and infectious capacity of virions are produced. Herpes Simplex type 1 (HSV-1) primarily infects keratinocytes epidermis or oral mucosa prior to establishing latency neurons peripheral nervous system, where persist for lifetime host. Many lines used amplify HSV-1 derived from species tissue types less physiologically relevant disease. To understand if influences infection, we tested infectivity immortalized African green monkey kidney cells (vero), human (HaCaT), primary foreskin fibroblasts (HFF-1). We observed alters produce viral proteins infected susceptibility inhibition replication by interferon treatment. HaCaT-derived consistently exhibited enhanced over HFF-1 vero-derived virus. determine changes protein virions, performed an untargeted LC/MS-MS analysis purified each line. Comparison virion associated revealed quantitative differences both cellular including ICP0, pUL24 pUL42. These results highlight influence cell-type has on infection outcomes suggest specific factors impact replication. Approximately 67% population harbors infection. study laboratories utilize several different propagate downstream experiments. virus, i.e. type, composition, immunogenicity, produced across families. found composition. Therefore, may have implications spread subsequent disease humans. Our also raise concerns about how use ceil outcome, interpretation, reproducibility experimental results.

Language: Английский

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