Process Biochemistry, Journal Year: 2023, Volume and Issue: 131, P. 13 - 18
Published: June 1, 2023
Language: Английский
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 259, P. 129284 - 129284
Published: Jan. 9, 2024
Language: Английский
Citations
6
Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(13), P. 5053 - 5061
Published: March 24, 2023
The immunoglobulin Y (IgY) derived from hyperimmune egg yolk is a promising passive immune agent to combat microbial infections in humans and livestock. Numerous studies have been performed develop specific IgY for pathogen control, but with limited success. To date, the efficacy of commercial products, which are all delivered through an oral route, has not approved or endorsed by any regulatory authorities. Several challenging issues IgY-based immunization, were fully recognized holistically discussed previous publications, impeded development effective products animals. This review summarizes major challenges this technology, including vivo stability, purification, heterologous immunogenicity, repertoire diversity IgY. tackle these challenges, potential solutions, such as encapsulation technologies stabilize IgY, discussed. Exploration technology COVID-19 pandemic also updated review.
Language: Английский
Citations
15
Antibodies, Journal Year: 2022, Volume and Issue: 11(4), P. 62 - 62
Published: Sept. 29, 2022
Since the discovery of antibodies by Emil Von Behring and Shibasaburo Kitasato during 19th century, their potential for use as biotechnological reagents has been exploited in different fields, such basic applied research, diagnosis, treatment multiple diseases. Antibodies are relatively easy to obtain from any species with an adaptive immune system, but birds animals characterized care maintenance. In addition, they produce can be purified egg yolk, allowing a system obtaining them without performing invasive practices, which favors three "rs" animal experimentation, i.e., replacing, reducing, refining. this work, we carry out brief descriptive review most outstanding characteristics so-called "IgY technology" IgY human beings animals.
Language: Английский
Citations
21
Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13
Published: May 10, 2022
Within the framework of current COVID-19 pandemic, there is a race against time to find therapies for outbreak be controlled. Since vaccines are still tedious develop and partially available low-income countries, passive immunity based on egg-yolk antibodies (IgY) presented as suitable approach preclude potential death infected patients, its high specificity/avidity/production yield, cost-effective manufacture, ease administration. In present study, IgY recombinant RBD protein SARS-CoV-2 were produced in specific-pathogen-free chickens purified from eggs using biocompatible method. vitro immunoreactivity was tested, finding recognition neutralization values. Safety also demonstrated prior efficacy evaluation, which body weight, kinematics, histopathological assessments hamsters challenged with performed, showing protective effect administering intranasally both prophylactic treatment or post-infection treatment. The results this study showed that delivered has aid prevention overcoming infection, should very useful control advance pandemic associated mortality.
Language: Английский
Citations
19
Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown
Published: March 19, 2025
Despite the overall positive outcomes in hospitalization and mortality rates from COVID-19 vaccines, infections remained prevalent around world highlighting need for alternative control strategies. Passive immunization with chicken IgY has long served as a feasible countermeasure, which gained further popularity research community during recent pandemic. Here we demonstrate first time scalability of anti-COVID-19 production effective distribution potential use large populations. Over 70,000 chickens were immunized against SARS-CoV-2 S1 antigen to produce eggs containing anti-S1 IgY. The resulting egg yolk powder was formulated into commercially acceptable tablets human consumption. QC stability testing showed that purified maintained activity over year. batch demonstrated equal immunoreactivity virus neutralization all leading strains. Our results feasibility manufacturing edible tablets, can now be employed block viral infectivity transmission major strains affordably effectively manner both developed developing countries.
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140291 - 140291
Published: Jan. 1, 2025
Language: Английский
Citations
0
Published: Jan. 1, 2025
Language: Английский
Citations
0
Veterinary Immunology and Immunopathology, Journal Year: 2025, Volume and Issue: unknown, P. 110928 - 110928
Published: April 1, 2025
Language: Английский
Citations
0
Viruses, Journal Year: 2022, Volume and Issue: 14(5), P. 1060 - 1060
Published: May 16, 2022
Currently, SARS-CoV-2 spike receptor-binding-domain (RBD)-based vaccines are considered one of the most effective weapons against COVID-19. During first step assessing vaccine immunogenicity, a mouse model is often used. In this paper, we tested use five experimental animals (mice, hamsters, rabbits, ferrets, and chickens) for RBD immunogenicity assessments. The humoral immune response was evaluated by ELISA virus-neutralization assays. data obtained show hamsters to be least suitable candidates testing and, hence, protective efficacy RBD-based vaccines.
Language: Английский
Citations
15
Journal of Virological Methods, Journal Year: 2024, Volume and Issue: 331, P. 115062 - 115062
Published: Nov. 17, 2024
Language: Английский
Citations
2