Transdermal Blood Sampling for C-Peptide Is a Minimally Invasive, Reliable Alternative to Venous Sampling in Children and Adults With Type 1 Diabetes DOI Open Access
Rachel Besser, Anna E. Long, Katharine R. Owen

et al.

Diabetes Care, Journal Year: 2023, Volume and Issue: 47(2), P. 239 - 245

Published: Dec. 13, 2023

OBJECTIVE C-peptide and islet autoantibodies are key type 1 diabetes biomarkers, typically requiring venous sampling, which limits their utility. We assessed transdermal capillary blood (TCB) collection as a practical alternative. RESEARCH DESIGN AND METHODS Ninety-one individuals (71 with diabetes, 20 control; diabetes: aged median 14.8 years [interquartile range (IQR) 9.1–17.1], duration 4.0 [1.5–7.7]; control individuals: 42.2 [38.0–52.1]) underwent contemporaneous TCB sampling for measurement of plasma C-peptide. Participants also provided serum plasma, to glutamate decarboxylase, antigen-2, zinc transporter 8. The ability detect significant endogenous insulin secretion (venous ≥200 pmol/L) was compared along agreement in levels, using Bland-Altman. Venous detection autoantibodies, established thresholds. Acceptability by age-appropriate questionnaire. RESULTS Transdermal took mean 2.35 min (SD 1.49). Median sample volume 50 µL (IQR 40–50) 3 91 (3.3%) failures, 13 88 (14.7%) <35 µL. showed good (mean ln[C-peptide] – = 0.008, 95% CI [−0.23, 0.29], 100% [36 36] sensitivity/100% [50 50] specificity pmol/L). Where multiple autoantibody positive agreed 22 32 (sensitivity 69%), comparative 35 36 (97%). preferred (type 63% vs. 7%; 30% undecided). CONCLUSIONS testing is sensitive, specific, acceptable alternative sampling; needs further assessment.

Language: Английский

IgG antibody levels against the SARS-CoV-2 spike protein in mother–child dyads after COVID-19 vaccination DOI
María José Muñoz‐Gómez, María Martin‐Vicente,

Sara Vigil‐Vázquez

et al.

Infection, Journal Year: 2023, Volume and Issue: 52(3), P. 813 - 824

Published: Oct. 28, 2023

Language: Английский

Citations

3
Neonatal SARS-CoV-2 immunoglobulin G antibodies at delivery and their impact on COVID-19 DOI
Susanne Vetter‐Laracy, Victoria Jiménez,

M. Roldán

et al.

European Journal of Clinical Microbiology & Infectious Diseases, Journal Year: 2024, Volume and Issue: 43(4), P. 693 - 702

Published: Feb. 7, 2024

Language: Английский

Citations

0
SARS-CoV-2 anti-RBD and anti-N protein responses are differentially regulated between mother-child pairs: insight from a national study cohort at the Faroe Islands DOI Creative Commons
Ida Jarlhelt, Cecilie Bo Hansen, Laura Pérez‐Alós

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 3, 2024

Background Knowledge about SARS-CoV-2 antibody dynamics in neonates and direct comparisons with maternal responses are not well established. This study aimed to characterize directly compare the infant response a national birth cohort from Faroe Islands. Methods The levels of immunoglobulins (Ig) targeting receptor binding domain (RBD) spike protein nucleocapsid (N protein) were investigated blood umbilical cord neonates. included 537 565 mothers Islands, follow-up samples collected 12 months after birth. Multiple linear regression models used assess associations parameters neonatal Ig pregnancy outcomes. Results finding showed that acquired varying antibodies through transplacental transfer, significantly influenced by mother’s vaccination infection status. also found presence RBD associated gestational age APGAR scores. Furthermore, anti-RBD -N protein-specific during exhibited differences between children. N maintained at cohort, while only was children’s cohort. Conclusion In conclusion, SARS-CoV-2-specific immune newborns rely on immunity, persistence Igs appears be differently regulated provides new insights into underscores nuanced relationship factors humoral responses.

Language: Английский

Citations

0
Whole blood cytokine release assays reveal disparity between capillary blood sampling methods DOI Creative Commons
Rhys T Meredith,

Ross A.R. Yarham,

Hayley Mills

et al.

Clinical Biochemistry, Journal Year: 2023, Volume and Issue: 120, P. 110648 - 110648

Published: Sept. 23, 2023

The use of whole blood in rapid cytokine release assays (CRAs) is becoming an established technique for screening immune responses following natural infection or vaccination, especially the context SARS-CoV-2 pandemic. Establishing accurate capillary sampling method to replace need venipuncture could make CRAs more accessible. In this study, was collected via two different methods alongside traditional investigate whether draw affects quantification when performing CRAs.Adults previously vaccinated with vaccines donated three samples: one by venipuncture, finger prick, and a microneedle device. Whole aliquoted incubated overnight peptides left unstimulated. Cytokine plasma measured multiplex array.In unstimulated samples, little no cytokines were detected devices. Conversely, prick showed detectable levels all analysed, significantly inflated TNFα, IL-10 (p < 0.0001), IL-2, GM-CSF, IL-13 0.01), 53% these samples also positive IFN-γ. Following peptide stimulation, 25% dysregulated production IFN-γ, IL-10, lower than controls. Contrastingly, seen just 4% venous none samples.Capillary device results highly comparable those at baseline suggesting viable CRAs. differential observed prick.

Language: Английский

Citations

1
The importance of COVID-19 vaccination during lactation DOI Open Access
Maria Isabel Valverde‐Merino, Manuel Gómez‐Guzmán, Celia Piquer‐Martinez

et al.

Infectious Diseases Now, Journal Year: 2023, Volume and Issue: 54(1), P. 104831 - 104831

Published: Nov. 10, 2023

Language: Английский

Citations

0
Transdermal Blood Sampling for C-Peptide Is a Minimally Invasive, Reliable Alternative to Venous Sampling in Children and Adults With Type 1 Diabetes DOI Open Access
Rachel Besser, Anna E. Long, Katharine R. Owen

et al.

Diabetes Care, Journal Year: 2023, Volume and Issue: 47(2), P. 239 - 245

Published: Dec. 13, 2023

OBJECTIVE C-peptide and islet autoantibodies are key type 1 diabetes biomarkers, typically requiring venous sampling, which limits their utility. We assessed transdermal capillary blood (TCB) collection as a practical alternative. RESEARCH DESIGN AND METHODS Ninety-one individuals (71 with diabetes, 20 control; diabetes: aged median 14.8 years [interquartile range (IQR) 9.1–17.1], duration 4.0 [1.5–7.7]; control individuals: 42.2 [38.0–52.1]) underwent contemporaneous TCB sampling for measurement of plasma C-peptide. Participants also provided serum plasma, to glutamate decarboxylase, antigen-2, zinc transporter 8. The ability detect significant endogenous insulin secretion (venous ≥200 pmol/L) was compared along agreement in levels, using Bland-Altman. Venous detection autoantibodies, established thresholds. Acceptability by age-appropriate questionnaire. RESULTS Transdermal took mean 2.35 min (SD 1.49). Median sample volume 50 µL (IQR 40–50) 3 91 (3.3%) failures, 13 88 (14.7%) &lt;35 µL. showed good (mean ln[C-peptide] – = 0.008, 95% CI [−0.23, 0.29], 100% [36 36] sensitivity/100% [50 50] specificity pmol/L). Where multiple autoantibody positive agreed 22 32 (sensitivity 69%), comparative 35 36 (97%). preferred (type 63% vs. 7%; 30% undecided). CONCLUSIONS testing is sensitive, specific, acceptable alternative sampling; needs further assessment.

Language: Английский

Citations

0