Fc-dependent functional activity of ChAdOx1-S and CoronaVac vaccine-induced antibodies to the SARS-CoV-2 spike protein DOI Creative Commons

Alexander W. Harris,

Liriye Kurtovic,

Jeane Nogueira

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 25, 2023

Abstract Ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission and COVID-19 disease severity is influenced by immunity acquired natural exposure and/or vaccination, whereby most vaccines are formulated on the Ancestral strain. However, population-level complicated emergence of variants concern (VOCs), such as Omicron that dominant variant currently in circulation. Antibody Fc-dependent effector functions being increasingly recognised important mediators immunity, especially against VOCs. induction these populations with diverse infection vaccination histories, remains poorly defined. Here, we evaluated functional antibodies following two widely used vaccines: AstraZeneca (AZ; ChAdOx1-S) Sinovac (SV). We quantified FcγR-binding C1q-fixing spike (S) proteins Brazilian adults vaccinated AZ or SV (n=222), some which were previously exposed to SARS-CoV-2. induced greater responses S than vaccine. Previously individuals had significantly vaccine-induced compared their naïve counterparts, notably high C1q-fixation levels, irrespective vaccine type. was highest among a prior exposure, well retained protein. Overall, findings contribute our understanding its effectiveness evolving variants.

Language: Английский

Kinetics of specific anti-SARS-CoV-2 IgM, IgA, and IgG responses during the first 12 months after SARS-CoV-2 infection: A prospective longitudinal study DOI Creative Commons
Houda Amellal, Najlaa Assaid, Hicham Charoute

et al.

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(7), P. e0288557 - e0288557

Published: July 12, 2023

Coronavirus 2019 (COVID-19) is a global health threat. The kinetics of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) need to be assessed, as the long-term duration these immunoglobulins remains largely controversial. aim this study was assess longitudinal dynamics anti-SARS-CoV-2 nucleocapsid (N) protein and receptor-binding domain (RBD) spike up one year in cohort 190 COVID-19 patients. Between March September 2021, we enrolled patients from two regional hospitals Casablanca, Morocco. Blood samples were collected analyzed for antibody levels. We used commercial Euroimmun ELISA determination anti-N IgM, Abbott Architect ™ SARS-CoV-2 IgG test detection anti-RBD IgG, an in-house kit assay IgA. IgM IgA assessed 2–5, 9–12, 17–20 32–37 days after symptom onset. also 60, 90, 120 360 One-third developed (32%), while two-thirds (61%). One month onset, most with 97% 93% positivity respectively. rate remained high follow-up. However, decreased over time, only 41% testing positive year’s levels significantly higher older people (over 50 years) than other participants. found that who had received doses ChAdOx1 nCoV-19 vaccine prior infection lower response unvaccinated This difference statistically significant weeks onset symptoms. present first Africa measure (IgA, IgG) year. Most participants seropositive but showed decline titers.

Language: Английский

Citations

18
High-resolution kinetics and cellular determinants of antibody response to SARS-CoV-2 over two years after COVID-19 vaccination DOI Creative Commons
Carlota Dobaño, Rocío Rubio, Dídac Macià

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 17, 2024

Abstract Despite widespread COVID-19 vaccine coverage, breakthrough infections are increasing, mainly driven by waning immunity and the emergence of SARS-CoV-2 Omicron variants. Here, we characterized IgM, IgA IgG kinetics in 55 visits over two years post-COVID-19 vaccination, T-cell responses six months post-booster, 31 healthy adults. Antibodies to Wuhan antigens Alpha, Delta variants were quantified Luminex. SARS-CoV-2-specific measured activation-induced marker (AIM) IFN-γ/IL-2 FluoroSpot assays. Antibody trajectories varied among isotypes. decayed slowly during first subsequently slowed down. exhibited a rapid initial decay rate that decelerated stabilizing above seropositivity threshold. Contrarily, IgM rapidly dropped undetectable levels after primary vaccination. Importantly, three doses induced higher more persistent anti-spike (S) compared doses, whereas infection led superior longer-lasting anti-S or doses. subvariants had shorter persistence than ancestral virus. Finally, polyfunctional T cells correlated positively with subsequent responses. These results revealed distinct isotype non-constant highlighted benefits booster enhancing sustaining antibody

Language: Английский

Citations

2
High-resolution kinetics and cellular determinants of SARS-CoV-2 antibody response over two years after COVID-19 vaccination DOI Creative Commons
Rocío Rubio, Dídac Macià, Diana Barrios

et al.

Microbes and Infection, Journal Year: 2024, Volume and Issue: unknown, P. 105423 - 105423

Published: Sept. 1, 2024

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) studies usually rely on cross-sectional data of large cohorts but limited repeated samples, overlooking significant inter-individual antibody kinetic differences. By combining Luminex, activation-induced marker (AIM) and IFN-γ/IL-2 Fluorospot assays, we characterized the IgM, IgA, IgG kinetics using 610 samples from 31 healthy adults over two years after COVID-19 vaccination, T-cell responses six months post-booster. Antibody trajectories varied among isotypes: decayed slowly, IgA exhibited an initial sharp decline, which gradually slowed down stabilized above seropositivity threshold. Contrarily, IgM rapidly dropped to undetectable levels primary vaccination. Importantly, three vaccine doses induced higher more durable anti-spike compared doses, whereas infection led highest peak slowest decay rate Comparing with ancestral virus, recognizing Omicron subvariants had a faster decay. Finally, polyfunctional T cells were positively associated subsequent responses. These results revealed distinctive patterns by isotype highlight benefits booster in enhancing sustaining

Language: Английский

Citations

1
Comparable and sustained levels of S1-RBD-IgG and S1-RBD-IgA in BNT162b2 homologous and CoronaVac-BNT162b2 heterologous booster vaccination: A 22-month prospective study in Malaysia DOI

Anis Atifah Mohd Hisham,

Aini Syahida Mat Yassim, Rapeah Suppıan

et al.

Vaccine, Journal Year: 2024, Volume and Issue: 42(26), P. 126471 - 126471

Published: Oct. 28, 2024

Language: Английский

Citations

0
Induction of Fc-dependent functional antibodies against different variants of SARS-CoV-2 varies by vaccine type and prior infection DOI Creative Commons

Alexander W. Harris,

Liriye Kurtovic,

Jeane Nogueira

et al.

Communications Medicine, Journal Year: 2024, Volume and Issue: 4(1)

Published: Dec. 19, 2024

SARS-CoV-2 transmission and COVID-19 disease severity is influenced by immunity from natural infection and/or vaccination. Population-level complicated the emergence of viral variants. Antibody Fc-dependent effector functions are as important mediators in immunity. However, their induction populations with diverse vaccination histories against variants remains poorly defined. We evaluated functional antibodies following two widely used vaccines, AstraZeneca (AZ) Sinovac (SV), including antibody binding Fcγ-receptors complement-fixation vaccinated Brazilian adults (n = 222), some who were previously infected SARS-CoV-2, well only 200). IgG, IgM, IgA, IgG subclasses also quantified. AZ induces greater Fcγ-receptor-binding (types I, IIa, IIIa/b) than SV or infection. Previously individuals have significantly vaccine-induced responses compared to naïve counterparts. highest among a prior infection, for all receptor types, substantial complement-fixing activity seen this group. higher IgM AZ, but does not drive better activity. Some associated subject age, whereas not. Importantly, retained Omicron BA.1 S protein, being best Fcγ-receptor-1 binding, SV. Hybrid immunity, combined exposure vaccination, generates strong Fc-mediated which may contribute evolving Understanding determinants enable future vaccines efficacy different Antibodies proteins produced part immune response that identify prevent negative consequences infections. studied adults, whom had COVID-19. Differences produced, more active people There differences how effective This improved understanding could inform development improve impact Harris et al. evaluate COVID adults. Vaccine underlie observed IIIa/b), IgA production, antibodies.

Language: Английский

Citations

0
IGG and IGM response in a group of Iraqi health care worker following SARS-CoV-2 mRNA vaccine DOI Open Access

Amar Kasim Muhamad,

Russell Abo-Altemen,

Amar Muhamad

et al.

Journal of Population Therapeutics and Clinical Pharmacology, Journal Year: 2023, Volume and Issue: 30(6)

Published: Jan. 1, 2023

The global pandemic of coronavirus disease 2019 (COVID-19) is caused by infection with the SARS-CoV-2 virus.Positive detection IgM and IgG antibodies specific to has been recognized as an evidence for confirmed or vaccination.Method: One hundred healthy health care workers from both genders over eighteen years old were participated test their humeral response (IgG, IgM) after vaccinated Pfizer vaccine in vaccination center Baghdad Teaching Hospital one week second shot, matched age gender had before they get (control), groups deny recent COVID-19 infection.Result: Our finding shows that increase antibody responses following lesser extent titer than obvious our entire participant compared control.Conclusion result support successful production humoral immunity protection against infection.

Language: Английский

Citations

0
Fc-dependent functional activity of ChAdOx1-S and CoronaVac vaccine-induced antibodies to the SARS-CoV-2 spike protein DOI Creative Commons

Alexander W. Harris,

Liriye Kurtovic,

Jeane Nogueira

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 25, 2023

Abstract Ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission and COVID-19 disease severity is influenced by immunity acquired natural exposure and/or vaccination, whereby most vaccines are formulated on the Ancestral strain. However, population-level complicated emergence of variants concern (VOCs), such as Omicron that dominant variant currently in circulation. Antibody Fc-dependent effector functions being increasingly recognised important mediators immunity, especially against VOCs. induction these populations with diverse infection vaccination histories, remains poorly defined. Here, we evaluated functional antibodies following two widely used vaccines: AstraZeneca (AZ; ChAdOx1-S) Sinovac (SV). We quantified FcγR-binding C1q-fixing spike (S) proteins Brazilian adults vaccinated AZ or SV (n=222), some which were previously exposed to SARS-CoV-2. induced greater responses S than vaccine. Previously individuals had significantly vaccine-induced compared their naïve counterparts, notably high C1q-fixation levels, irrespective vaccine type. was highest among a prior exposure, well retained protein. Overall, findings contribute our understanding its effectiveness evolving variants.

Language: Английский

Citations

0