Progress in CRISPR Technology for Antiviral Treatments: Genome Editing as a Potential Cure for Chronic Viral Infections DOI Creative Commons
Fatemeh Nouri,

Farnaz Alibabaei,

Behina Forouzanmehr

et al.

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(5), P. 104 - 104

Published: May 20, 2025

The CRISPR–Cas system has transformed molecular biology by providing precise tools for genome editing and pathogen detection. Originating from bacterial adaptive immunity, CRISPR technology identifies cleaves genetic material pathogens, thereby preventing infections. CRISPR–Cas9, the most widely utilized variant, creates double-stranded breaks in target DNA, enabling disruptions or edits. This approach shown significant potential antiviral therapies, addressing chronic infections, such as HIV, SARS-CoV-2, hepatitis viruses. In CRISPR–Cas9 edits essential viral genes disrupts latent reservoirs, while CCR5 gene modifications render T cells resistant to entry. Similarly, SARS-CoV-2 is targeted using CRISPR–Cas13d inhibit conserved genes, significantly reducing loads. Hepatitis B C treatments leverage technologies genomic regions, limiting replication expression. Emerging innovations, PAC-MAN influenza base-editing systems reduce off-target effects, further highlight therapeutic versatility of CRISPR. Additionally, advances Cas12a Cas13 have driven development diagnostic platforms like DETECTR SHERLOCK, which provide rapid cost-effective Innovative AIOD-CRISPR enable accessible point-of-care diagnostics early Experimental approaches, targeting HSV-1 transformative combating persistent

Language: Английский

Progress in CRISPR Technology for Antiviral Treatments: Genome Editing as a Potential Cure for Chronic Viral Infections DOI Creative Commons
Fatemeh Nouri,

Farnaz Alibabaei,

Behina Forouzanmehr

et al.

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(5), P. 104 - 104

Published: May 20, 2025

The CRISPR–Cas system has transformed molecular biology by providing precise tools for genome editing and pathogen detection. Originating from bacterial adaptive immunity, CRISPR technology identifies cleaves genetic material pathogens, thereby preventing infections. CRISPR–Cas9, the most widely utilized variant, creates double-stranded breaks in target DNA, enabling disruptions or edits. This approach shown significant potential antiviral therapies, addressing chronic infections, such as HIV, SARS-CoV-2, hepatitis viruses. In CRISPR–Cas9 edits essential viral genes disrupts latent reservoirs, while CCR5 gene modifications render T cells resistant to entry. Similarly, SARS-CoV-2 is targeted using CRISPR–Cas13d inhibit conserved genes, significantly reducing loads. Hepatitis B C treatments leverage technologies genomic regions, limiting replication expression. Emerging innovations, PAC-MAN influenza base-editing systems reduce off-target effects, further highlight therapeutic versatility of CRISPR. Additionally, advances Cas12a Cas13 have driven development diagnostic platforms like DETECTR SHERLOCK, which provide rapid cost-effective Innovative AIOD-CRISPR enable accessible point-of-care diagnostics early Experimental approaches, targeting HSV-1 transformative combating persistent

Language: Английский

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