Sperm Transcriptome Analysis Accurately Reveals Male Fertility Potential in Livestock

Animals, Journal Year: 2022, Volume and Issue: 12(21), P. 2955 - 2955

Published: Oct. 27, 2022

Nowadays, selection of superior male candidates in livestock as a source frozen semen based on sperm quality at the cellular level is not considered accurate enough for predicting potential fertility. Sperm transcriptome analysis approaches, such messenger RNA levels, have been shown to correlate with fertility rates. Using this technology growth has become principal method, which can be widely applied predict industry through transcriptome. It provides gene expression validate function spermatogenesis, fertilization, and embryo development, parameters This review proposes transcriptomic approach high-throughput method more accurately future.

Language: Английский

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Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility

Veterinary World, Journal Year: 2024, Volume and Issue: unknown, P. 593 - 601

Published: March 1, 2024

Background and Aim: Fertility is crucial for enhancing the efficiency of livestock production, as it directly impacts reproductive rates. A comprehensive understanding relationship between sperm quality embryo development key to optimizing outcomes improving livestock. This study analyzed developmental competence in vitro embryos recovered from Bali cattle with normal or poor motility. Materials Methods: Nine bulls fresh semen (NFS) (PFS) motility were ejaculated semen. Semen ejaculates, including volume, motility, concentration, evaluated immediately after collection measure Frozen was using computer-assisted analysis (CASA) progressive distance curve path, linear, straight line, average path velocity, curvilinear linear straightness (STR), linearity forward progression (LIN), wobble, lateral head displacement (ALH). Bull groups used determine cleavage ability fertilization cattle. Ovaries collected by slicing, only cytoplasmic oocytes compact cumulus cells this study. The matured, performed media a final concentration 1.5 × 106 spermatozoa/mL. After 48 h, cultured evaluated. Results: There significant differences values NFS PFS groups; however, there no volume concentration. difference LIN value but other CASA parameters. rate morula groups, positive correlation observed ALH. negative STR STR; variables. Conclusion: Despite variations characteristics, both demonstrated comparable embryonic competence. These findings provide important insights into fertility potential bulls, providing valuable information that can enhance selection strategies improve production. Keywords: cattle, embryos, quality.

Language: Английский

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A‐Kinase Anchor Protein 4 (proAKAP4): Protein Molecule–Based Fertility Marker of Indonesian Dairy Bull and Its Correlation With Frozen‐Thawed Sperm Quality

Veterinary Medicine International, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

The precursor protein of A-kinase anchor 4 (proAKAP4), which is abundant in the fibrous sheath sperm, plays an essential role sperm motility and has been developed into one candidate molecular-based fertility markers. This study aimed to identify proAKAP4 Indonesian dairy bulls investigate its correlation with various quality characteristics. findings are expected be applied optimize use superior bulls. Frozen semen from 20 were used, had previously classified based on their levels two groups: high (HF) low (LF). Analysis frozen-thawed quality, such as progressive total motility, kinematics, membrane integrity, mitochondrial potential, acrosome capacitation status, was carried out both groups. ProAKAP4 analyzed using Bull 4MID Kit ELISA technique. Each bull showed varying abundance (p < 0.05) another. Frozen-thawed HF higher than LF bulls, especially noncapacitated sperm. parameters capacitated acrosome-reacted opposite results, where results identification that Correlation analysis confirmed a strong association between rate. great potential marker for determining selecting

Language: Английский

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Association between sperm DNA fragmentation and fertility parameters in farm animals: a systematic review and meta-analysis

BMC Veterinary Research, Journal Year: 2025, Volume and Issue: 21(1)

Published: March 26, 2025

Accurately predicting male fertility is crucial for the animal breeding industry due to its significant economic implications. Existing literature suggests that mammalian partially dependent on sperm DNA integrity. However, routine semen analysis often fails detect damage and does not consistently correlate with field outcomes. While assessing integrity provides valuable biological insights, role in diagnosing infertility remains uncertain. This meta-analysis evaluated association between fragmentation (SDF) farm animals. Comprehensive searches were conducted using PubMed, Google Scholar, Springer Link Library, results stratified by species SDF detection methods. Across 30 studies, overall correlation coefficient (COR) was -0.46 (95% confidence interval [CI]: -0.54 -0.37; Z = -8.97; p < 0.001). A observed bulls (COR -0.47; 95% CI: -0.40; -11.13; 0.001) stallions -0.54; -0.72 -0.29; -3.83; 0.001), whereas no relationship found boars -0.19; -0.37 0.01; -1.84; 0.07). The effect of analyzed 15 demonstrating significantly higher values low-fertility animals compared high-fertility groups (SMD 0.85; 0.68 1.01; 10.07; pattern both 1.21; 0.85 1.57; 6.59; 0.64; 0.44 6.14; subgroups. These findings suggest incorporating assays into soundness evaluations could enhance accuracy selecting high-quality males artificial programs. further research adequately powered standardized methodologies, appropriate sample sizes necessary fully elucidate impact elevated

Language: Английский

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Bovine sperm HSP-70 molecules: a potential cryo-tolerance marker associated with semen quality and fertility rate

Frontiers in Veterinary Science, Journal Year: 2023, Volume and Issue: 10

Published: Aug. 9, 2023

Introduction Freezability is the ability of sperm to maintain its vitality and quality from various stress during cryopreservation process, which very important for success fertilization in AI programs. Heat shock proteins (HSPs) are unique induced response stress, including excess reactive oxygen species (ROS) oxidative damage intracellular enzymes that can harm cells. This study aimed analyze potential HSP-70 molecules bovine as a marker freezability or cryo-tolerance, well association with semen fertility rate. Methods The classification bulls based on (good freezability/GF poor freezability/PF), obtained value post-thaw viability using SYBR-14/PI-flow cytometry. Semen assessed included motility kinetics (computer-assisted analyses), plasma membrane integrity (HOS test), acrosome (FITC-PNA), mitochondrial (JC-1), DNA (Halomax kit). bull rate assessment was analyzed first service conception each derived data artificial insemination contained Indonesian-integrated National Animal Health Information System (iSIKHNAS). Gene expression levels were performed RT-qPCR method. protein abundance determined enzyme immunoassay (EIA) Results Bovine molecules, at gene level, showed higher GF ( p &lt; 0.05) than PF bulls. percentage parameter frozen–thawed significantly positively correlated 0.01) Furthermore, negatively associated low other parameters. overall closely related Conclusion We may conclude level have be developed cryo-tolerance freezability, utilized predictor

Language: Английский

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Omics strategies for unveiling male fertility-related biomarkers in livestock: A review

Gene Reports, Journal Year: 2024, Volume and Issue: 35, P. 101928 - 101928

Published: May 10, 2024

Language: Английский

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Sperm Head Morphology Alterations Associated with Chromatin Instability and Lack of Protamine Abundance in Frozen-Thawed Sperm of Indonesian Local Bulls

Animals, Journal Year: 2023, Volume and Issue: 13(15), P. 2433 - 2433

Published: July 27, 2023

This study aimed to analyze various alterations in the morphology of sperm head and its association with nucleus instability insufficient protamine. Frozen-thawed semen from twenty local Indonesian bulls was used for all stages this study. The results defect assessments are bull grouping, high (HD) low (LD). Sperm DNA damage assessed using Acridine Orange Halomax. PRM1 protein abundance carried out an enzyme immunoassay, while gene expression RT-qPCR. PRM deficiency performed CMA3. Several kinds defects HD were significantly higher (p < 0.05) than LD bulls. showed a significant difference between decreased correlated 0.01) defects, damage, abundance. lack protamine might affect nucleus's stability induce morphological head.

Language: Английский

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Identification of heat shock protein70-2 and protamine-1 mRNA, proteins, and analyses of their association with fertility using frozen-thawed sperm in Madura bulls

Animal Bioscience, Journal Year: 2023, Volume and Issue: 36(12), P. 1796 - 1805

Published: June 26, 2023

This study aims to identify heat shock protein70-2 (HSP70-2) and protamine-1 (PRM1) mRNA protein in Madura bull sperm demonstrate their relation as fertility biomarkers.The rates were grouped based on the percentage of first service conception rate (%FSCR) high (HF) (79.04%; n = 4), low (LF) (65.84%; 4). HSP70-2 PRM1 with peptidylprolyl isomerase A (PPIA) a housekeeping gene determined by quantitative real-time polymerase chain reaction, while enzyme-linked immunoassay was used measure abundance. In post-thawed semen samples, motility, viability, acrosome integrity, DNA fragmentation index analyzed. Data analysis performed measured parameters quality, relative expression, abundance PRM1, among bulls various levels (HF LF) one-way variance analysis. The Pearson correlation analyze relationship between mRNA, proteins, rate.Relative expression detected found be highly expressed HF (p<0.05) associated several quality.HSP70-2 molecules have great potential serve molecular markers for determining fertility.

Language: Английский

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Bovine nucleoprotein transitions genes and protein abundance as valuable markers of sperm motility and the correlation with fertility

Theriogenology, Journal Year: 2023, Volume and Issue: 215, P. 86 - 94

Published: Nov. 19, 2023

Language: Английский

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Sperm HSP70: may not be an age-dependent gene but is associated with field fertility in Bali bulls (Bos sondaicus)

Animal Reproduction, Journal Year: 2024, Volume and Issue: 21(2)

Published: Jan. 1, 2024

This study aimed to analyze the characteristics of HSP70 gene and protein in spermatozoa Bali bulls different age groups examine its potential as a biomarker determining bull fertility. used frozen semen produced from six divided into two based on (≤ 9 years ≥ 12 years). Parameters quality analyzed included sperm motility kinetics using computer-assisted analysis, morphological defects Diff-Quick staining, acrosome integrity FITC-PNA DNA fragmentation acridine orange staining. expression characterization was qRT-PCR, abundance enzyme immunoassays. Fertility field data were obtained by analyzing percentage conception rate for each artificial insemination service contained Indonesian-integrated system National Animal Health Information System (iSIKHNAS). The results showed significant differences (P<0.05) total progressive motility, neck midpiece, tail sperm, between bulls. no (P>0.05) groups. correlated with fertility (P<0.05). Age affected several parameters but did not affect abundance. molecule could be that determines

Language: Английский

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