Postbiotic studies of mixed cultures of Schleiferilactobacillus harbinensis LH991 and Pichia kudriavzevii B-5P produced by in vitro rumen producing short-chain fatty acid DOI Creative Commons
Yetti Marlida, Joo Shun Tan, Syofyan Syofyan

et al.

Veterinary World, Journal Year: 2024, Volume and Issue: unknown, P. 2694 - 2700

Published: Nov. 1, 2024

Postbiotics are functional bioactive compounds or molecules with beneficial effects on health and activities in humans livestock, produced by probiotic bacteria yeast. Several postbiotics, including enzymes, short-chain fatty acids, amino extracellular polysaccharides, microbial cell fragments, teichoic currently being widely studied. This study aimed to explore the potential of secondary metabolites Schleiferilactobacillus harbinensis LH 991 Pichia kudriavzevii B-5P as lactic acid (LAB) yeast isolated from Budu (fermented fish) which can act postbiotics through vitro rumen fermentation. The method used a completely randomized design 5 × 4, five treatments four replications. substrate diet consisted 60% forage 40% concentrate. culture mixture was 1.3 1011 CFU/mL 50%:50% ratio S. P. B-5P. inoculum concentrations this were 0% (control), 1%, 2%, 3%, 4%. Treatments arranged based differences concentration follows: T0: control (0%); T1: 1%; T2: 2%; T3: 3%; T4: T4 group showed significant increase (p < 0.01) acids (SCFA), acetate, propionate, butyrate, valerate, isobutyrate, isovalerate compared other treatments. Meanwhile, shows that there is no > effect digestibility (in dry matter digestibility, organic crude fiber digestibility). However, highly volatile total, NH3, protein synthesis. It concluded treatment 4% (T4) containing LAB increased SCFA fermentation significantly, whereas it did not affect digestibility.

Language: Английский

A Dynamic Double‐Flow Gut‐On‐Chip Model for Predictive Absorption Studies In Vitro DOI Creative Commons
Maria Elisabetta Federica Palamà, Maurizio Aiello,

Gergő Borka

et al.

Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Abstract Human gut is crucial for digestion, drug absorption, and overall health; however traditional in vitro animal models struggle to accurately replicate its complex mechanisms. This study introduces an innovative gut‐on‐chip based on the MIVO millifluidic device, designed faithfully human intestinal environment. CaCo‐2 HT‐29 cells were co‐cultured under different ratio dynamic flow conditions, resembling bloodstream. Intestinal tissue differentiation was assessed through Trans‐epithelial electrical resistance (TEER) measurements, Zonula Occludens‐1, Alcian blue staining. After model establishment, a second applied apical side recapitulating lumen niche. The culture conditions significantly reduced cell maturation time, obtaining differentiated layer within 7‐10 days, compared 21 days of static culture. In addition, CaCo‐2:HT‐29 co‐cultures enables finely tune mucus thicknesses barrier function, essential studying specific conditions. Furthermore, introduction double apical‐basal system recapitulated permeability characteristics more closely those observed vivo. Double‐Flow Gut‐on‐Chip described successfully validated cross‐correlate function epithelial with ratios, finally providing predictive useful development disease modelling.

Language: Английский

Citations

0
Prebiotic fiber blend supports growth and development and favorable digestive health in puppies DOI Creative Commons
Allison P. McGrath, Laura A Motsinger, John Brejda

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: May 30, 2024

A healthy gastrointestinal (GI) microbiome has been shown to be essential for proper nutrient absorption and metabolism, maintenance of intestinal epithelial integrity osmolarity, gut immunomodulation, overall health. One the most effective ways promote a GI is through dietary interventions, such as addition prebiotics. Prebiotics are substrates that selectively utilized by host fermentation confer health benefit. However, research on prebiotics in companion animals limited, especially growing animals. Thus, this study was conducted assess effects novel prebiotic fiber blend key parameters related growth puppies.

Language: Английский

Citations

1
Effects of a Nutraceutical Treatment on the Intestinal Microbiota of Sled Dogs DOI Creative Commons
Benedetta Belà, Paolo Emidio Crisi, Giulia Pignataro

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(15), P. 2226 - 2226

Published: July 31, 2024

Dog sledding is the main discipline of working dogs on snow, consisting a team pulling sled under guidance owner. To carry out this sport, must have adequate nutrition and vitamin antioxidant supplementation to ensure that physical effort optimal. The present study evaluated effect sporting activity stress canine intestinal microbiota by dividing into two groups: control group did not take any nutraceutical products treated which product was administered. administered in used all cases dysbiosis it essential quickly restore balanced microbiota. results obtained show taking nutraceutical, there an increase bacteria, such as Streptococcus spp. E. coli, considered enteropathogenic detriment beneficial bacterial species Faecalibacterium spp., Turicibacter Blautia Fusobacterium Clostridium hiranonis. Instead, with displays lower amount bacteria great other for animal’s health. Microbiotal cane® can be subject intense preventing severe alterations at ecosystem levels maintaining composition possible.

Language: Английский

Citations

1
Efficient gene editing of pig embryos by combining electroporation and lipofection DOI Creative Commons
Qingyi Lin,

Nanaka Torigoe,

Bin Liu

et al.

Veterinary World, Journal Year: 2024, Volume and Issue: unknown, P. 2701 - 2707

Published: Nov. 1, 2024

Mosaicism, which is characterized by the presence of wild-type and more than one mutant allele, poses a serious problem in zygotic gene modification through clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 system. Therefore, we used pig embryos to compare editing efficiencies achieved combining electroporation lipofection using different aminopeptidase N (APN)-targeting guide RNA (gRNA) sequences. Six gRNAs (gRNA1-6) with target sequences were designed APN. Zona pellucida (ZP)-intact zygotes collected 10 h after start vitro fertilization (IVF) electroporated each gRNA their efficiency. The that lowest highest mutation rates (gRNA4 gRNA6, respectively) selected for additional assess efficiency following combined treatment. As ZP removal essential lipofection, ZP-free gRNA4 or gRNA6 IVF initiation, followed same 24 29 initiation. ZP-intact as controls. exhibited rates, respectively. gRNA4-targeted subjected initiation significantly higher total biallelic received only electroporation. Additional gRNA6-targeted had no obvious effect on rates. Electroporation low may improve embryos. However, effects vary based timing editing.

Language: Английский

Citations

0
Postbiotic studies of mixed cultures of Schleiferilactobacillus harbinensis LH991 and Pichia kudriavzevii B-5P produced by in vitro rumen producing short-chain fatty acid DOI Creative Commons
Yetti Marlida, Joo Shun Tan, Syofyan Syofyan

et al.

Veterinary World, Journal Year: 2024, Volume and Issue: unknown, P. 2694 - 2700

Published: Nov. 1, 2024

Postbiotics are functional bioactive compounds or molecules with beneficial effects on health and activities in humans livestock, produced by probiotic bacteria yeast. Several postbiotics, including enzymes, short-chain fatty acids, amino extracellular polysaccharides, microbial cell fragments, teichoic currently being widely studied. This study aimed to explore the potential of secondary metabolites Schleiferilactobacillus harbinensis LH 991 Pichia kudriavzevii B-5P as lactic acid (LAB) yeast isolated from Budu (fermented fish) which can act postbiotics through vitro rumen fermentation. The method used a completely randomized design 5 × 4, five treatments four replications. substrate diet consisted 60% forage 40% concentrate. culture mixture was 1.3 1011 CFU/mL 50%:50% ratio S. P. B-5P. inoculum concentrations this were 0% (control), 1%, 2%, 3%, 4%. Treatments arranged based differences concentration follows: T0: control (0%); T1: 1%; T2: 2%; T3: 3%; T4: T4 group showed significant increase (p < 0.01) acids (SCFA), acetate, propionate, butyrate, valerate, isobutyrate, isovalerate compared other treatments. Meanwhile, shows that there is no > effect digestibility (in dry matter digestibility, organic crude fiber digestibility). However, highly volatile total, NH3, protein synthesis. It concluded treatment 4% (T4) containing LAB increased SCFA fermentation significantly, whereas it did not affect digestibility.

Language: Английский

Citations

0