Molecular Analysis of Human Respiratory Syncytial Virus Group B Strains Isolated in Kenya Before and During the Emergence of Pandemic Influenza A/H1N1

Influenza and Other Respiratory Viruses, Journal Year: 2025, Volume and Issue: 19(2)

Published: Feb. 1, 2025

We conducted a retrospective study to explore molecular insights into human respiratory syncytial virus (HRSV) group B strains among patients attending outpatient clinics at government medical facilities both prior and during the onset of Influenza A/H1N1/2009 pandemic outbreak. screened 2300 nasopharyngeal swabs using multiplex real time reverse transcriptase polymerase chain reaction. amplified segment first second hypervariable regions, as well conserved portion third domain G-gene HRSV-B specific primers, sequenced by Sanger di-deoxy termination method thereafter analyzed sequences. characterized circulating three known genotypes: SAB4 (1.4%), BA7 multiple variants BA9 (97.2%). The majority viruses were uniquely Kenyan with only 4% aligning lineages found elsewhere. mean evolutionary rate was estimated be 3.08 × 10-3 substitutions per site year. Our findings indicate that in Kenya underwent slower evolution period 2007-2010. Additionally, our reveal existence unique lineage new have not been reported elsewhere date.

Language: Английский

The changing landscape of acute respiratory infections in the post-pandemic era

Infectio, Journal Year: 2025, Volume and Issue: unknown, P. 59 - 60

Published: April 4, 2025

Language: Английский