Editorial: Advances in veterinary tissue engineering: unlocking potential with cell-free and cell-based methods

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 26, 2025

Tissue engineering has emerged as a transformative field in veterinary medicine, offering innovative solutions for tissue repair, regeneration, and replacement (1). As traditional therapeutic approaches often fail to achieve complete repair or functional restoration, the increasingly turned interdisciplinary strategies that merge engineering, stem cell biology, biomaterial science (2,3). These innovations not only aim address limitations of conventional treatments but also emphasize sustainability, scalability, translational potential. With growing demand advanced complex injuries, degenerative diseases, congenital anomalies animals, integration cell-free cell-based methods opened new avenues research clinical application (4,5). This Research Topic serves testament dedication ingenuity researchers who are pushing boundaries this exciting field, paving way future where regeneration is reality all animals.A key theme emerging from papers published sustainable use biological resources develop cost-effective biocompatible materials applications (6). From repurposing seafood by-products scaffolds periodontal innovation while embracing principles circular economy environmental responsibility. The cells, whether derived ligament, deer antlers, human embryonic sources, further highlights potential therapies regenerative medicine (2). Additionally, investigations into biomaterial-assisted bone healing extracellular vesicle therapy aging dogs underscore impact these technologies. One promising studies written by Zivelonghi et al. explores engineering. study ingeniously repurposes sea urchin food waste create collagen-based wound dressings. By leveraging blue biotechnology, authors demonstrate feasibility extracting valuable biomaterials readily available by-products. ex vivo organ culture model, utilizing rat skin explants, effectively assessed Notably, antioxidant-enriched marine collagen dressing showed results accelerating mitigating inflammation. work marine-derived developing environmentally friendly solutions, compelling alternative dressings.Periodontal disease, including gingivitis periodontitis, prevalent oral condition caused dental plaque invasion, leading destruction tissues (7). While can reduce inflammation remove plaque, they structure. Recognizing treatments, Dai establishes protocol isolating canine ligament cells (PDLSCs) fabricates scaffold using chitosan, β-glycerol phosphate, biphasic calcium phosphate. Co-culturing PDLSCs with creates cell-scaffold demonstrated vitro biocompatibility, showing no inflammatory reactions when transplanted mandibular molar defects. PDLSC-seeded defects enhance guided significant advancement over approaches.Deer antlers exhibit unique ability, making them model studying Chen explored antler (AnSCs) medicine. remarkable capabilities have long fascinated scientists, delves mechanisms underlying phenomenon. transducing telomerase reverse transcriptase (hTERT) gene, successfully immortalized AnSCs, creating line extended proliferative capacity maintaining characteristics. small vesicles (sEVs) exhibited desirable properties, mirroring those primary AnSCs. provides resource seeking harness AnSCs their sEVs. ability generate stable scalable opens up exploring various contexts.Bone remains major focus orthopaedics, particularly managing fractures. case report Barbaro presents novel approach combination platelet-rich plasma (PRP) hydroxyapatite nanoparticles accelerate Rottweiler dog severe tibial fracture. Traditional surgical intervention failed yield satisfactory results, PRP improvements within 10 days post-treatment. Radiographic assessments increased callus formation reduced fracture gaps, highlighting therapy. insights treatment option injuries.Another important Kim evaluated cell-derived mesenchymal (ES-MSCs) (ES-MSC-EVs) improving cognitive mobility function geriatric dogs. behavioural changes Canine Cognitive Dysfunction Rating (CCDR) impairment through Liverpool Osteoarthritis Dogs (LOAD) scale before two weeks after treatment. both ES-MSC ES-MSC-EV led CCDR LOAD scores, reported adverse effects based on comprehensive blood tests. findings highlight promise strategies, vesicle-based therapies, addressing age-related neurodegenerative musculoskeletal disorders dogs.Collectively, five showcase breadth depth gene-edited allogeneic transplantation combined dogs, exemplify being diverse needs. multidisciplinary collaboration scientific discovery, we continue unlock full medicine.SummaryAdvances revolutionizing how animal regeneration. unlocking methods, clinicians challenges synergistic offers pathway achieving structural quality life advancing explore frontiers among researchers, clinicians, industry stakeholders will be essential translate advancements practical accessible patients.

Language: Английский

Advancements in Stem Cell Applications for Livestock Research: A Review

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(5), P. 397 - 397

Published: April 23, 2025

Stem cells (SCs), distinguished by their capacity for self-renewal and multipotent differentiation, represent a cornerstone of regenerative medicine. These cells, which can be categorized according to differentiation potential developmental origin, have emerged as pivotal elements in both biomedical research veterinary science. In herbivorous species, stem cell applications yielded particularly promising advances across multiple domains, including reproductive biotechnology, tissue engineering regeneration, therapeutic interventions, immunomodulation. This review synthesizes contemporary on biology five economically significant species: bovine, ovine, deer, equine, camelid. Special emphasis is placed isolation methodologies, culture optimization techniques, the molecular mechanisms governing key signaling pathways. The discussion encompasses technical impediments facing ethical framework necessary responsible scientific advancement, with particular attention animal welfare considerations development implementation cell-based technologies.

Language: Английский