Bacteriocin Production by Lactiplantibacillus plantarum LD1 in Solid-State Fermentation Using Lignocellulosic Substrates

Fermentation, Journal Year: 2025, Volume and Issue: 11(4), P. 233 - 233

Published: April 21, 2025

In this study, solid-state fermentation for growth and bacteriocin production by Lactiplantibacillus plantarum LD1 was carried out using wheat bran, a lignocellulosic substrate. This is the first report showing L. in fermentation. Wheat bran supported higher of (391.69 ± 12.58 AU/mL) than other substrates. Appropriate conditions were achieved statistical designs. Significant factors identified Plackett–Burman Design their interactions studied response surface methodology. Enhanced (582.86 0.87 optimal (log10 CFU/mL 8.56 0.42) attained medium supplemented with peptone (1.13%), yeast extract glucose (1.56%), tri-ammonium citrate (0.50%). Growth non-optimized (MRS) almost similar 8.15 0.20), but level lower 0.58 AU/mL). Bacteriocin sustainable varied quantities suitability optimized bioprocess large-scale production. The cost found to be 444,583.60 AU/USD, which about 4 times more economical commercial MRS medium, 121,497.18 AU/USD). Thus, an 1.5-fold improvement as reduced approximately 25%, making economical.

Language: Английский

Effects of Adding Bacillus coagulans BCH0 to the Diet on Growth Performance, Tissue Structures, and Gut Microbiota in Broilers

Animals, Journal Year: 2025, Volume and Issue: 15(9), P. 1243 - 1243

Published: April 28, 2025

Studies demonstrated that Bacillus coagulans (B. coagulans) as a dietary additive enhanced broiler growth performance, yet its mechanisms of action modulation remained unclear. Therefore, this study investigated effects B. BCH0 (1 × 109 CFU/kg) on intestinal morphology, gut microbiota, and ileal transcriptomics in Arbor Acres broilers using completely randomized design. A total 200 one-day-old were allocated to control (Con, basal diet) experimental (BCH0, diet + 1 CFU/kg BCH0) groups (10 replicates/group, 10 birds/replicate) over 42-day trial. The results revealed significantly increased body weights (BW) at 21 42 days (p < 0.05), improved the average daily gain (ADG) during starter (1–21 days) overall phases (1–42 days), reduced ratio feed intake weight (F/G) across all 0.05). Duodenal morphology analysis indicated elevated villus height (+16.9%, p 0.01) height/crypt depth (V/C) no significant differences crypt = 0.46). In ileum, group exhibited greater 0.01), V/C 0.05) than Con group. Microbiota α-diversity or β-diversity, but phylum-level shifts involved an increase Firmicutes reduction Actinobacteriota At genus level, dominance shifted from Romboutsia (Control group) Lactobacillus (BCH0 group), accompanied by marked reductions Turicibacter, Ldatus_arthromitus, Rothia. Ileal identified 605 differentially expressed genes, with KEGG enrichment highlighting activated nutrient assimilation pathways including carbohydrate, mineral, fat, protein digestion/absorption. These findings collectively through synergistic beneficial upregulation metabolism architectural optimization, supporting role sustainable microbial for enhancing poultry productivity health.

Language: Английский

Hermetia illucens Larvae Meal Enhances Immune Response by Improving Serum Immunoglobulin, Intestinal Barrier and Gut Microbiota of Sichuan White Geese After Avian Influenza Vaccination

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(12), P. 615 - 615

Published: Dec. 2, 2024

Hermetia illucens Larvae Meal (HILM) has been observed to enhance growth performance and immune function, yet the effects mechanisms in geese remain less understood. Experiment I included 64 Sichuan White Geese investigate optimal additive amount of HILM diet, experiment II 32 access serum immunoglobulin, spleen immune-related genes, intestinal morphology gut microbiota at HILM. The results showed that addition 1% significantly increased ADG (p < 0.05), H5-R14 H7-R4 strain titer 33 d 0.01) H5-R13 0.05) 40 d, which is dose this trial. revealed IgG, IgG1, IgG2a, IgG3 complement C3 mRNA expressions IL-6 CD4 spleen. was improved, secretion SIgA expression Occludin jejunum were 0.05). Additionally, abundance Campilobacterota, Barnesiellaceae Barnesiella decreased while Lactobacillaceae This research provides new insights into use production.

Language: Английский