Cytokine Profile Analysis During Sialodacryoadenitis Virus and Mouse Hepatitis Virus JHM Strain Infection in Primary Mixed Microglia and Astrocyte Culture—Preliminary Research DOI Creative Commons
Michalina Bartak, Weronika D. Krahel, Karolina P. Gregorczyk-Zboroch

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(9), P. 637 - 637

Published: April 25, 2025

The Coronaviridae family has again demonstrated the potential for significant neurological complications in humans during recent pandemic. In patients, these symptoms persist throughout infection, often lasting months. consequences of most post-infection might be linked with abnormal cytokine production and reactive oxygen species (ROS) expression, resulting neuron damage. We investigated effect infection Mouse Hepatitis Virus (MHV) JHM strain Sialodacryoadenitis (SDAV) on a primary microglia astrocyte culture by analysing ROS production, chemokine cell death one month post infection. For this purpose, confocal microscopy, flow cytometry, high-throughput Luminex ProcartaPlex immunopanel 48 cytokines chemokines were utilised. replication MHV-JHM SDAV astrocytes increased pro-inflammatory inhibited anti-inflammatory cytokines. expression induced two viruses differed, as did their detection after resulted much broader response compared to that MHV-JHM. Both significantly levels apoptosis small percentage cells, but without necrosis.

Language: Английский

Cytokine Profile Analysis During Sialodacryoadenitis Virus and Mouse Hepatitis Virus JHM Strain Infection in Primary Mixed Microglia and Astrocyte Culture—Preliminary Research DOI Creative Commons
Michalina Bartak, Weronika D. Krahel, Karolina P. Gregorczyk-Zboroch

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(9), P. 637 - 637

Published: April 25, 2025

The Coronaviridae family has again demonstrated the potential for significant neurological complications in humans during recent pandemic. In patients, these symptoms persist throughout infection, often lasting months. consequences of most post-infection might be linked with abnormal cytokine production and reactive oxygen species (ROS) expression, resulting neuron damage. We investigated effect infection Mouse Hepatitis Virus (MHV) JHM strain Sialodacryoadenitis (SDAV) on a primary microglia astrocyte culture by analysing ROS production, chemokine cell death one month post infection. For this purpose, confocal microscopy, flow cytometry, high-throughput Luminex ProcartaPlex immunopanel 48 cytokines chemokines were utilised. replication MHV-JHM SDAV astrocytes increased pro-inflammatory inhibited anti-inflammatory cytokines. expression induced two viruses differed, as did their detection after resulted much broader response compared to that MHV-JHM. Both significantly levels apoptosis small percentage cells, but without necrosis.

Language: Английский

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