Biotic Integrity Assessment of Multi-Trophic Cascade in Former Chinese Sturgeon Spawning Grounds Using Environmental DNA Metabarcoding Technology

Water, Journal Year: 2025, Volume and Issue: 17(4), P. 554 - 554

Published: Feb. 14, 2025

In comparison with traditional species identification techniques, environmental DNA (eDNA) metabarcoding technology markedly enhances the resolution and precision of classification, while simultaneously facilitating efficiency accuracy data collection. However, majority extant methods for assessing biological integrity concentrate on monitoring at a single trophic level, there is paucity systematic studies that consider multi-trophic level biomes in an integrated manner. Accordingly, aim this study was to conduct comprehensive assessment biotic communities downstream section Gezhou Dam Yangtze River mainstem, which formerly constituted Chinese sturgeon spawning grounds, utilizing eDNA technology. Furthermore, objective establish Index Biotic Integrity (IBI) system. study, we sampled 18 sampling sites three time periods, reference were selected based number factors, including distance between site outfall, extent vegetation cover, degree anthropogenic disturbance. The then compared over different periods (before, during, after season). analysis enabled construction indicators, considered fish, benthic invertebrates, zooplankton, phytoplankton, fungi, microbial (It should be noted due overlap information weak indication final indicator system will not include all taxa, but only some them). results demonstrated scores disturbance exhibited notable differences both individual whole (p < 0.05), observed tendency increased from dam. Positive linear correlation IBI concentrations sites. outcomes satisfactory indicative capacity spatial temporal consistency, providing reliable foundation ecosystem health assessment. successful application method offers novel insights tools future

Language: Английский

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Active biomonitoring of stream ecosystems: untargeted metabolomic and proteomic responses and free radical scavenging activities in mussels

Ecotoxicology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Language: Английский

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Environmental DNA metabarcoding: Current applications and future prospects for freshwater fish monitoring

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 376, P. 124531 - 124531

Published: Feb. 16, 2025

Language: Английский

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Spatial and Temporal Variations in Phytoplankton Community in Dianchi Lake Using eDNA Metabarcoding

Water, Journal Year: 2023, Volume and Issue: 16(1), P. 32 - 32

Published: Dec. 21, 2023

The growth and reproduction of phytoplankton are closely associated with the changes water environment; thus, have been taken as environmental indicator organisms provided references for environment protection. However, community characteristics Dianchi Lake (a seriously polluted lake in China) unclear under background cumulative effects historical pollutants current control measures, DNA (eDNA) metabarcoding monitoring has rarely applied research at Lake. Therefore, this study investigated temporal spatial stressors via eDNA monitoring. A total 10 phyla, 22 classes, 50 orders, 82 families, 108 genera species were detected, distinct variations (e.g., ASV number, dominant taxon, relative abundance) observed Microcystis dominated prokaryotic from dry period to wet period, but interestingly, first cyanobacteria genus was changed (dry period) Planktothrix (wet period). Cryptophyta eukaryotic eukaryotic-phytoplankton-dominant included Cryptomonas, Aulacoseira, Plagioselmis others. temporal–spatial heterogeneity relationships between factors shown Dissolved oxygen crucial stressor influencing structure during while pH one period. impacts phosphorus nitrogen also showed differences different periods. This provides an interesting perspective on diversity health assessment restoration

Language: Английский

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Content of Fatty Acids in Relation to the Metal Concentration in the Muscle of Two Freshwater Fish Species

Published: July 27, 2023

In this study, the concentrations of metals (As, Cd, Co, Cr, Cu, Hg, Ni, Pb, and Zn) were deter-mined in two freshwater fish species. The same also used to determine content fatty acids (FA), including saturated (SFA), monounsaturated (MUFA), polyunsaturated (PUFA). We investigated species-specific relationship between bioaccumulation FA. Roach white bream caught at different sites Serbian part Danube. measured metal below standard recommended guidelines EU Republic Ser-bia. Among non-essential metals, Ni had highest concentration, ranging from 0.158 0.591 µg g-1. muscle species contained high SFA, 36.31 51.79%, MUFA, 33.56 43.70%. Polyunsaturated represented with lowest percentage (5.32 - 27.18). Four nine studied Pb) one five (A, Pb other showed correlations acid content. These results help reveal potential biomarkers stress lipid composition.

Language: Английский

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Fish embryo toxicity of cyanobacteria via mitochondria-mediated oxidative damage

Hydrobiologia, Journal Year: 2024, Volume and Issue: 851(17), P. 4269 - 4283

Published: June 1, 2024

Language: Английский

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Evaluation of haematological and biochemical stress biomarkers and physiological parameters in Carassius gibelio caught in the coastal zone of the Curonian lagoon

Published: June 12, 2024

This study aimed to assess the health status of Carassius gibelio, a comparatively tolerant fish species, in largest coastal lagoon Europe – Curonian Lagoon. Haematocrit and glucose levels were evaluated as reliable biomarkers stress responses fish. Additionally, non-specific physiological biomarkers, including hepatosomatic index condition factor, utilized environmental quality Fish samples collected from two sites: southern part Klaipėda port, known Kiaulės Nugara Island, central Lagoon near Ventė Cape. Based on multi-biomarker C. differences observed between sampling sites, but not statistically significant. Consequently, gibelio cannot be recommended suitable bioindicator pollution is for biomonitoring surveys

Language: Английский

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Application of Synchronous Evaluation-Diagnosis Model with Quantitative Stressor-Response Analysis (SED-QSR) to Urban Lake Ecological Status: A Proposed Multiple-Level System

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(36), P. 16028 - 16039

Published: Aug. 29, 2024

Ecological integrity assessment and degradation diagnosis are used globally to evaluate the health of water bodies pinpoint critical stressors. However, current studies mainly focus on separate evaluation or diagnosis, leading an inadequate exploration relationship between stressors responses. Here, based multiple data sets in urban lake system, a synchronous evaluation-diagnosis model with quantitative stressor-response analysis was advanced, aiming improve accuracy diagnosis. The weights for key physicochemical were quantitatively determined sequence NDAVIadj > CODMn TP NH4+–N by combination generalized additive structural equation modeling, clarifying most significant effects aquatic vegetation fish assemblages. Then, sensitive biological metrics screened considering distinct contributions four alleviate possible deviation caused common methods. Finally, ecological evaluated summing according model-deduced instead empirical weights. Our system's results achieved over 80% when predicting anthropogenic stress status, which highlights great potential our multiple-level system ecosystem management.

Language: Английский

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Active Biomonitoring of Stream Ecosystems: Untargeted Metabolomic and Proteomic Responses and Free Radical Scavenging Activities in Mussels

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 25, 2024

Many contaminants from scattered sources constantly endanger streams that flow through heavily inhabited areas, commercial districts, and industrial hubs. The responses of transplanted mussels in active biomonitoring programs will represent the dynamic environmental stream conditions. This study evaluated untargeted metabolomic proteomic free radical scavenging activities Sinanodonta woodiana Winongo Stream at three stations (S1, S2, S3) representing different pollution levels: low (S1), moderate (S3), high (S2). investigation examined gills 2,2-diphenyl-1-picrylhydrazyl (DPPH) 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) gills, mantle, digestive glands. Metabolomic analysis revealed a clear separation between mussel after 28 days exposure, with specific metabolites responding to levels. Proteomic identified β-Actin protein all stations. sequence on unexposed has coverage 17%, increased 23% S1 Day 34% S2 S3 28. All tissues showed DPPH ABTS day 3 28, mainly S3. These findings underscore impact levels S. importance these discoveries as early indicators long-term aquatic problems. In face current challenges, this research raises concerns about health water bodies. It underscores developing robust, standardized, dependable analytical techniques for monitoring environments.

Language: Английский

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Advancements in Biomonitoring and Remediation Treatments of Pollutants in Aquatic Environments, 2nd Edition

Applied Sciences, Journal Year: 2023, Volume and Issue: 13(17), P. 9737 - 9737

Published: Aug. 28, 2023

Worldwide anthropogenic activities continuously produce and release hundreds of potentially toxic chemicals that contaminate ecosystems, leaving devastating effects on the environment living beings, humans included [...]

Language: Английский

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