RAGE-mediated intestinal pro-inflammatory responses triggered by Giardia duodenalis

Acta Tropica, Journal Year: 2025, Volume and Issue: unknown, P. 107529 - 107529

Published: Jan. 1, 2025

Language: Английский

Giardiavirus infection alleviates growth restriction and intestinal damage caused by the intestinal parasite Giardia duodenalis

Parasites & Vectors, Journal Year: 2025, Volume and Issue: 18(1)

Published: Feb. 24, 2025

Abstract Background Giardia duodenalis is a prevalent intestinal pathogen causing giardiasis, condition characterized by diarrhea and frequently linked to malnutrition growth impairments in children. The virulence of Giardiavirus (GLV) may efficiently clear parasites from infected patients. However, we have limited understanding GLV transmission among species GLV-infected ’s impact on pathogenicity. Methods This study investigated extracellular vesicles (EVs) isolated via ultracentrifugation or exosome assay kit detect the presence EVs, results were detected using ultrastructure molecular methods, including electron microscopy, scanning quantitative polymerase chain reaction (qPCR), dot blot. Transwell migration assays confirmed spread GLV-enveloped EVs inhibitor experiments immunofluorescence. Mice gavaged with , without infection, assessed for disease progression, parameters (weight size gains), permeability, pathology. Results Parts exploit pathway reach environment, allowing these EVs. uptake GLV-containing rapid trophozoite wrapped also offers protection against external interference. Importantly, EV-coated leads divergent clinical symptoms mice, posing less risk mice reducing symptoms, such as emaciation, stunted growth, lesion damage, compared GLV-free -infected mice. Conclusions Our studies show that can species, infection alleviates lesions caused . These findings reveal could be target development novel intervention strategies Graphical abstract

Language: Английский

Role of parasite extracellular vesicles/exosomes in the interaction between hosts and virus-infected flagellate protozoa: progress and prospects

Animals and zoonoses., Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский