The Identification of Dual T-Cell and B-Cell Epitopes Within Viral Proteins Utilizing a Comprehensive Peptide Array Approach

Vaccines, Journal Year: 2025, Volume and Issue: 13(3), P. 239 - 239

Published: Feb. 26, 2025

Background/Objectives: The development of vaccines that elicit both T-cell and B-cell responses is crucial for effective immunity against pathogens. This study introduces a novel approach to identify precise epitope peptides within viral proteins can stimulate arms the adaptive immune response, using Porcine Parvovirus (PPV) as model. Methods: Mice were infected with PPV, peptide array was utilized detect IgG signals in their sera. facilitated assessment immunogenicity PPV proteome, leading identification 14 potential candidates. These candidates then used immunize additional mice, ability induce evaluated. Results: immunization experiments identified an optimal peptide, P6, which robustly activated T cells B cells. Further analysis sub-regions this confirmed P6 most potent inducer responses. anticipated detected mice immunized highlighting efficacy our method identifying epitopes engage Conclusions: presents strategy dual by directly evaluating immunoreactivity antibodies serum. finding holds significant promise advancement epitope-based vaccines.

Language: Английский

Evaluating TGF-β1 gene expression and promoter polymorphism in cervical cancer progression

Journal of Molecular Histology, Journal Year: 2025, Volume and Issue: 56(2)

Published: April 1, 2025

Language: Английский