Long oligos: direct chemical synthesis of genes with up to 1,728 nucleotides

Chemical Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Low steric hindrance, low error rate: conducting synthesis on a smooth surface made direct of 1000-nucleotide oligos reality.

Language: Английский

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Intranasal hemagglutinin protein boosters induce robust mucosal immunity and cross-protection against influenza A viral challenge

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 12, 2024

Abstract Licensed parenteral influenza vaccines induce systemic antibody responses and alleviate disease severity but do not efficiently prevent viral entry transmission due to the lack of local mucosal immune responses. Here, we describe intranasal booster strategy with unadjuvanted recombinant hemagglutinin (HA) following initial mRNA-LNP vaccination, Prime HA. This regimen establishes highly protective HA-specific memory in respiratory tract. Intranasal HA boosters provided significantly reduced replication compared both young old mice. Correlation analysis revealed that slightly increased levels nasal IgA are associated a burden upper boosting an antigenically distinct H1 conferred sterilizing immunity against heterologous H1N1 virus challenge. Additionally, heterosubtypic H5 elicited cross-reactive humoral Our work illustrates potential protein as needle- adjuvant-free infection from A viruses. One Sentence Summary Adjuvant-free induces infection.

Language: Английский

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SYNTHESIS OF LONG OLIGODEOXYNUCLEOTIDES

Published: Jan. 1, 2024

Synthetic long oligodeoxynucleotides (ODNs) have found wide applications in diverse fields such as chemical biology, synthetic and genes genomes synthesis. Those leading to a significant demand for their production. However, traditional methods purifying ODNs present notable drawbacks, particularly inability purify ODNs, rendering ODN synthesis challenging. To address these issues, techniques coupled with non-chromatographic purification been developed. This technology makes the of feasible offers viable pathway producing extensive repeats or stable secondary structures, which are either unattainable exceedingly complex achieve using conventional methods. Despite advancements, use controlled pore glass (CPG) solid support poses its own limitations. In response, novel surface-functionalized materials identified mitigate challenges. Direct up 1,728-mer on surface has reported. Results indicate that surfaces eliminates steric hindrance reduces errors commonly encountered CPG. Furthermore, while catching-by-polymerization (CBP) method proven effective, affordability polymerizable tagging phosphoramidites (PTPs) remains bottleneck. overcome this hurdle, scalable four nucleoside precursors discovered, offering chromatography-free alternatives making CBP more efficient cost-effective.

Language: Английский

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Respiratorische Infektionen: Was gibt es Neues bei den Impfstoffen?

Pneumo News, Journal Year: 2024, Volume and Issue: 16(5), P. 35 - 38

Published: Oct. 29, 2024

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